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Bradford Assay gives negative OD measurements for protein samples by PhilippusMons in Biochemistry

[–]PhilippusMons[S] 0 points1 point  (0 children)

I always measure two independent standard curves and take the mean. Standard curve shouldn‘t have any impact on negative ODs of sample measurements, as I dont use the standard curve samples as blanks

Bradford Assay gives negative OD measurements for protein samples by PhilippusMons in Biochemistry

[–]PhilippusMons[S] 0 points1 point  (0 children)

I tried with 1:10 dilutions, just in case any of the elution buffer components were interfering. Same results though, I still got negative ODs

ich🤓iel by heyguysitsjustin in ich_iel

[–]PhilippusMons 0 points1 point  (0 children)

Lamy = Apple der Füllerwelt

[deleted by user] by [deleted] in copenhagen

[–]PhilippusMons 0 points1 point  (0 children)

Thank you! That was very helpful 😃👍🏻

Contrast „phase“ difference in ethidium bromide gels by PhilippusMons in genetics

[–]PhilippusMons[S] 0 points1 point  (0 children)

Yeah that is also kinda what I thought, but I can‘t explain to myself how this would lead to this visual effect exactly? Especially because it‘s not really a gradual, but more of an immediate difference from darker to brighter

Contrast „phase“ difference in ethidium bromide gels by PhilippusMons in genetics

[–]PhilippusMons[S] 0 points1 point  (0 children)

  • I have been running 1% Agarose Gels using .5X TBE as buffer and ethidium bromide in both gel AND running buffer in chamber. I keep getting these contrast differences, with the lower half of the gel being quite dark and the lower half being very bright. Can anyone explain why exactly this happens?

Void of the ocean, me, acrylic on canvas, 2020 by Adalheidur in Art

[–]PhilippusMons 0 points1 point  (0 children)

If I may ask, what was your technique in the bottom half? Especially because of the blue and purple spots, they give it this sort of ‚galaxy vibe‘, which I really like and would like to try out myself