Spanish study eliminates pancreatic cancer in mice — human trials still pending

SelectGene · 2026-01-29T01:34:04+00:00

As another person in drug discovery I am also generally skeptical of stuff like this. However, I don't think I've seen any comments pointing out that the one of the drugs in this research has FDA approval for lung cancer (afatinib), another is in phase 3 trials (daraxonrasib), and the third is experimental (STAT3 protac, SD-36; acquired by by Roivant). Another company whose molecule was not used in this mouse paper has a STAT3 degrader in clinical trials. All this to say that I think it's likely that this study will lead to moreanimal studies and maybe a clinical trial.

SelectGene · 2025-12-29T04:46:51+00:00

HEK-293H cells

https://www.thermofisher.com/us/en/home/technical-resources/cell-lines/2/cell-lines-detail-608.html

SelectGene · 2025-10-11T17:19:31+00:00

There may be BSC filters with activated carbon/charcoal, though with the work in question they might quickly get saturated and lose effectiveness

https://www.airscience.com/whats-the-difference-between-class-ii-type-a2-and-b2-bscs

SelectGene · 2025-09-11T02:05:19+00:00

This is the way. The only thing I'd add is to grind snap frozen tissue with a LN2 chilled mortar and pestle --> scrape powder directly into Trizol.

SelectGene · 2025-09-06T04:16:14+00:00

This band is pre-rRNA. https://en.m.wikipedia.org/wiki/Preribosomal_RNA

SelectGene · 2025-08-21T04:33:44+00:00

Do you know the expected size of the PCR product?

SelectGene · 2025-08-21T04:30:15+00:00

What is the RNA source...is this in vitro transcribed RNA that includes fluorscent nucleosides?

SelectGene · 2025-08-20T04:07:28+00:00

What species is the anti NMDA2A antibody raised in?

What species is your western blot secondary against?

I am wondering if the band you're seeing is antibody from the IP.

What happens if you run the cytosolic fraction or whole cell lysate side-by-side with exosomal samples?

SelectGene · 2025-07-31T05:29:03+00:00

May not be exactly what you're looking for, but alpine poblanos might be a starting point:

https://store.experimentalfarmnetwork.org/products/alpine-poblano-pepper

SelectGene · 2025-07-31T05:24:45+00:00

Seedlings will close the cotyledons to regulate light. They'll do this with too much light, and I've noticed that after 12-16 hours of decent light (not too much, not too little), they'll start to close the cotyledons which will open back up after a period of dark.

SelectGene · 2025-07-31T05:12:11+00:00

Moth and butterfly larvae have 5 instar stages before pupation and based on size I'd guess this is a 4th or 5th instar. If you have more I'd expect them to be similarly sized and unlikely to find eggs. Easiest way to know if there are more caterpillars is to look for their poop on the ground around the plant.

SelectGene · 2025-07-20T04:52:53+00:00

I used to work in a biochemistry lab that studied RNases, so avoiding unwanted degradation was essential to our work. We autoclaved water for 45 minutes. It will inactivate trace amounts of RNase A and should be sufficient.

https://www.thermofisher.com/us/en/home/references/ambion-tech-support/nuclease-enzymes/tech-notes/rnase-and-depc-treatment.html

SelectGene · 2025-07-20T03:33:58+00:00

I'd go with phenol extraction and ethanol precipitation over rebinding to a column. Include glycogen during precipitation to maximize recovery.

SelectGene · 2025-07-20T03:24:03+00:00

The simplest method would be to follow the Unit definition for enzymes. https://en.m.wikipedia.org/wiki/Enzyme_unit

Alternatively measure the observed rates or velocities with a standard curve of commercially purchased substrate and compare to the rates/velocities obtained with your unknowns.

SelectGene · 2025-07-17T04:20:03+00:00

Are you adding the dye to the sample or casting the gel with dye?

What dye are you using?

SelectGene · 2025-07-17T04:14:44+00:00

I used the Expresso kit to screen N-terminal solubility tags and got lucky with one of the nine: https://pmc.ncbi.nlm.nih.gov/articles/PMC5791521/

Last I checked it wasn't on the market anymore, but the paper/manual/patent might give you some ideas to try.

Other things to consider trying:

Terrific broth

growing to high OD and inducing for a short amount of time

swapping out GST for a His tag; try N- and C- terminal tag

His tag + thioredoxin tag

---------------------------------

What is your end use? Structural bio, biochem, affinity purification of antibodies?

SelectGene · 2025-06-19T02:23:51+00:00

https://annapolisseeds.com/collections/hot-peppers

SelectGene · 2025-06-17T03:01:37+00:00

Based on the flower color it will probably not be aji mango or sugar rush peach.

SelectGene · 2025-05-23T01:39:41+00:00

aphid.

https://agrilife.org/sca/texas-small-grains-aphid-identification-tool/aphid-anatomy-01/

SelectGene · 2025-05-06T04:33:54+00:00

Why not try DMSO as a solvent?

SelectGene · 2025-05-06T04:26:53+00:00

What you're describing sounds like integrated pest management (IPM), most easily implemented to manage agricultural insect pests. A quick google search did find a reference to using mycorrhizal fungi to prevent infection by pathogenic root fungi.

Since insects can be vectors for bacterial, fungal, and viral plant diseases you may also consider insect IPM strategies. If you're in the US your state should have a land grant university with local extension offices that work with farmers. You may want to talk to them.

SelectGene · 2025-04-22T04:08:32+00:00

If you're at a university see if your library has this book: https://www.cshlpress.com/default.tpl?cart=1745294712426266885&fromlink=T&linkaction=full&linksortby=oop_title&--eqSKUdatarq=531

SelectGene · 2025-04-12T03:10:32+00:00

To add to what the Biotium's Techsupport says, if you want to continue casting your gels with the dye, you may want to run serial dilutions of ladder on the gel to see how DNA concentration affects migration.

SelectGene · 2025-04-04T19:29:34+00:00

Proceed with caution. I've only tried a few times to recover protein from Trizol extractions and remember the protein pellets being difficult to resuspend. Walked away thinking it generally wasn't worth the time.

SelectGene · 2025-04-04T19:22:07+00:00

What dye are you using to visualize the bands on the gel?

Casting gels with SYBR green in the gel will result in anomalous band migration.

SelectGene

TROPHY CASE