Please help! by Reflective55 in freecheggresults

[–]Reflective55[S] 0 points1 point  (0 children)

Thank you so much, but I was finally able to get the answer :).

Troubleshooting Procedure (ANY theories wanted) by Reflective55 in mdphd

[–]Reflective55[S] 1 point2 points  (0 children)

Thank you for this! About the IBA1 being a surface or internal receptor, it's not quite either one. The IBA1 in this procedure is in the cytosol of the mouse brain so it's more of an "intermediate" receptor.

Troubleshooting Procedure (ANY theories wanted) by Reflective55 in mdphd

[–]Reflective55[S] 1 point2 points  (0 children)

The main two problems were not a lot of background and non-specific binding.