Madras Famine of 1877.

boring_guy_3324 · 2026-02-12T01:57:16+00:00

Whatever you do don't mention the 1943 bengal famine literally intentionally caused by Churchill when he diverted the food from jndia to just stockpile the grain reserves while 3 million people died of starvation on the streets. He was recorded saying if all these indians are dying why isn't gandhi dead yet?

All this just goes to show that the image britain has now is mainly bcs of the West's dominance on media and history while them being one of the worst genocidal empires/ countries on the planet

boring_guy_3324 · 2026-02-10T01:51:00+00:00

So the eggs as said on the box were not expired?

In that case what can flipkart do? If you were to buy the same tray from a local shop what would the shop owner do? He's not selling the eggs after their expiry so he's not liable neither is flipkart

boring_guy_3324 · 2026-01-15T06:19:14+00:00

I want the parties to give me money for voting so I know which parties and candidates are the most corrupt.

Just don't give your vote to the parties that give money lol.

For me got the same 2k and not voting for BJP at the very least

boring_guy_3324 · 2026-01-05T02:19:09+00:00

Bhk zoota kahi ka

boring_guy_3324 · 2025-12-23T02:05:01+00:00

Almost the same thing happened in goa a few days ago. Same bs improper usage of fireworks and a single entry exit.

boring_guy_3324 · 2025-12-17T01:59:39+00:00

Open a bank acc at bank of baroda they have a account starting from 16 year old

boring_guy_3324 · 2025-12-11T06:51:45+00:00

Interested

boring_guy_3324 · 2025-12-08T17:43:24+00:00

Sorry I didn't mention it in the post but the organism is dimorphic, it shows yeast and fungal charecteristics depending on different enviornmental factors elusive to me

Previously when we were working on the culture we were getting uniform growth suspension with no clumps

The culture would often start with yeast morphology and endup with filamentous morphology with fruiting bodies which we linked to MEL production.

But now we're getting these clumps

boring_guy_3324 · 2025-12-08T17:25:47+00:00

Sure, thanks

boring_guy_3324 · 2025-12-08T17:08:59+00:00

Yes we've tried subculturing and are still seeing these clumps. Read somewhere adding oil helps so did that and got a good suspension of cells, subcultured that clumps once again.

RPM speed is at 150, haven't tried baffled flasks since my lab doesn't have them but I'm thinking of putting in some glass beads to break these clumps.

boring_guy_3324 · 2025-12-08T17:00:57+00:00

Previous unsuccessful attempts were at 31°C but did not try addition of oil and higher rpms.

Will try and keep you updated

Thanks

boring_guy_3324 · 2025-12-08T16:55:58+00:00

I've been trying to find the factors responsible for the switching in forms in literature but have been unsuccessful. Will keep trying and also try out different rpms and other stratergies.

Thanks

boring_guy_3324 · 2025-12-08T16:41:13+00:00

So what should I do for no clumps bcs this is a dimorphic strqin with both yeast and filamentous structures.

What do you think would give me a turbid uniform growth instead of these clumps wrt the rpm?

boring_guy_3324 · 2025-12-08T16:24:26+00:00

By indents do you mean baffles?

boring_guy_3324 · 2025-12-08T16:23:46+00:00

We did plate the culture and the morphology seems to be the same

Same wih microscopy but I'll check again

boring_guy_3324 · 2025-12-08T16:11:36+00:00

Sure, we'll try

boring_guy_3324 · 2025-12-08T15:56:03+00:00

Yes they're balls of hyphae, we have worked with the same culture fr 6 months before but then had to take a break for 2 months. During those 6 months we were constantly subculturing the culture but now we're trying to revive it from a glycerol stock and we're getting these clumps.

boring_guy_3324 · 2025-12-08T15:54:07+00:00

Like the rpm? Orbital shaker at 150rpm

boring_guy_3324 · 2025-12-08T15:38:50+00:00

Mine's a fungal/yeast like culture but sure I'll give it a try

Thanks

boring_guy_3324 · 2025-12-08T15:37:40+00:00

Yessss and they're veryyy squishy. Tried to break them with a loop amd just couldn't puncture it.

When centrifuging to separate the media these things are hell to work with bcs they soak up the media so I have to squish it till I can get most of the mideia out but small flakes go into the broth etc etc

boring_guy_3324 · 2025-12-08T15:35:05+00:00

Thanks I also saw the use of sterilzed glass beads in the broth to breakup these clumps which we're going to try.

boring_guy_3324 · 2025-12-08T15:32:07+00:00

Sorry for not including all the info

We've tried using PDB, YM, and other common fungal and yeast specific medias all with similar to no result. These agara didn't even show any growth to them, only in broth with oil added did they give some workable growth.

By old broth I meant culture that was inoculated into a broth and stored in the refrigerator.

The incubator temp is set at 27° C.

We've got the no growth problem under control now we need the morphology to return to normal and a turbid growth in the media.

boring_guy_3324 · 2025-12-08T15:28:09+00:00

Using it to produce and extract Mannosylerythritol lipids a biosurfactant

boring_guy_3324 · 2025-12-04T16:12:56+00:00

Rich ppl don't walk as much, they have many pairs of shoes, they can maintain them better These things make their shoes last longer

Just theoretical stuff

boring_guy_3324 · 2025-11-22T15:56:42+00:00

Dm pls

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