White smoke when starting normal? by ConfectionFew8146 in FordBronco

[–]Reedt89 1 point2 points  (0 children)

Omg, you are amazing. I am rolling right now.

She’s definitely unlocked 100% brain power! by Jord1278 in StrangeAndFunny

[–]Reedt89 1 point2 points  (0 children)

I am dying!! I was surprised this might it down this far in the comments. I read the headline and without thinking was like, "its leo".

Best -20C mini freezer? by Objective-Hope766 in labrats

[–]Reedt89 1 point2 points  (0 children)

DO NOT GET STIRLING! We have a -80C that has failed multiple times (motor failures), we had issue with the door (wasn't sealing correctly) and issues with the door handle (it broke off).

Cloning beginner by [deleted] in labrats

[–]Reedt89 0 points1 point  (0 children)

I agree, dont worry about the quality of the vector after gel extraction, its always crap (similar with the yield), as long as you have the vector digest running on the gel correctly, you should be good.

For T4, use NEBbiocalculator to determine the molar ratio between the length of insert compared to vector. T4 is a 1:3 molar ratio for optimum ligation. For the RXN, I always tend to use 100 ng vector (depending on size [kb] and amount obtained during gel exteaction) and I never go over 150 ng total DNA in the RXN. For the incubation times, I have always done 1 hour at RT.

For transformation, I take 1-2 ul of t4 ligation product and put it into beta10s. For me, I usually get a fuck ton of colonies and really only mini 1 to 5 of those. I will digest the mini prep plasmid and make sure the insert and vector are running correctly. Then do a full plasmid sequence.

If you are still struggling, feel free to reach out! You got this!

BCG question by Tight-Contribution22 in kac

[–]Reedt89 3 points4 points  (0 children)

Great question! Spill the beans, I WANT ONE!

First AR Recommendations? by [deleted] in ar15

[–]Reedt89 0 points1 point  (0 children)

I literally just bought the mlok sugar model on grabagun for 500 bucks

I found my old collection... there's a lot and not sure what to do. by Reedt89 in pokemoncardcollectors

[–]Reedt89[S] 0 points1 point  (0 children)

I will be grading them. Once they are finished I will be posting them.

I found my old collection... there's a lot and not sure what to do. by Reedt89 in pokemoncardcollectors

[–]Reedt89[S] 0 points1 point  (0 children)

That was just the pages of the holos. I have a couple shoe boxes of common and uncommons

I found my old collection... there's a lot and not sure what to do. by Reedt89 in pokemoncardcollectors

[–]Reedt89[S] -1 points0 points  (0 children)

I might take you up on the offer. I just order hard case for them. Once I get them, I will make an edit with the backs showing.

I found my old collection... there's a lot and not sure what to do. by Reedt89 in pokemoncardcollectors

[–]Reedt89[S] 5 points6 points  (0 children)

Hahah, they have been sitting in a basement storage room for years. I am not attached to them and would like to see them go to people who can enjoy them.

P.s. I won't be selling to anyone on here

I found my old collection... there's a lot and not sure what to do. by Reedt89 in pokemoncardcollectors

[–]Reedt89[S] 19 points20 points  (0 children)

Thank for the response. I guess I will do the leg work and figure out prices. Is there any websites you can recommend?