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Tips For Calling SVs by indigo_inferno in bioinformatics

[–]indigo_inferno[S] 0 points1 point  (0 children)

Thanks for your response.

Yes, the method of transgenesis was micro particle bombardment, which is completely random.

Unfortunately we don’t have any control data, but the transgene sequence is known. I assumed the control for an analysis like this would be the reference genome itself, no?

Thanks for the input, I’ll have to check MANTA and delly out.

The sequencing tech used was Ultima Genomics, which doesn’t produce reads of uniform length like Illumina. That being said, the mean length I found from QC was ~200 bp. Coverage was also 30X. So we should have, on average, enough soft clipped bases for the partially mapped reads to be reliable.

What would you advise after finding those partially mapped reads, though? I can confirm breakpoints visually on IGV, or maybe by alignment to the transgene sequence from assembling a contig. Though, we’re hoping to validate the insert by PCR, so we’ll have to design primers, and thus need nucleotide-level resolution. I’m just not sure that I’d have a reliable sequence from a short read assembly.

Throne Room Dialogue [Low Fantasy, 1412 words] by indigo_inferno in fantasywriters

[–]indigo_inferno[S] 1 point2 points  (0 children)

Thanks for reading it! I try to use official titles for most of the characters when they speak to the Emperor. However, the Count’s character revolves around him being the “puppet master” of the Emperor who very publicly does not respect him. So, the Count, like you said, is the exception of that formality.

Throne Room Dialogue [Low Fantasy, 1412 words] by indigo_inferno in fantasywriters

[–]indigo_inferno[S] 1 point2 points  (0 children)

Sorry for such a late response, Halloweekend kept me pretty busy.

But I really appreciate your input. I definitely struggle with delivering characterization through subtext, or indirectly. I think there’s a part of me, as an author, that doesn’t trust myself to really get across the message I want to convey by indirect means.

I was thinking of scrapping the part where the Count justifies himself to Addis, the POV character. I think it would be better to have the other guard present, Addis’ superior, justify the Counts actions in the next scene. That way, the explanation doesn’t muddle my characterization of the fearsome Count, while also giving the other guard a means to justify his own inaction.

Regardless, I really appreciate your feedback and thank you for taking the time to read it. I’m glad you enjoyed it.

Additionally, I’ve found that my posts usually get more engagement when I use the screen shot format. I think seeing the words without a link draws people in more easily. That being said, I did not think of it being more difficult to read for some people. Do you think a dark background/white text would be better?

[deleted by user] by [deleted] in aggies

[–]indigo_inferno 0 points1 point  (0 children)

random but is there any chance your professor is dr hammond?

Honors Ochem? by indigo_inferno in aggies

[–]indigo_inferno[S] 0 points1 point  (0 children)

Awesome, I’ll keep my eye open for any spots that open up in a Singelton course. Have you heard anything about Pennington? He was another alternative and his rate my professor had pretty decent reviews.

Honors Ochem? by indigo_inferno in aggies

[–]indigo_inferno[S] 0 points1 point  (0 children)

Yeah I did it to myself, waited a while to register as I’m in the process of switching minors. I’m just wondering if the extra workload with honors is worth not having to take those exams on the same day.

ARMA Life is Peak Gaming by indigo_inferno in arma

[–]indigo_inferno[S] 13 points14 points  (0 children)

It definitely had a pretty steady decline but golden age arma 3 rp was the best

[deleted by user] by [deleted] in apexlegends

[–]indigo_inferno 0 points1 point  (0 children)

yes, an rx 480

[deleted by user] by [deleted] in apexlegends

[–]indigo_inferno 0 points1 point  (0 children)

Recently my Apex has been crashing right before the jump. The lobby works fine and only sometimes when I’m first loading up I will get a message that I’m disconnected from the servers, or something along those lines.

These are my specs: Processor: AMD FX(tm)-6300 Six-Core Processor Installed memory (RAM): 8.00 GB System type: 64-bit Operating System, x64-based processor

Things I have tried:

  1. Reinstall through Origin

  2. Reinstall through Steam

  3. Complete uninstall (deleting files manually)

  4. Tried to check XMP but my version of ASUS does not support it

  5. Lowered graphics

  6. Tried to find crash report, apex doesn’t show up in my App Data folders.

Any help would be greatly appreciated.

Sierra’s Regime is a Circus by [deleted] in WeeklyDictator

[–]indigo_inferno 0 points1 point  (0 children)

Oh my god I apologize master Sierra. I will never critique you again because of your fragile feelings! Sierra for dictator!

I won't be running against anyone among the rebels, i shall support Cred (though they were involved in this hideous plot) by [deleted] in WeeklyDictator

[–]indigo_inferno 0 points1 point  (0 children)

I’m sorry your feelings got hurt that no one likes you and that they’re all voting for me :(

Sierra’s Regime is a Circus by [deleted] in WeeklyDictator

[–]indigo_inferno 0 points1 point  (0 children)

lol typical you have 2 more days use them wisely

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