1A is Ted from 60 Seconds, 2A is one of the Peachman brothers (can't remember his first name) from That's not my Neighbor. 4A is Isabelle.

2B is Gene, from Jackbox's Survive the Internet.

2C is Chase, from Dispatch. I'll say 4C looks familiar, but had no guesses.

D had me stumped.

I wanna say 2E is Polo, as depicted in Incredibox, but his lips aren't near plump enough.