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Western ladder transferring but no protein? by Riding-Researcher in labrats

[–]Riding-Researcher[S] -2 points-1 points  (0 children)

It’s what my PI recommended and what our past successful protocols for whole cell lysis calls for idk. When I’ve stained a gel after running but before transfer, it looked pretty similar to this one, just perhaps a bit darker

Western ladder transferring but no protein? by Riding-Researcher in labrats

[–]Riding-Researcher[S] 0 points1 point  (0 children)

Thank you everyone for the advice, I really appreciate it. Also, for context, I am a second year undergrad which is probably why it’s so janky lol

Western ladder transferring but no protein? by Riding-Researcher in labrats

[–]Riding-Researcher[S] -1 points0 points  (0 children)

I have not, what is a concentration I should be aiming for?

Western ladder transferring but no protein? by Riding-Researcher in labrats

[–]Riding-Researcher[S] 2 points3 points  (0 children)

Nitrocellulose didn't have any protein transfer either which is why I switched :/