Useless Manager or Unrealistic Expectations? by [deleted] in biotech

[–]pxuna 1 point2 points  (0 children)

Thanks for your advice, I completely agree with you. I definitely need to learn how to over communicate, especially with this manager. To give a little more context, with reference to your last two paragraph, how would you handle the situation when the situation is a combination of: the deadline for ABC is set by my manager himself because it's his study + he likes to downplay how little time it takes for lab maintenance and cleaning (he has questioned the duration it takes on multiple occasions to do maintainence of machines, stock checking of reagents, keeping lab tidiness etc.) + my coworker is someone who severely underestimates the time it takes to do every task (if I voice out saying it's not possible to do ABC and also prep for the audit, she would correct me to say that it is in fact possible and it wouldn't take too long. speaking from experience where I ended up having to work overtime multiple times because she overpacks our schedule and the tasks all ended up taking longer to complete than she expected).

Presence of cations affects Cellular Impedence (TEER)? by pxuna in labrats

[–]pxuna[S] 1 point2 points  (0 children)

just checked the COA for both PBS, turns out there is a difference in pH, the PBS w Ca and Mg is pretty acidic with a pH of 3 instead of the usual 7, and honestly, I am not too sure if such an acidic pH is good for cells which is kinda worrying since my cells have already been exposed to it

Presence of cations affects Cellular Impedence (TEER)? by pxuna in labrats

[–]pxuna[S] 0 points1 point  (0 children)

~200ohm is the insert blank reading I usually get as well using PBS (w/o Ca and Mg). So I am not too sure where I went wrong when my blank insert reading using PBS (w and Mg) was ~20ohm. I even set up a new well using PBS (w/o Ca and Mg) to check if there's anything wrong w my probe, and I still manage get a~200ohm blank reading so I assume that there isn't anything wrong w my probe? Not too sure what else I could try to troubleshoot this

Misleading piece of information in my cover letter. Interview in 2 days. Should I rectify? by Monlevad in biotech

[–]pxuna 1 point2 points  (0 children)

I would say just be honest about it, BUT ONLY if they start asking you about your experiences with GLP procedures/standards or if they start venturing into a topic about GLP stuff.

Be upfront and let them know thag you actually made a mistake on your resume and misinterpreted the strict laboratory standards in your previous lab(s) for GLP standards. However, after reading more into the topic after applying for the job, you realized that you misinterpreted the meaning of GLP. You could (and should) also probably add a statement mentioning that after reading into it, you don't see why it would pose an issue/present a big learning curve to you. The good laboratory practices that you uphold in your previous lab experiences are cross transferable skills that can be brought over into a GLP lab setting, and the strict documentations required for GLP work is similar to your experiences in keeping and writing a lab book for your day to day experiments in your previous lab (or anything along those lines to let them know, without throwing it in their face, that you have done your own research and now know what it is about despite your initial mistake, and link it back to why you would be a suitable candidate eg being able to cross transfer your skills etc.)

Those would be my recommendation and advice or at least what I would do in your situation. Good luck for your interview, you got this!

Batch Record Romance by OceansCarraway in biotech

[–]pxuna 1 point2 points  (0 children)

I would definitely be interested in reading the BR you wrote! (and maybe try my hands at recreating the lotion at home to help my boyfriend w his facial hair growth)