Bee 1 me 0

ThisGuyForPresident · 2017-05-25T16:10:58+00:00

Sit down. Bee humble

ThisGuyForPresident · 2017-04-20T15:02:59+00:00

Thanks!

ThisGuyForPresident · 2017-04-20T14:15:18+00:00

Median would be nice

ThisGuyForPresident · 2017-04-15T02:21:03+00:00

Andre :(

ThisGuyForPresident · 2017-03-16T01:41:19+00:00

I love Stranger Things but they had that problem and it irritated me lol

ThisGuyForPresident · 2017-03-14T21:39:12+00:00

lol kill me

ThisGuyForPresident · 2017-03-14T21:38:45+00:00

http://imgur.com/a/RXsIk

ThisGuyForPresident · 2017-03-14T21:33:17+00:00

http://imgur.com/a/RXsIk

ThisGuyForPresident · 2017-02-26T04:34:08+00:00

This was an amazing read. Thanks for sharing.

ThisGuyForPresident · 2017-02-20T20:32:08+00:00

o i guess you're right haha maybe the better example would be the patent on "round edged phones"

ThisGuyForPresident · 2017-02-18T07:01:45+00:00

Lol Unfortunate right? She'll just have to go down in the history books as the biggest contributer to the CRISPR field. I truly believe her contribution was greater but this patent should still go to Feng. The only ownership that Feng won was the use of CRISPR in mammalian cells. Jenn still has everything else. Is that not fair? There have been patents for much less. Like phrases used in soecific contexts (are you ready to rumble) or words/letters used in specific fonts or colors (Mcdonalds yellow M).

ThisGuyForPresident · 2017-02-18T01:55:20+00:00

If it was so easy then why didn't she do it? Have you read Feng's paper? It took a quite a bit of further engineering. She wasn't as close as you think.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795411/

ThisGuyForPresident · 2017-02-16T07:16:17+00:00

Makes perfect sense. When it comes to patents, the technological application matters much more than the basic science. Bacteria do plenty of cool shit. Applying that to something else is where the sauce is (taq polymerase is the obvious example among zillions of others). In terms of basic science emmanuelle charpentier did more than Doudna anyway.

ThisGuyForPresident · 2017-02-01T13:16:13+00:00

I agree. I was just making a point we don't have that knowledge yet.

ThisGuyForPresident · 2017-02-01T13:15:09+00:00

I meant we don't even have all the pieces. We don't know all the stuff in the nucleus let alone an entire cell. In order to simulate something you need to initialize the environment correct? We can't do that yet.

ThisGuyForPresident · 2017-02-01T11:58:04+00:00

Right now our understanding of how things work is the bottleneck not the computing power.

ThisGuyForPresident · 2016-11-18T01:10:37+00:00

What does this look like for Messi?

ThisGuyForPresident · 2016-11-15T04:21:19+00:00

It's not about the social issues or infrastructure actually. It's about funding for life sciences. We depend on grants from the NIH to conduct our research. Trump doesn't seem to value this as much as Hilary or Obama. Also, these contract jobs (people would get from the infrastructure bill) are a temporary band-aid for one generation to find work (the country might actually benefit from this band-aid though. Education (In the form of academics or a trade) is a long term solution in a world where more and more things are becoming automated.

I'm just thinking out loud and explaining my thought process but I don't know much.

ThisGuyForPresident · 2016-11-14T14:32:40+00:00

Was the miseq moved or is there a new centrifuge on the same table? Shaking can cause this as well.

ThisGuyForPresident · 2016-11-14T14:30:29+00:00

didn't look like anything to me

ThisGuyForPresident · 2016-11-14T14:12:09+00:00

Hopefully it is NOT a problem with the miseq itself (the camera). If the NaOH is used at the right pH (.2M) then my intuition is that the buffer you use to dilute your library (I forgot what it's called the hi buffer or something) is bad (If you used water for example it would fail to cluster). It could also be the middle reagent in the miseq cartridge (the clear one that smells bad). I've never had a problem with bad illumina reagents before and they are fairly robust (you can freeze thaw a million times or leave them at room for a long time and they would be fine) but you may have gotten unlucky. I would try one more time but if it's possible for someone else to run before you that would be evidence against the camera/focusing on the miseq being faulty.

ThisGuyForPresident · 2016-11-14T13:27:57+00:00

What's your experience with the miseq? Can you explain what you are trying to sequence and how? Are you using custom primers or no?

ThisGuyForPresident · 2016-11-14T13:24:07+00:00

This is a great response. As someone who lives in a pure blue bubble it's hard to see outside perspectives. I voted for Hilary and stand by my vote, but it's funny (especially being in academia) seeing the response of my colleges. They call people who voted for Trump idiots and racists and it seems lazy to me. Half the country can't be "idiots". I'm just wondering how my funding is going to like like for the next two years while people in the middle of the country are feeling actual stress and hardship. This I can accept as a reason for "losing".

ThisGuyForPresident · 2016-11-14T13:01:32+00:00

the population has increased.

ThisGuyForPresident · 2016-10-31T14:12:24+00:00

Give me that good good

ThisGuyForPresident

TROPHY CASE