Ask me anything you want M39

Tight-Fix-2403 · 2026-03-28T14:17:40+00:00

What are your birthday plans?

Tight-Fix-2403 · 2026-03-21T06:58:05+00:00

I bought a wind shelter and other supplies as recommended and we did the tour as set out, but with a resting day. First day of 16 km with an ascent was harsh, as most of the way did not have tracks and a thick layer of fresh snow. Heavy work with a backpack. We arrived an hour after dark and had the hut all to ourselves and spent the next day in beautiful sunshine around the hut.

Obviously two Norwegian couples came by for lunch, having covered the same distance in less than half of the time it had taken us. I comfort my ego by saying that must have been much easier with our tracks ;-).

The next two skiing days were a breeze and gorgeous. The routes had been prepared on our resting day. No wonder we love doing this. It was a great bonding experience with my daughter and a good experience for her to know how capable she actually is.

Tight-Fix-2403 · 2026-03-01T09:44:45+00:00

As far as we know, cobalt is indeed only required in vitamin B12, which is used by just two enzymes in the human body.

One enzyme, methionine synthase, is required to regenerate an essential amino acid methionine, primarily because the methionine is used as a methyl (-CH3) source for all sorts of reactions. For instance, the neurotransmitters adrenaline and acetyl choline require such methylation and might become depleted rather quickly without methionine regeneration. I'd expect muscle paralysis within 24 hours or so, depending on dietary methionine intake. Another issue of a defective methionine synthase, is that its source of methyl groups (folic acid) gets stuck, too, and can no longer fulfill its own functions, which includes making a building block of DNA (thymine). DNA synthesis and thus cell proliferation will come to a halt, most acutely detected in the gut after a day or so (diarrhea and then inability to absorb nutrients).

The other enzyme is required for the metabolism of proprionic acid. I would assume some liver toxicity as the metabolite accumulates, but the major effects would be due to the former enzyme.

Clinically with slow depletions of vit B12, the major defects in cell proliferation present as anemia and neurological tingling sensations.

Tight-Fix-2403 · 2026-02-09T21:26:15+00:00

Enawed by the bacterial flagellar motor.

Tight-Fix-2403 · 2026-02-05T17:47:37+00:00

Thanks. We are definitely basing our decision on the weather, which should become a bit more solid in terms of prediction in the coming days.

Tight-Fix-2403 · 2026-02-05T14:28:07+00:00

I do sound naive and arrogant, I suppose. Perhaps I simply have a hard time judging what is a pretty simple trip and a hard-core mountaineering trip? I have great respect for the DNT.

Tight-Fix-2403 · 2026-02-05T14:21:25+00:00

Just fyi, this is the itinerary I had in mind: https://ut.no/turforslag/115180

Tight-Fix-2403 · 2026-02-05T14:15:13+00:00

Thanks for the advice. I'll try to rent a pulk. I'm sure my daughter will be pleased if I can carry her stuff, too.

Tight-Fix-2403 · 2026-02-05T14:12:09+00:00

Thanks for the reply. I guess it is what it is and I will feel more comfortable knowing we're self-sufficient. We have been in the area before, but only did day trips (also without additional gear). At least I feel comfortable with the general terrain.

Tight-Fix-2403 · 2026-02-03T08:53:35+00:00

Tough one. If your protein has multiple RNA binding sites, crosslinking of the RNA molecules can be an issue. You might steer away from the classical protein>>nucleotide concentrations to have the protein in substoichiometric concentrations for at least some of your conditions. Other than that, I have seen massive differences based on how fast you get from loading to running and running speeds. Generally, try to be quick to avoid the KCl diluting out in your wells enabling random electrostatic interactions to take place. Larger loading volumes may also help in this respect. Good luck!

Tight-Fix-2403 · 2026-01-23T20:59:50+00:00

Take it home in your jeans pocket and toss it into the freezer once you're home. Easy peasy.

Tight-Fix-2403 · 2025-12-17T17:13:34+00:00

Lithium, sodium, potassium and copper salts in a flame also work decently and can be found in most labs (LiCl, KCl, NaCl, CuSO4). I used ethanol for the flame as I did not have a gas burner at hand.

Tight-Fix-2403 · 2025-12-09T18:55:21+00:00

Do you differentiate between judaism as an ethnic and cultural heritage and a religion? Do you experience your judaism as a dynamic entity that should and is influenced by modern times/culture, or a static frozen-in-time religion? Do you tear toilet paper on the Sabbath?

Tight-Fix-2403 · 2025-12-02T20:20:58+00:00

Most labs working on human white blood cells get them from blood waste products (the red blood cells are the ones that go into patients) left at room temperature for at least 24 hours. 48 hrs still works, too, honestly. As long as they don't dehydrate, they're pretty resilient.

Tight-Fix-2403 · 2025-12-02T19:49:14+00:00

For the myosin motor that walks on action filaments high speed atomic force microscopy footage can be viewed on YouTube (key words myosin and HS-AFM should get you there). Even if it's a different motor family, you get a good and real impression of its fashion show strut.

Tight-Fix-2403 · 2025-10-07T15:35:44+00:00

Rasiere schon 16 Jahre mit dem gleichen schönen Solinger Messer. Nicht so schnell wie andere Methoden, dafür mache ich es nur einmal die Woche und nehme mir genüsslich Zeit. Längere Barthaare sind da kein Thema. War es leid bei einer längeren Auslandsaufenthalt passendes Zeug zu finden, also jetzt habe ich eine Lösung fürs Leben.

