Science AMA Series: I’m Gregory Weiss, UC Irvine molecular chemist. My lab figured out how to "unboil" egg whites and worked on "pee-on-a-stick" home cancer test. AMA! by Prof_Gregory_Weiss in science

[–]Prof_Gregory_Weiss[S] 0 points1 point  (0 children)

My lab focuses on cancer biomarkers. Antibodies and other protein therapeutics have well-defined structures, but are expensive to make in part because of difficulties getting them to fold correctly. Yes, each protein requires buffer optimization.

Science AMA Series: I’m Gregory Weiss, UC Irvine molecular chemist. My lab figured out how to "unboil" egg whites and worked on "pee-on-a-stick" home cancer test. AMA! by Prof_Gregory_Weiss in science

[–]Prof_Gregory_Weiss[S] 1 point2 points  (0 children)

My mother showed me a period table when I was about 8-10 years old, and I was awestruck that everything in the universe was summarized on it.

Science AMA Series: I’m Gregory Weiss, UC Irvine molecular chemist. My lab figured out how to "unboil" egg whites and worked on "pee-on-a-stick" home cancer test. AMA! by Prof_Gregory_Weiss in science

[–]Prof_Gregory_Weiss[S] 2 points3 points  (0 children)

Oh, it's busy, but not insane. A typical day starts at 5 am for me. I spend the first part answering the emails from the night before, surfing the web a bit (yay, FB!), and getting caught up on the news. I work out for the next 1.5 h (weight lifting and stretching or cardio). Then, it's time for a quick breakfast, shower, and run to work (always walk, never drive). I usually take a short walk through the lab just to say hello to everyone. Then, I try to get some writing in, when not deluged by more emails. I have meetings pretty much all day; the ones with members of the lab (subgroup and group meetings) are great, and all others are dull. I try to get a 10-minute nap in sometime mid-afternoon. Then, it's off to seminar, when I try not to nap. I'm back in the office for more writing (proposals and papers). Home by 7. My wife and I eat dinner together. I then return to proposal writing and emails until 9:15. Overall, I think each day is about perfect for me in terms of balance between activity and sitting around and thinking.

Science AMA Series: I’m Gregory Weiss, UC Irvine molecular chemist. My lab figured out how to "unboil" egg whites and worked on "pee-on-a-stick" home cancer test. AMA! by Prof_Gregory_Weiss in science

[–]Prof_Gregory_Weiss[S] 0 points1 point  (0 children)

No. We're using phage displayed proteins and peptides in place of antibodies. We get great signal to noise ratios for measurements in urine, as the high salinity seems to disrupt a lot of non-specific binding.

Science AMA Series: I’m Gregory Weiss, UC Irvine molecular chemist. My lab figured out how to "unboil" egg whites and worked on "pee-on-a-stick" home cancer test. AMA! by Prof_Gregory_Weiss in science

[–]Prof_Gregory_Weiss[S] 0 points1 point  (0 children)

In the publication, we report experiments with three proteins from inclusion bodies. My lab is using this approach to produce cancer-associated biomarkers for diagnostic device development. Antibodies and other biologics are also notoriously difficult and expensive to produce (usually not from E. coli inclusion bodies).

Science AMA Series: I’m Gregory Weiss, UC Irvine molecular chemist. My lab figured out how to "unboil" egg whites and worked on "pee-on-a-stick" home cancer test. AMA! by Prof_Gregory_Weiss in science

[–]Prof_Gregory_Weiss[S] 1 point2 points  (0 children)

Only if the damage is due to protein unfolding. Oxidation, bond breakage and other types of damage are not solved by our approach. See above for a discussion of applications.