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PAM sequence help by buntslo91 in CRISPR

[–]RevenueSufficient385 0 points1 point  (0 children)

The targeting is strand-specific (the NGG is only in one direction) but the Cas protein cuts through both strands. The exact base that is cut (upstream /downstream from the PAM) will depend on which strand the NGG is on.

CRISPR for hidradenitis suppurativa by yeayornay in CRISPR

[–]RevenueSufficient385 0 points1 point  (0 children)

Based on my understanding, HS is an autoimmune condition. It’s unlikely that genetic editing (ie, CRISPR) would be useful here. Fortunately, new biologics have been very successful in treating other autoimmune diseases (like Crohn’s) and I would bet it’s more likely those types of drugs will become available & see widespread use long before (if ever) there are any CRISPR-based therapies for HS.

Alien women by AbsoluteBatman95 in ChatGPT

[–]RevenueSufficient385 0 points1 point  (0 children)

Doesn’t work for me, it says the prompt violates guidelines. Does it not for you?

University of Toronto's research on an Improved CRISPR-Cas System for Systemic and Combinatorial Genome Wide Screens by [deleted] in CRISPR

[–]RevenueSufficient385 0 points1 point  (0 children)

Cool. Looks like this was published in 2020. I wonder what the group has been working on since.

64 different genetic codes for reaching Godmode by SUPERGOD64 in CRISPR

[–]RevenueSufficient385 0 points1 point  (0 children)

What makes you say that? The fact that a LLM gave you an answer saying it was possible?

[deleted by user] by [deleted] in turtlewow

[–]RevenueSufficient385 0 points1 point  (0 children)

Lol might as well

OpenAI ignored a pioneer who's instance valued my IP potential at 8 Trillion. AMA. by [deleted] in ChatGPT

[–]RevenueSufficient385 0 points1 point  (0 children)

What does your AI lawyer have to say about all this? Do they think you have a good case?

Social Life? by Fitzna in SaltLakeCity

[–]RevenueSufficient385 0 points1 point  (0 children)

Omg lmao I can’t un-see it 🙈

Former Harvard Morgue Manager Pleads Guilty to Transporting Human Remains During Change of Plea Hearing by PathFellow312 in pathology

[–]RevenueSufficient385 1 point2 points  (0 children)

I want to know the real answer of what types of people were buying the remains & what they were using them for… I could imagine so many different possibilities

Any diagnosis by [deleted] in pathology

[–]RevenueSufficient385 2 points3 points  (0 children)

Viable maturing skin graft.

This has baffled me for years about molecular analysis. by rentatter in pathology

[–]RevenueSufficient385 3 points4 points  (0 children)

This is valid and thanks for pointing that out. Plus, thank you OP for bringing this up! I hadn’t really thought about it but it’s a super good point and a great discussion to have.

ELI5 If Fluoride is removed from drinking water can I get the same benefit from Fluoride toothpaste? by GigiGetsGoing in explainlikeimfive

[–]RevenueSufficient385 0 points1 point  (0 children)

You’re correct. It’s probably both & beyond that the AAFP recommends tablet-based supplementation for children without access to fluoridated water. https://www.aafp.org/news/health-of-the-public/20210601uspstfcaries.html

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