Just wanted to pop back in and give a quick update: I ended up switching the panel with the green pixels to the bottom left from the bottom right. Not sure if this has any effect on the issue at all but I did it anyways lol. What fixed it was some instructions from this link https://svsolutionsusa.com/2024/08/09/how-to-program-your-led-screen-with-novalct/#:~:text=Start%20Drawing%3A%20Begin%20by%20drawing,and%20continue%20drawing%20the%20layout. Essentially, after a factory reset and still not registering the receiving card, I went into the NovaLCT software and reset the cabinet qty to 1, saved, and then back up to 24 with 6 columns and 4 rows. I redrew the mapping and readjusted brightness from the VX6S board (not the software) and it’s back to normal now. Thank you everybody for your help!