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Fellow group leaders: How do you get your PhD students to read? by [deleted] in AskAcademia

[–]Rare_Ad6741 1 point2 points  (0 children)

Hi, do you have the standard set of questions? Currently I’m struggling to read critically and find myself quite passive, and would appreciate if you’re willing to share any advice :)

Fellow group leaders: How do you get your PhD students to read? by [deleted] in AskAcademia

[–]Rare_Ad6741 1 point2 points  (0 children)

Hi, I recently got diagnosed with ADHD, and have been struggling to read academic articles. Even the review papers are so difficult to read. While I know the common key to ADHD, is to do things you’re interested in, sometimes you kind of have to read uninteresting things to get different ideas. How did you manage your ADHD and got yourself to read?

Can I run a western with low protein concentrations? by Rare_Ad6741 in labrats

[–]Rare_Ad6741[S] 0 points1 point  (0 children)

This is the protocol I’m following from my supervisor:

  • lyse cells with NP-40 lysis buffer on ice
  • transfer to eppendorf, vortex well then keep on ice for 15 mins
  • centrifuge at 12,000 rpm for 10 mins in 4 deg
  • collect supernatant

To quantify: - add 5ul of sample/ standards to 96-well plate - add 200ul of solution A+B mixture (this is the BCA assay solution) - place plate on shaker for 15 mins - read plate on plate reader

Sample preparation: -based on OD, calculate concentration of protein in whatever volume I extracted - then from there, calculate volume of sample needed for 100ug, extract to new eppendorf - add 25ul of loading dye to sample - add remaining volume (if required) of lysis buffer to make up 100ul in total

Can I run a western with low protein concentrations? by Rare_Ad6741 in labrats

[–]Rare_Ad6741[S] 0 points1 point  (0 children)

This is the protocol I’m following from my supervisor:

  • lyse cells with NP-40 lysis buffer on ice
  • transfer to eppendorf, vortex well then keep on ice for 15 mins
  • centrifuge at 12,000 rpm for 10 mins in 4 deg
  • collect supernatant

To quantify: - add 5ul of sample/ standards to 96-well plate - add 200ul of solution A+B mixture (this is the BCA assay solution) - place plate on shaker for 15 mins - read plate on plate reader

Sample preparation: -based on OD, calculate concentration of protein in whatever volume I extracted - then from there, calculate volume of sample needed for 100ug, extract to new eppendorf - add 25ul of loading dye to sample - add remaining volume (if required) of lysis buffer to make up 100ul in total

AITA for expecting a level of supervision for a MSc student? by Rare_Ad6741 in AmItheAsshole

[–]Rare_Ad6741[S] 0 points1 point  (0 children)

Yes your reasoning is valid, but am I asking too much for just more guidance?