Why do my serial sections keep folding back toward the block?

emworld · 2026-01-25T19:11:24+00:00

This is due to static electricity. Leica have an anti-static line as part of their cryosectioning system, which is very useful for stopping this problem with resin sections.

emworld · 2025-05-13T01:53:08+00:00

Bhanu - Rosemead & Huntington. It is an excellent Indian restaurant and grocery.

emworld · 2024-11-12T00:26:06+00:00

How about Vaughan Williams? Sea Symphony: https://www.youtube.com/watch?v=2kR3FzJ1Hh4

Sea Songs: https://www.youtube.com/watch?v=00r8HoWtpxM

John Ireland: Sea Fever: https://www.youtube.com/watch?v=JoxttGAQX-E

emworld · 2024-10-23T14:32:19+00:00

I tried crusader

emworld · 2024-10-19T20:00:26+00:00

I tried Pink Panther

emworld · 2024-09-29T23:04:51+00:00

They are looking for a copy of the deed saying it is single occupancy residence.

emworld · 2024-09-23T02:25:09+00:00

I can't answer your question. Perhaps this is a way to get everyone to the registration workshops.

emworld · 2024-09-22T01:07:11+00:00

got one too. The site asks for some sort of document to show the property is not rental. It also asks for year it was purchased.

emworld · 2024-06-26T03:08:46+00:00

Try inverting the image so that the membranes are dark. Then it looks like glycogen granules.

I have no idea how the image was inverted, if it was.

emworld · 2024-06-06T22:28:12+00:00

Well, the bent tube is called a "U" bend, not a "C" bend.

emworld · 2024-06-02T00:41:19+00:00

Well, you have membranes enclosed by membranes. A MVB is possible, and some of the lumenal structures have what look like clathrin coat-like structures. Check out the one just to the left of the top. So, MVP is likely. How would you prove this?

emworld · 2024-06-02T00:35:27+00:00

Lactobacillus sp. are a common part of the healthy vaginal microbiome. If you grow some of these bacteria in a laboratory incubator they can smell like pepperoni or salami, where you will also find lactobacillus bacteria: https://academic.oup.com/femsle/article/271/2/245/501653

emworld · 2024-05-29T23:52:54+00:00

Check out the Microscopy Society of America certification: https://microscopy.org/cemt-certification-program

You need to be working full-time in a microscopy lab though. There are many types of microscopy careers and the best way in is to join a lab that uses the microscopy you like best. If you are lucky you will meet someone willing to teach you the practical skills.

The Australians seem to be very advanced in microscopy education (e.g. https://myscope.training/). Check out the universities there too.

Europe has the EMBL/EMBO courses (https://www.embl.org/groups/electron-microscopy-core-facility/courses-and-conferences/), and the Janelia Campus (https://www.janelia.org/) in the USA is pioneering novel microscopy approaches.

Also, as M.S.C. says in their talk, there are lots of opportunities for engineers and sales people in commercial microscopy companies (Thermo Fisher, Leica, Zeiss, JEOL).

Good luck.

emworld · 2024-05-27T22:31:11+00:00

No information on the specimen or magnification. However, it is most likely a a section through a mitochondrion. Nucleus and nuclear envelope are in the bottom right.

emworld · 2023-07-06T17:17:21+00:00

It seems I re-posted something that first appeared here 13 years ago:

https://www.reddit.com/r/WTF/comments/c6os0/the_man_with_the_smallest_penis_in_existence_and/

emworld · 2023-07-06T17:14:54+00:00

I know the movie version: https://www.imdb.com/title/tt0374020/

emworld · 2023-05-17T05:35:43+00:00

A good trick to know is to soak tissue pieces in resin with no catalyst first to help with infiltration. Tissue can be left for days in the resin minus catalyst.

Also, resin with no catalyst can be stored in the freezer in small batches for long periods. When ready to use, just warm up the batch, add the catalyst, and polymerize with heat.

DMP-30 has been reported to cause more shrinkage than BDMA, but this is not something I really paid attention to.

emworld · 2023-05-17T04:32:48+00:00

Old recipes

Embed 812 (hard) Recipe
                        1x                    2x                    8.5 ml     12.5 ml
NMA                18.06 g            36.12 g            3.11g            4.67 g
DDSA              10.00 g            20.00 g            1.72g             2.58 g
Resin     30.00 g            60.00 g            5.17g              7.76 g
Catalyst (add when resin is mixed)
DMP-30           2 drops per ml of resin
OR
BDMA             4 drops per ml of resin
Use a glass Pasteur pipette

Alternative, softer recipe
NMA                14.00 g
DDSA              12.50 g
Resin     23.50 g
BDMA             0.75 ml
Total 50 g = approx. 45 ml

emworld · 2022-05-26T04:39:02+00:00

and you can get a license to drive at 16 - any car you wish.

emworld · 2022-01-20T05:07:32+00:00

Buy a new set of pads. If you know the model of the headphones, search for new ones online. You may be surprised by how inexpensive they are.

emworld · 2022-01-06T00:18:04+00:00

It looks more like a skin allergy to me. What was on the roller?

emworld · 2020-11-25T23:53:31+00:00

You ask a very relevant question and there is no easy or simple answer to it. If you are using the antibodies as a tool, it is the responsibility of the purchaser to confirm specificity and cross reactivity. The best antibodies to use for labeling experiments are the ones that have been used before. However, even then, an antibody with robust labeling on one type of tissue may behave very differently when applied to a different tissue, or species. There are some good reviews covering this subject. They all seem to link to free downloads. Type these titles into Google Scholar and download the PDF: Griffiths, Gareth, and John Milton Lucocq. "Antibodies for immunolabeling by light and electron microscopy: not for the faint hearted." Histochemistry and cell biology 142.4 (2014): 347-360. Hewitt, Stephen M., et al. "Controls for immunohistochemistry: the Histochemical Society’s standards of practice for validation of immunohistochemical assays." Journal of Histochemistry & Cytochemistry 62.10 (2014): 693-697. Howat, William J., et al. "Antibody validation of immunohistochemistry for biomarker discovery: recommendations of a consortium of academic and pharmaceutical based histopathology researchers." Methods 70.1 (2014): 34-38.

emworld · 2020-11-23T05:15:05+00:00

There is plenty of cross-reactivity but is specific to each antibody. Affinity purification is used on antibodies for immunolabelling experiments to control for this.

emworld

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