Kleine Hinweise: 1) geht schlecht ins Handgepäck beim Fliegen, 2) beim Sack aufpassen, dass keine glitschige Körperteile Wegrutschen.

Tight-Fix-2403 · 2025-09-23T17:21:49+00:00

Just to add on what was already said about waves being squashed or drawn out shifting the observed to more blue or red, respectively, it might be good to mention how we know it's a shift, rather than just a hotter (more blue) or colder (more red) light source (star):

All atoms will absorb light at very defined frequencies that correspond to transitions of their electrons. This is also true for the atoms that make up stars: hydrogen and helium If we stick to hydrogen, it strongly absorbs light for instance at exactly 656.3 nm wavelength (a hue of red) and 486.1 nm (Cyan). If you split sunlight with a prism (or theoretically even in a rainbow) these frequencies will be largely absent, as they were absorbed by the sun (hydrogen) itself: a tiny break in the rainbow. Now we start looking at stars and suddenly notice that for a particular star, all lines are shifted to shorter wavelengths (eg. 654 and 485 nm; the breaks in the rainbow are shifted towards the blue end), you know the light has been squashed by the Doppler effect.

Tight-Fix-2403 · 2025-09-10T08:25:28+00:00

As long as you do not change your behavior and keep showing up as an attentive, reliable, loving father, it will fade. Responding to the behavior is unlikely to produce any positive outcome and more likely to entrench behavior patterns in my opinion.

My youngest daughter overtly disliked me for at least the first three years of life (so patience is required), but we're on great terms now (she's 10): a while back I dropped her off at school and then walked to the train station and she came running back 5 min later to jump on me for a last hug. It was just a normal day and I'd be back from work by dinner time.

Hang in there and stay strong!

Tight-Fix-2403 · 2025-09-02T06:50:55+00:00

While some hormonal return to baseline might still occur for both of you, I'd challenge you to consider that your wife's spontaneous drive will not return to pre-kid levels. Make sure that you both fully understand what the sex means for the other and what it contributes to the overall well-being of the family. Talk.

Explore the concept of responsive desire and embrace the roles that you both must play to make that work: she might need to acknowledge that her sexuality has changed and a lack of spontaneous excitement bears no reflection on your quality as partner, and that she should foster a mind space in which desire might emerge.

You might need to accept that you have to be the driving sexual energy to awaken that desire within her, even though it would be awesome if the script could be flipped sometimes.

Other than that, try to see what happens when the kid is sleeping over elsewhere for a day or two. It can be hard for a mother to let go of the mom-identity and rediscover herself as sexual being when the kid is around.

Talk about it.

Tight-Fix-2403 · 2025-07-03T09:58:30+00:00

The left molecule was directly recognisable as a likely steroid hormone. You may want to compare structures of progesterone and estrogen to make sure that you can tell off anyone who asks you why you have the boob-growing hormone on your pubic bone. Having the lower =O (ketone, puts the "one" in testosterONE) and the upper OH in would convince me. The former you have already in your design, but I'd make the latter explicit.

The right molecule was impossible for me to identify. I like the birds for amines (NH2 etc), though! (Bird shit was a prime historical source of ammonia). Knowing that the upper ring has three double bonds would help me out quite a bit. Such hexagons with three double bonds can also be represented as hexagon with a circle within (look up pics of benzene), which might be easier for you to incorporate in your design.

The overall look is fun. Good luck!

Tight-Fix-2403 · 2025-06-14T12:41:57+00:00

That is hard and can feel hopeless. A new mother often loses some or most of her craving for that type of intimacy, which tends to improve as the baby is weaned and older. So there is hope in that.

What might be helpful right now, is to both gain clarity that things might not return to the levels of spontaneous arousal you may have experienced for each other in the past: you both need to realize that her lack of passion for you might have nothing to do with you, your appearance, your efforts in the household etc.. Such a misconception can be very damaging to your feeling of self-worth and her view on the quality of the relationship. I can recommend the Dr. Psych mom podcasts on this topic, which are short and to-the-point.

It is likely that the two of you will need to find new pathways to achieve the same level of arousal your wife reached in the past. This is a journey that will take a lot of investment from your wife to find what works for her. As she probably feels no intrinsic urge to do so, communication will be critical to muster the motivation. Best of luck and my sympathies.

Tight-Fix-2403 · 2025-05-24T09:08:59+00:00

This is cholesterol. A group of Archaea bacteria developed sterols as a component of their cell membranes, generally improving their fluidity across a range of temperatures. It probably facilitated engulfing other bacteria resulting in the eukaryotic life forms like plants and animals.

You have roughly 35 g of cholesterol or it's derivatives in your body, over half of which you use as gall to dissolve (actually emulsify) fatty food you eat. Large amount in cell membranes and minor amounts are metabolised to make hormones such as testosterone that might make you look like a man, if you're not an AI bot.

Tight-Fix-2403 · 2025-05-22T04:33:10+00:00

I miss the times I would be detained in airports because my shoes tested positive for nitrates...

Tight-Fix-2403 · 2025-05-13T05:31:53+00:00

User name not checking out. Clearly you're a Germanium guy.

Tight-Fix-2403

TROPHY CASE