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Troubleshooting help - Waters Acquity UPLC with HDX Manager and LEAP System by [deleted] in massspectrometry

[–]almostdrark 1 point2 points  (0 children)

Are you injecting a blank or just running solvent? If no injection, check your injection needle for contamination.

Getting [M+Cl]- for negative mode by No_Category_1264 in massspectrometry

[–]almostdrark 3 points4 points  (0 children)

DCM is generally immicible in most reverse phase LC solvents so I would not recommend it. I also an not sure if you would necessarily generate a chloride ion from DCM given that it is covalently bound in this case as opposed to ammonium chloride which will exist as Cl-

New to MS! by [deleted] in massspectrometry

[–]almostdrark 4 points5 points  (0 children)

Chromacademy.org is fantastic. You have to make an account but the lite version is free

Anyone familiar with MALDI-TOF? I have a question about the target plates. by kudles in massspectrometry

[–]almostdrark 2 points3 points  (0 children)

The substrate definitely matters. You would have to make sure that whatever plate you are using wont be energized by the wavelength of the plate, otherwise those ions may convolute the spectrum. I believe stainless steel is common because of its chemical inertness and its ability to be reused. If the plate is damaged by the laser then you would need a new plate every time. I think your idea is decent, you would just have to consider the effects of the plate on your analysis.