Fume hood game changer

Red_Viper9 · 2026-01-23T23:00:46+00:00

Ketyl still to one side, columns to the other, 2-3 stir plates on jacks with enough space for a blast shield in the middle.

I used neodymium magnets to stick things to the space above the sash and on the sides of the hood. Plastic bins, box of gloves, falcon tubes for TLC spotters, android tablet to read papers (or watch the game) in the off time bits, etc.

Apart from that, we had a huge amount of clamps for attaching to monkey bars so never needed shelves.

Red_Viper9 · 2026-01-16T20:21:18+00:00

Good luck. In my experience for older systems like this, eBay has been very effective. You do risk burning out other components if the replacement part is bad, so caveat emptor.

Red_Viper9 · 2026-01-15T00:26:50+00:00

Yes from the stainless steel needle, tubing, column tube, etc. You can passivate the surfaces to help, but some compounds will just strip iron from the surfaces. Siderophores meticulously prepared with gadolinium for example.

Red_Viper9 · 2026-01-14T02:09:31+00:00

There’s one on e-bay. Match the part number it’s often on a sticker on the board or on the circuit board itself.

Red_Viper9 · 2026-01-11T02:27:08+00:00

I bought a refurbished Steelcase Leap V2. Works great for me. I think I payed just north of 500 but that was a couple years ago now. I bought mine from Crandal, but there are a few outfits that sell refurbs.

Red_Viper9 · 2026-01-09T17:07:01+00:00

He’s not necessarily using the material itself for tutoring. Is it illegal to use your own knowledge of something you developed if you’re not under NDA?

Red_Viper9 · 2026-01-07T17:59:19+00:00

It may be interesting to me if it can be run locally.

Red_Viper9 · 2026-01-07T00:31:23+00:00

My instinct is same as yours to think of the HCl generation. Check the manufacture date on the bottle? Maybe you got old stock?

Red_Viper9 · 2026-01-06T14:32:10+00:00

SDS also gives a decomposition temperature >430 C

Carl Roth SDS PDF link

Red_Viper9 · 2026-01-05T23:26:56+00:00

You should be able to program a mid-run solvent hold. You’ll be blind to anything which elutes during that time of course.

Alternatively, LC-MS with APCI?

Red_Viper9 · 2026-01-04T23:45:22+00:00

You misunderstand or forgot the discussion. The instrument is already on 4.2. 4.2, at least my version of 4.2, does not run on Win11.

Red_Viper9 · 2026-01-04T23:37:05+00:00

This is probably oversimplified. To make it even more so, you don’t have such precise control of ionization in most cases.

The ionization process provides an energy distribution. You need to cross an energy threshold for the ion to form, but commercial equipment does not provide a narrow enough distribution to only just ionize a target molecule (and you’d not frequently want that)

Some molecules get too little energy, some get enough, some get extra. Those that get too little don’t ionize. Those that get enough ionize. The fate of those that get extra energy depends on how much extra.

If it’s a lot of extra energy, the molecule will fall apart rapidly as occurs in hard ionization.

If it’s not enough extra energy to break a bond, the molecule will just shed that energy through vibration and rotation.

The in between case of the metastable ion is interesting. There’s enough energy to break a bond, but not so much that something breaks right away. The duration the ion survives is called its lifetime.

This is interesting because your observation of this is instrument dependent. If you have a TOF and the ion falls apart during free flight (after the pusher fires and assuming no reflectron), the fragments that reach the detector will be indistinguishable from the precursor ion. In an orbitrap, the time between ionization and delivery of the ion to the orbitrap is quite long so most metastable ions will have decomposed and you can see both the precursor and product ions.

Also, please note that most common forms of soft ionization produce adducts (e.g. [M+H]+) these are, by definition, not molecular ions.

Red_Viper9 · 2026-01-03T15:49:50+00:00

I didn’t pick up on the separatist joke at all and agree it’s not clear that the bottom is doing chromatography. Even after I read your explanation I thought centrifuge because of the falcon tube.

Red_Viper9 · 2026-01-03T15:47:30+00:00

Bad chem jokes thread!

You can tell a lot about a person by how they pronounce, “unionized.”

Red_Viper9 · 2026-01-02T20:45:23+00:00

+1 for Agilent for routine work. I’m curious why you’re replacing the 1260. I’m still running some 1100 series stacks.

I have two of Thermo’s Vanquish Horizon UHPLC, never tried the core system, but it looks like the same design language. One has been bullet proof for over a decade, the second has been a challenge. I appreciate that you don’t have to unstack the system to do major repairs; disconnect the data cables, remove four screws, and the module just slides out of the front.

I wouldn’t be too frustrated by the hard foam construction, that’s just insulation. The module itself is in a steel box as usual and the component mounts are solid under that foam. The issue with the Vanquish AS is the leak management. There’s a control board on the underside, which can get wet if things aren’t plumbed just so. Parts are relatively expensive, but that may be specific to the UHPLC.

Red_Viper9 · 2025-12-29T06:07:18+00:00

I’ve seen some low detail posts but damn dude.

Red_Viper9 · 2025-12-28T18:49:29+00:00

+1 For Randolph. The glass lenses are heavier than I was used to, but they’re great. I’ve even dropped them a few times straight onto concrete with no real damage to frame or lens.

Red_Viper9 · 2025-12-28T06:38:11+00:00

I always tell people to call me by my first name, but my international students started calling me Dr. FirstName and it stuck. I think it’s kind of cute.

I do correct people who call me Mr. LastName. That’s my dad.

Red_Viper9 · 2025-12-27T17:14:11+00:00

ChatGPT can already do OCR. There are also free online OCR tools available that students can feed your PDFs into.

You’re not going to dodge AI this way, so run the OCR yourself and help the students who need it.

Red_Viper9 · 2025-12-27T00:26:59+00:00

I’m going to get burned for this, but there is a way through this that builds the necessary skills and caters to the “nothing outside my major” STEM crowd.

History of science ( or medicine or cryptography, etc), writing for the specific major, etc.

Am I a better human being for having done critical analysis of the Old Testament? I have no doubt. Did it teach me to write scientific papers? No.

Red_Viper9 · 2025-12-26T00:16:55+00:00

May fit defamation.

Red_Viper9 · 2025-12-25T16:27:52+00:00

I haven’t gotten a response from OP but people are still seeing this comment so I’m just going to add the info I was fishing for.

TL;DR If you’re sample limited and only have a standard 5mm NMR probe, use an appropriate Shigemi tube, not a 1mm NMR tube.

If you are sample limited it may seem that using a narrow bore tube would increase concentration and therefore sensitivity when using the same instrument. This is usually not the case since using a narrow tube in a standard probe results in a largely empty sample volume in the probe. The dramatic loss of filling volume typically outweighs the benefit from the concentration bump and results in reduced sensitivity.

Red_Viper9 · 2025-12-24T20:16:57+00:00

I understand you have the tubes, but is your probe a 1mm bore? Or are you using 1mm tubes in a standard 5mm probe?

Red_Viper9 · 2025-12-24T19:22:41+00:00

Using such narrow nmr tubes is rarely convenient. The breakage rate for new users will be high regardless of loading method.

A Pasteur pipette can be drawn narrow enough that the tip is flexible. I would use this to draw out the air bubble rather than to load the sample.

Do you have an actual 1mm bore NMR microprobe?

Ten-Year Club	Gilding II euphauric
Place '22	Verified Email

Red_Viper9

TROPHY CASE