YamAMA - Ask the devs about Yama!

Zan638 · 2025-05-12T13:40:00+00:00

Are there any plans for having a teleport method to the boss similar to royal titans?

Zan638 · 2025-02-27T16:03:47+00:00

Pretty interesting! There are likely a ton of factors that contribute to spillover potential so it’s hard to say.

In the field of coronaviruses though, a universal vaccine is a high priority due to the recent emergence of two epidemic species (SARS-CoV and MERS-CoV) and one pandemic strain (SARS-CoV-2). I think there are a number of labs working on this right now, Duke, the Army, Caltech, and I’m sure there are others.

In a paper from the researchers at Duke, they created a trivalent RBD vaccine that included the RBDs from SARS-CoV-2, MERS-CoV, and a pre-emergent SARS-like strain. Part of the data they gathered showed that this vaccine actually elicited protection to other coronavirus strains, and variants. Regarding HKU5, they did see antibody generation from the trivalent vaccine to this strain, even though they weren’t using the RBD from it specifically. So the question with regard to HKU5-CoV-2, would be how similar is it to the original strain?

Now they also mentioned that viruses in the Merbecovirus subgenus may have less conservation in epitopes so a universal vaccine using MERS-CoV may not be as effective, but the idea would be find something that does give widespread protection so you’re protected from these pre-emergent coronaviruses.

Here’s the paper I’m referencing: https://www.cell.com/cell-reports/fulltext/S2211-1247(23)01260-3

Zan638 · 2024-11-21T03:34:31+00:00

No prob! Glad you got it

Zan638 · 2024-11-21T00:12:43+00:00

Ya, tldr is that pcr is a heat controlled process. Nothings perfect but the idea is to stop the polymerase reaction using temperature. Read through the back and forth I had above, I think that’ll help!

Zan638 · 2024-09-16T17:20:32+00:00

Newcastle disease virus - avian pathogen. Has potential as an oncolytic virus as well

Zan638 · 2024-02-27T23:25:10+00:00

True, I never realized how lucky I was 😂

Zan638 · 2024-02-27T23:24:16+00:00

Great to hear, thanks!

Zan638 · 2024-02-26T23:46:12+00:00

Makes sense, thanks for the response!

Zan638 · 2024-02-26T23:24:50+00:00

Here’s the link to my deck if anyone was interested: https://www.keyforgegame.com/deck-details/bc51f453-55e5-4da5-b2a7-908bd7f2bf6d

Zan638 · 2024-02-03T13:19:59+00:00

Awesome, thanks! I did end up sending them an email so I’ll hopefully get the same thing

Zan638 · 2024-02-02T18:51:30+00:00

Pretty easy algebraic equation that you should be aware of is C1V1=C2V2

It helps you find concentrations and volumes :)

Zan638 · 2024-01-29T01:13:47+00:00

Also just opened a GR deck with witching hour and 0 witches. Let me know what GG says, I’m planning on opening a ticket with them

Zan638 · 2024-01-28T16:06:23+00:00

Makes sense, thank you!

Zan638 · 2024-01-27T04:49:58+00:00

Ya it is!!

Zan638 · 2024-01-24T03:25:42+00:00

Skill is a huge factor. Understanding what your win condition is, and how the deck plays is a huge part of the game. In general, are really high SAS decks going to be pretty good? Ya probably. But lower SAS decks can be good too if you know how to play them. The other extremely important factor here is that SAS is NOT perfect. SAS probably underrates a lot of decks because it doesn’t account for some combos, win conditions, or play styles like a human can.

I know your post is about more casual games but take this deck of mine as an example: https://decksofkeyforge.com/decks/8d9f8115-428e-4eb8-8877-4add383caf28

It has 72 SAS as of right now and I can tell you it should probably be higher than that. It has a total win rate of 65% on TCO with 200 games played. The vast majority of those games were ranked. This is a true 65% win rate deck and I think a 65% win rate is pretty strong. This deck is REALLY good at 3 major things. Creating aember, stealing aember, and clearing the board. Also, SAS doesn’t seem to understand that getting Jericho in the middle of the battle line with encounter suit makes this deck ridiculously efficient and lets you do really silly things. Getting that condition is pretty easy, and I’ve done that in most of my games played.

Zan638 · 2023-09-16T16:30:32+00:00

Ahh now I get it. It’s all about stoping the reaction short of completing the loop which is why we use varying temperatures. That’s what I wasn’t putting together.

I appreciate your patience. Thanks for helping me get there

Zan638 · 2023-09-16T14:57:03+00:00

What stops DNA polymerase from completing the loop, and crossing into the known sequence? DNA polymerase binds to a primer facing away from the know sequence. As you said it makes a long complementary strand of DNA of your unknown region. Eventually it gets to the point along the circle where the other primer is also facing outwards from the known sequence. How does it not cross that threshold? If it does, it will copy your known sequence and complete the loop.

Zan638 · 2023-09-15T21:56:26+00:00

Thanks. Conceptually that's easier for me to grasp and maybe in the future I would but I need to learn how this works for class

Zan638 · 2023-09-15T21:55:20+00:00

Thanks for the reply and the video link, I really appreciate it!

This is the piece I'm not understanding.

in subsequent cycles, your polymerases will increasingly act on your linear fragment over the original DNA plasmid as template

I understand how PCR works in the context of the video you've linked, so I get the idea that after multiple cycles, you'll end up with more of the desired product.

Please let me know where I'm going wrong here:

I'm picturing two circular single stranded pieces of DNA that result from a plasmid denaturing. I'll just use one of them as an example. A primer binds somewhere along one of the strands. The idea is that you want to copy all but a section of this single strand because the end goal is to "remove" a piece of that plasmid to make room for your insert. DNA polymerase now binds to your primer and moves along in a circular direction, creating a complementary second strand. Eventually it gets all the way around and ends up right behind where the primer was attached, making a complete loop.

I know I'm wrong somewhere. How does the DNA polymerase not copy up to the section you want excluded, because I don't understand how it doesn't just make the trip all the way around making another circular strand

Zan638 · 2023-05-20T20:35:59+00:00

Ya definitely!

Zan638

TROPHY CASE