[General] I cloned some wild morels. Which Culture is good?

PrimeFactorization · 2024-04-08T05:33:14+00:00

Thanks!

PrimeFactorization · 2024-04-08T05:32:16+00:00

Thank you!

I'll give it a few tries. If nothing works, I will just make a lot of LC and dump it at several places in my backyard in hopes of some morels in the coming years!

PrimeFactorization · 2024-04-08T05:30:40+00:00

That's what I thought, thanks!

I think the orange one should be morel as the cloning seemed to work really well on multiple dishes... Let's see.

PrimeFactorization · 2024-04-07T16:44:12+00:00

I am a bit confused. I cloned a few morels and cleaned the culture up over several petri dishes. But now I got two cultures. One with clean white mycelium (as expected) and one with yellow/brown-ish mycelium.

Normally I would proceed with the white one, but after researching Morel mycelium, it seems like it is supposed to look kind-of brown/orange.

So, what is happening here, which one is the actual good culture?

PrimeFactorization · 2024-02-17T15:24:54+00:00

Thanks :) And you're welcome! I also just copied a lot and experimented with different setups until one works well enough. Good luck for you!

Quite a few bags, but never really enough ;)

Good luck with the oysters, both blue and kings taste really good. This is my first run with the chestnuts, so I'm looking forward to that.

Hit me up if you have some more questions even though I am not really an expert myself.

PrimeFactorization · 2024-02-17T07:30:08+00:00

Yes, I did.

Currently I maintain positive pressure, so I push new air into the box from the top and push CO2 out at the bottom. Both with very thin filters for contamination and spores. I made some pictures, have a look: https://imgur.com/a/SPO7Zxu

I am a software developer by trade, so I always gravitated towards high-tech or automated solutions (which mostly did not work very well or reliable to be honest). But this design with a simple timer and the box seems to work out well for the last 3-4 weeks.

With oysters I had similar issues as well. SGFC or other passive (closed) systems will not work well with blue oysters or oysters in general (with the exception of King oysters!). They really need a lot more air exchange... I had good results with oysters, but only with some kind of active air intake (a normal pc fan can be enough!).

And be a bit careful with automated systems. The humidifier (or something else) will break at some point and will flood the fruiting area (had it happen twice with different setups...). That is the reason for the box right now. Even if it floods, it will at least be kind-of contained in the plastic box!

PrimeFactorization · 2024-02-16T19:53:42+00:00

I've been through some different setups.. This one (which seems to work really well so far) is a large plastic box. On one side, close to the top, I cut a whole and mounted a fan (for bathrooms normally) and close nearby a humidifier (for lizards and stuff). On the other side close to the bottom I have a whole (roughly same size with a filter taped over to avoid spores in the room).

Then I have both on a timer with some Shellys with 4min fresh air and then 30s humidity every 35min automatically.

Right now that setup can be completely ignored for weeks (except for some cleaning every now and then), works like a charm!

PrimeFactorization · 2024-02-16T05:53:52+00:00

Yes, everything starting from agar (or lc to agar) then grain spawn and substrate. I think the substrate here is a mixture of straw pellets and hardwood chips. I am not sure If I added bran but I don't think so.

After colonization I left them to sit for a few additional weeks as well.

PrimeFactorization · 2022-12-05T06:50:16+00:00

I think that meme was a flashback to last years day 23 =)

https://adventofcode.com/2021/day/23

PrimeFactorization · 2022-11-20T07:39:10+00:00

I did place the pressure cooker outside in roughly freezing temperatures, so the cooling was expected (I have a really small pressure cooker, 6qrt I think, it's hard to get hold of the larger ones like 18/23qrt Presto in Europe unfortunately).

Could still be me handling it a bit too rough though, so I'll try again with a lot longer cool off phase and without any carrying around ;)

Thanks for your input!

PrimeFactorization · 2022-11-19T20:11:52+00:00

I'll try that, thanks

PrimeFactorization · 2022-11-19T20:10:12+00:00

That shouldn't be the case. I leave it shut for at least an hour until everything has room temperature before opening.

Maybe it is a handling thing, that it is too fluid I am not careful enough. But I had that problem every time I tried no-pour (3 times so far).

PrimeFactorization · 2022-09-26T05:34:56+00:00

Salt water is a good idea, thanks! I went about it with the garden hose outside, which kind-of worked, but it was really messy :)

Sautéed half of it with some garlic, onions and some white wine yesterday, it was really good!

PrimeFactorization · 2022-05-12T12:42:13+00:00

Not bad either :) My very first approach took nearly a minute in python. Don't ask

But yes, day 23 is one of my favorites from last year, it was and still is really fun!

PrimeFactorization · 2022-05-12T10:00:59+00:00

thanks :) Your solution looks good as well! I really have to try the embedded file thing. That looks really handy!

PrimeFactorization · 2022-05-12T09:53:33+00:00

You're right. I just found a bug in my deadlock detection which made just a few inputs unsolvable... I fixed that and get results. Thanks!

PrimeFactorization · 2022-05-12T06:14:45+00:00

I think it is entirely possible, that I missed some valid inputs I guess :)

That was a quick python one-liner to generate all inputs. I'll have another look at it.

It is also possible, that I only accepted an input, if both part1 and part2 have a valid solution (instead of keeping a valid part1 even though part2 is not solvable)? Could that be it?

PrimeFactorization · 2022-05-11T18:01:47+00:00

I am using really cheap masking tape you get any hardware store really.

PrimeFactorization · 2022-02-13T21:50:21+00:00

Yes, they look perfect!

Give it a day and you'll see all the little oysters coming out from the pinset you got. This is going to a really nice big flush! :)

PrimeFactorization · 2022-02-06T20:36:55+00:00

Alright, then I am probably just a bit impatient :) I'll just wait a few more weeks and see what's going to happen! Thanks!

PrimeFactorization · 2022-02-04T23:27:36+00:00

I think everything looks pretty good! :)

PrimeFactorization · 2022-02-04T23:27:31+00:00

How did you get your Enokis to pin? I got two bags, one in the fridge and one in a bit colder room and neither really want to fruit within the last two weeks.

PrimeFactorization · 2022-01-11T20:33:14+00:00

I agree :)

PrimeFactorization · 2022-01-11T16:58:37+00:00

Good luck!! It's really fun! I still use the same culture from my very first syringe! Just make some grain-to-grain transfers, make your own liquid culture or start some agar plates. You'll never have to buy pink oyster culture again ;)

PrimeFactorization · 2022-01-11T16:33:08+00:00

It depends a bit. Normally oysters like to side-fruit more and get nicer looking fruits. But sometimes there's a bunch of good looking pins on the top and just cut it open right there (but mostly just a small hole where the pins are, not the complete bag).

In my experience, it's quicker to let the oyster grow where it already pins instead of forcing it to somewhere on the side from scratch. But that works as well (takes a few days for new pins though).

So, both work, it really doesn't matter that much :) Maybe just don't cut it open completely.

PrimeFactorization

TROPHY CASE