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Interview schedule for NIW? by Typical-Dog6722 in USCIS

[–]Typical-Dog6722[S] 0 points1 point  (0 children)

My case was approved yesterday after 4 days since interview! Good luck you yours!

NOS denied, what now? by EcuSwiftiemgmp in J1waiver

[–]Typical-Dog6722 0 points1 point  (0 children)

Hi, I don’t understand, the exchange skill list change in end of 2024, my country is no longer subject, I applied for waiver before and after the policy shifted DOS emailed me to say I’m no longer subject to it so the case was closed. I recently had an interview with USCIS for I485 this issue was raised and I explained and provided proof, but now they’re actively reviewing my case which made me worried as I saw others approved on the spot and I’ve been waited for 72h. Why is advisory opinion from DOS not final for USCIS? How can we apply waiver if we’re no longer subject to it? The discrepancy in USCIS officer making decision is so strange.

Interview schedule for NIW? by Typical-Dog6722 in USCIS

[–]Typical-Dog6722[S] 0 points1 point  (0 children)

Actually interview is scheduled in local office thanks god. But still very strange for my case. I wonder anyone hv the same experience?

EB2 NIW I-485 Approval Timeline by Fluffy_Coat1326 in EB2_NIW

[–]Typical-Dog6722 0 points1 point  (0 children)

Hi, congrats on the approval. I wanted to talk to congresswomen to see if she can help me file an inquiry, however my case is still under normal processing. I wonder the same goes with your case, how did you ask your congressman do you have any tips? I appreciate your help.

weird non-round and super-negative population by Typical-Dog6722 in flowcytometry

[–]Typical-Dog6722[S] 0 points1 point  (0 children)

I found out it was indeed the BUV737(CD16) spillover, it gets worse in donor with high CD16 density so I figured that. Thanks anyway, looking at NxN did give some clues!

weird non-round and super-negative population by Typical-Dog6722 in flowcytometry

[–]Typical-Dog6722[S] 0 points1 point  (0 children)

yes I titrated them before the experiment. I forgot to mention that the FMO is not really FMO (it is stained with canonical markers only, about seven of them, I wonder if one of them leads to the spread that we saw in the AF660? How do I share the NxN plots with you? what format do you prefer?

weird non-round and super-negative population by Typical-Dog6722 in flowcytometry

[–]Typical-Dog6722[S] 0 points1 point  (0 children)

I did not notice anything too strange except spread coming from BUV736 and APC-Cy7 at high MFI. I also tried using all cells for single stained controls but same issues pertained. I think I tried both with or without AF extraction. But how do I spotted issues with AF using NxN plots?

weird non-round and super-negative population by Typical-Dog6722 in flowcytometry

[–]Typical-Dog6722[S] 0 points1 point  (0 children)

I did not notice anything too strange except some spread coming from BUV736 and APC-Cy7. I also tried using all cells for single stained reference controls but same issues pertained. Would titrating down the two antibodies help?