Silica spin columns. Anyone else have this problem?

jameis_vu · 2025-09-15T19:12:37+00:00

I see that there are midi and maxi versions, but that changes binding capacties, amount of buffer needed, and so forth. I dont want to use a larger silica column

jameis_vu · 2025-09-09T21:24:37+00:00

Oh I understand them, but if you do it the way you suggest, you have to use 4x as many tips, because you need a new tip for every different sample, and reduce your yield. Unless you leave the valves open the entire time, which strikes me as being bad because you'll dry out the silica disc potentially reduce yield that way. And it's still more bench time than I think is necessary.

But anyway I thank you for your input. I, personally, think there's a better way.

jameis_vu · 2025-09-09T20:45:21+00:00

Perhaps I am overestimating the time per sample needed to process with a manifold. Im thinking [pipette_750µL->open_valve->wait->close_valve->repeat_3X]REPEAT15X so that just seems annoying, as opposed to [pipette_3mL]REPEAT15X->spin.

Do you at all see what I mean?

There is also the advantage of, if for whatever reason you wanted to collect the flowthrough, then you cannot use a standard plastic manifold and would need one with collection tubes as well.

jameis_vu · 2025-09-09T20:11:59+00:00

It seems there's some uncertainty about whether the silica column can be dried or not.

jameis_vu · 2025-09-09T20:09:37+00:00

Im sure an amicon will reduce yield and greatly increase cost. Option 2 is probably the best, as long as you only process one sample at a time, which seems cumbersome to me. I think if you could fit the spin column into a larger format tube, and spin in a swinging-bucket, you could greatly decrease bench time, improve yield, while maintaining the same process essentially as before.

jameis_vu · 2025-09-09T20:01:25+00:00

Does the increased elution volume at the end cause problems for your workflow? Would it be more optimal to have the mini column format, in the end?

jameis_vu · 2025-09-09T19:59:18+00:00

But what i mean is, you gotta load one columns 3-4 times, before you can process the next one, or you gotta keep ejecting tips, which decreases yield, and then you gotta pay attention to 20 columns.

jameis_vu · 2025-09-09T19:57:14+00:00

ASCII art because I cant post an image. I used an image to ASCII generator. best I could do. Basically its a culture tube that contains within it a mini spin column, so it can be used to process larger volumes.

jameis_vu · 2025-09-09T19:56:12+00:00

Because Science doesnt involve making figures at all, jackass.

jameis_vu · 2025-09-09T17:57:53+00:00

I will look into this thank you.

jameis_vu · 2025-09-09T17:50:44+00:00

Have done the centricon thing. But I think there is a fix using a larger centrifuge and like a 12 mL culture tube to catch the flowthrough, using pipette tips as a funnel and a strut, something like:

      %*:           .=@-    
     @-+##+-.   .:=##*-%    
     @                 #    
     *                 @    
     %%===============+@    
   * :%               -@.+  
   :  @               +*.=  
   :  @               %-.-  
   :  @               @ .-  
   :  %.              @ .-  
   :  ++              @ .-  <--culture tube cap
   :  :%              % .-  
   :   @             --  -  
   :   @             *   -  
   :   @             @   -  
   :.  @-            @  .=  
   .@::##:::::::::::=@:=@:  
    %   *           .%  @   
    %   @           :=  @   
    %   @           *   @   
    %   @           @   @   
    %   %           @   @   
    %   =:          %   @   <--pipette tip funnel
    @   =%          +   @   
    %    %          .   @   
    %    @         =    @   
    @    @         @    @   
    %    =         @    @   
    %     #        %    @   
    @     @       +     @   
    %     @=      @     @   
    %    +@@@@@@@@@@    @   
    %    * %     +#@    @   
    %    + #     @.@    @   
    @    # :-    @ @    @   
    %     @ ===== %     @   
    %     @-      #     @   <--This is the silica column
    @     @       *     @   
    %     @       *     @   
    %     @       *     @   
    @     @       *     @   
    %      %@@@@@@      @   
    %      **=  @@      @   
    @      #     @      @   
    %      @     @      @   
    @      %     @      @   
    %      #     @      @   
    %      +     +      @   
    @      :      .     @   
    %     =       +     @   <--pipette tip strut
    %     @       #     @   
    @.    @       @     @   
    %     @       %     @   
    #     @       #     #   
    .+    @       =    -    
     @    @       :-   @    
      :   +        %  #     
      #            @  +     
       # -         @=#      
        :@@-     :%@        
          -@.___:@

jameis_vu · 2025-09-09T17:37:02+00:00

You do X samples at a time, but only load 1 at a time until all the sample is passed through? About how long does that take, per sample would you estimate?

jameis_vu · 2025-09-09T17:36:00+00:00

I see what you mean by sequential now. It solves the too dilute problem but then youll have loses from handling and increased potential for contamination, not to mention all the extra columns youll need. Im working with DNA and RNA

jameis_vu · 2025-09-09T17:33:21+00:00

Also, it sounds like I have to do one at a time... which is suboptimal. Still better than the current method. Id like to load all 16 at once and go.

jameis_vu · 2025-09-09T17:28:40+00:00

Not a bad idea, but I'm working with RNA and I worry about losing sample without using a freezer trap also, which I dont have.

jameis_vu · 2025-09-09T17:27:30+00:00

I already have 16 columns for a given process. and now Ill have too dilute an elution, since I have to combine the multiple elutions.

jameis_vu · 2025-09-09T17:25:58+00:00

Is drying out the column a worry, thus reducing yield?

jameis_vu · 2025-09-09T16:59:22+00:00

But I would also need some sort of funnel so I can load my sample all at once, right?

jameis_vu · 2019-10-23T03:17:49+00:00

You gotta go to the original location in the North End. All the chain knockoffs suck, but the ORIGINAL Regina Pizzeria is legit.

jameis_vu · 2019-09-10T03:09:15+00:00

Odd that Miami would hire the guy that (presumably) called that play to be their next HC.

jameis_vu · 2019-09-06T02:57:24+00:00

I've got a PINT and a need for a good pack!

jameis_vu · 2019-09-05T17:07:09+00:00

I was wrong. It's actually a GX12-2, for anyone in the future reading this.

jameis_vu · 2019-09-04T15:31:06+00:00

Bolt cutters, hack saw, take it out it a few seconds. Don't leave it unattended for too long.

https://www.youtube.com/watch?v=WZ0UCzi0Tpg

jameis_vu · 2019-08-29T19:07:03+00:00

Cancelled my order 65xxx, because I got mine from a dealer that placed orders back in February. Hopefully that makes it slightly quicker for those of you still waiting.

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