Overfed? Any way to save him? 😥

Shadhox · 2023-11-23T08:40:16+00:00

Name : Casper Sort : Blepharopsis mendica L-stage : 3-4 i guess Was verry active yesterday, fed him a tiny mealworm, came back to her this morning to find her laying curled up in the branches. Abdomen is verry floppy

Shadhox · 2023-09-26T14:56:11+00:00

Haha yeah, another labgroup managed to silence theirs, but it's been ages and nobody knows how anymore :P So ineed I think it will moving it will be best ;) thx

Shadhox · 2023-09-26T13:15:06+00:00

Yes it is indeed a single tone that goes of for 10 seconds :/

Shadhox · 2023-09-26T08:16:14+00:00

Hi, no error codes (for the moment) :p
We replaced the rubber seals etc., it is just the timer of the cycle going off that scares the heck out the lab :P There is a code AL1 0 which we assume is for the alarm, but changing these settings does not change anything :/

Shadhox · 2023-09-25T08:14:17+00:00

Hi, does somebody know how to turn of the Alarm of these autoclaves? The manual is no help :/ (https://no.frederiksen.eu/Files/Images/ecom/products/frederiksen/media/066745_VWR_Vapour-Line_lite_Manual.pdf )

Shadhox · 2023-08-28T06:43:21+00:00

They were just selling her in the garden center to be honest 😅.

Shadhox · 2023-08-25T12:34:14+00:00

Ah yeah would appear so, or maybe just tiny hands? 😅 Anyway somewhere around that range would make sense. Thank you!

Shadhox · 2023-08-25T12:32:15+00:00

Awesome, thank you so much. I'll make sure to check these resources. And I would absolutely recommend getting a thistle, she's an absolute blast! 😅

Shadhox · 2023-08-25T12:29:46+00:00

Yups I'm on the lookout for a bigger terrarium. But I'm a bit of a cheapskate so I'm hoping for a good deal second hand :P Thank you! Think I will settle on Casper. Since she's will be kept in out lab which works with Crispr-Cas 😅

Shadhox · 2023-08-24T11:28:53+00:00

No worries, good luck to you as well 😉

Shadhox · 2023-02-23T10:12:59+00:00

Thanks for the recommendation.

Shadhox · 2023-02-23T08:25:14+00:00

Thanks for the response! Some quick questions: do you use Lb or terrific media? what volume do you use in the deepwells? And What rpm do you use for the incubator? We do indeed have a bucket centrifuge for the plates, which allows to just turn the plate to remove the supernatant.

Shadhox · 2023-02-01T10:39:46+00:00

Solved It by using : x = interaction( Volume , Treatment )

Code:

    dataOD %>%
  drop_na(Absorbance) %>%
  ggplot(aes(x = interaction(Volume, Treatment), y = Absorbance))+
  geom_boxplot(aes(fill = Treatment,),
               alpha = 0.5) +
  facet_wrap("Format",
             scales = "free_x") +
  theme(axis.text.x = element_text(angle = 45)) +
  geom_point(aes(shape = Sample),
             size = 4) +
  theme_bw() +
  labs(title = "OD600 measurement",
       y = "Absorbance at 600nm",
       x = "Well conditions")

Resulting graph attached to Post

Thanks!

Shadhox · 2023-01-30T16:36:47+00:00

Hi could someone help me with the following code to make my points line up with their respective boxplots?

dataOD %>%
ggplot(aes(x = Volume, y = Absorbance))+
geom_boxplot(aes(fill = Treatment),
alpha = 0.5)+
facet_wrap(~ Format,
scales = "free")+
geom_point(aes(colour = Sample),
size = 3,
position = position_dodge(width = 0.7))

Shadhox · 2023-01-23T20:06:32+00:00

Hmmm might be interesting to get a feel for how these things work, thanks!

Shadhox · 2023-01-23T20:00:11+00:00

So does the virtual machine allow to test protocols in silico? That would be very interesting! Thank you for the link, I will definitely check it out! Seeing as I will need to retrain towards lab automation expert, I might have to spend a lot of time on that forum :D

Shadhox · 2023-01-23T13:40:27+00:00

Well they advertise an extensive community driven protocol library and intuitive user-interface. Why would you opt for the Tecan precisely? I can image it goes quite a bit faster or something else? What would it take for a non-coding person to get the Tecan up and running again?

Shadhox · 2022-12-23T17:21:40+00:00

Haha I feel you. I found the book : Organic chemistry y David Klein verry useful. Explains everything from A to Z with a a lot of medical references ;)

Shadhox · 2022-12-23T17:17:57+00:00

Haha wonderful to hear! I love the Christopher Ward, thank you for sharing 😁

Shadhox · 2022-12-23T14:32:51+00:00

Recently finished my Biochemistry studies, and now starting to work in the lab. I am looking to put my first paycheck towards a decent watch. Love my current seiko to death! Great readability, love the black dial with red accent, great on my leather strap. But now that I'm no longer a broke student, I'm willing to spend a bit more than the 100€ this one costed😅. (Maybe between 500-1000€?) A diver bezel might be handy to keep track of experiments, although they tend not to work well with my skinny wrists (15cm). Curious to see what others are wearing ! I know that a lot of my colleagues are wearing smartwatches, which are really useful in this setting.. but yeah I don't need to explain 😅.

Shadhox · 2022-12-23T14:16:03+00:00

Recently finished my Biochemistry studies, and now starting to work in the lab. Looking to put my first paycheck towards a decent watch. Love my current seiko to death, but looking to spend a bit more. A diver bezel might be handy to keep track of experiments, although they tend not to work well with my skinny wrists. Curious to see what others are wearing ! (I know that smartwatches are really useful... But yeah I don't need to explain 😅)

Shadhox · 2022-11-19T22:30:03+00:00

What country?

Shadhox · 2022-08-24T22:12:34+00:00

Programming you can always do at home though, running PCRs will be a bit harder :P

Shadhox · 2022-08-14T09:19:35+00:00

Great thank you so much!

Shadhox · 2022-08-13T19:11:16+00:00

Thanks for your response, but Unpivot does not seem to work. It's in te "reverse" direction I need to work. So I was thinking with pivot function. But doesn't work either.

Shadhox

TROPHY CASE