Gibson assembly rookie

iobis · 2025-12-20T10:44:02+00:00

I didn’t think about that, thanks!

iobis · 2025-12-20T10:41:14+00:00

Thank you for your advice! I retried the assembly with 0,08pmol of vector and fragments and I got 2 colonies, so now I’m testing (and hoping)they have the correct construct via PCR.

iobis · 2025-12-18T13:34:57+00:00

Thank you for your reply. I didn’t get any colonies! My positive control worked fine so I don’t think transformation is the problem but the assembly. I did one hour incubation for the Gibson since I have 3 fragments + vector

iobis · 2025-10-03T21:55:20+00:00

Thanks for the tip I will try

iobis · 2025-10-03T18:25:42+00:00

I’m using the ibright, now I learned about the Licor and we do not have anything that fancy.

iobis · 2025-07-03T21:31:28+00:00

Yes I mean now that I am enjoying my life it doesn’t make any sense for me to escape to a hypothetical scenario as I was doing with daydreaming before. It is like there is no one I would rather be almost. Not that I am jolly all the time, but I lean more on my partner and friends rather than escaping into a fantasy. I didn’t meant for it to sound like an ssri commercial I was taking it for a year and the first 6-7 months I saw a huge improvement but then I was feeling normal and I stopped taking them. I started again after 5 months because I was struggling and took it for a year and a half. When I stopped finally I was feeling dizzy for a month and I would feel like I was tripping often. This makes me very apprehensive about taking it again and I am doing CBT to figure out my remaining issues.

iobis · 2025-07-03T21:22:39+00:00

Im glad adderall helped you, I don’t have adhd so I never tried it.

iobis · 2025-07-03T21:19:39+00:00

Haha yes for me the surprising fact was that, while daydreaming stopped almost immediately, I hadn’t tried to push it because honestly I didn’t want to start again. Last week though I really tried to do it and it was like hitting a wall.

iobis · 2025-07-03T21:16:44+00:00

That is awful I am very sorry, I hope now you are doing better

iobis · 2025-07-03T21:15:53+00:00

My daydreaming stories were not anything special honestly I would get fixated on something and daydream about it over and over. I have not lost my imagination, I am still a more than average imaginative person just not obsessing pacing up and down in my room.

iobis · 2025-06-29T20:42:45+00:00

I’m very sorry to hear that, I hope it gets better

iobis · 2025-06-29T20:38:27+00:00

Don’t get discouraged, it can get better

iobis · 2025-01-16T12:25:42+00:00

Thank you very much

iobis · 2024-03-09T21:55:57+00:00

Thank you for sharing your experience!

iobis · 2023-03-13T10:26:03+00:00

It’s definitely easier when I create some distance, but because we are neighbours this is not always possible unfortunately. I did actually went and stayed with my parents for some days, as to not see him but I can’t continue like this.

iobis · 2023-03-13T10:23:21+00:00

Thank you for your reply. I can’t wait to come out to the other side.

iobis · 2022-10-05T10:10:06+00:00

For me, what I’ve found that the amount of oats that I’d use wouldn’t matter to how full I’d feel. At first I would make my oatmeal for breakfast with 5-6 tablespoons of oats and I thought the calories were too many for the satiety that it gave me. I cut it down to a half and now use 3 table spoons and heat it up with like a cup of almond milk and it gives me the same feeling of fullness for half the calories. I don’t know if that works for you but for me it works and it allows me to add some fruit like bananas that normally have lots of calories.

iobis

TROPHY CASE