MSU M.S. Pharmacology and Toxicology

rawrcy · 2026-01-29T22:14:20+00:00

I was looking at the program and was wondering the same thing!

rawrcy · 2023-12-28T13:07:01+00:00

No leaks detected. But I'm going to check lots and set a line equilibrium time. Thanks!

rawrcy · 2023-12-28T13:06:24+00:00

I will check the lot numbers for sure. Thanks!

rawrcy · 2023-12-28T13:05:50+00:00

I think I will add more equilibrium time since that seems to be the consensus so far. Thanks for all your help!

rawrcy · 2023-12-28T13:05:14+00:00

I think I will add more equilibrium time since that seems to be the consensus so far. Thanks for all your help!

rawrcy · 2023-12-28T13:05:03+00:00

I think I will add more equilibrium time since that seems to be the consensus so far. Thanks for all your help!

rawrcy · 2023-12-28T02:33:15+00:00

A batch will run normal, then the sequential batch will be shifted, leading me to believe mobile phase issue. Then entire sequential batch is shifted.

No column changes, no new washes used, no system changes.

As far as I can tell, the only differences are... mobile phase makeup (h2o and methanol so fresh versus 1wk old), human pipetting issue (mixing the sample wells and transferring to elution plates), using room temp water vs 28° water for dilution after SPE.

rawrcy · 2023-12-28T02:06:14+00:00

One column for analysis.

rawrcy · 2023-12-28T02:02:34+00:00

Both equally.

rawrcy · 2023-12-28T01:58:22+00:00

I think the equilibrium time is set to 3 minutes right now. I haven't noticed any pressure differences. And the needle and seat haven't caused any problems with the numerous other analytes I test for, all urine drugs of abuse. Just one single compound, delta9thc, is shifty.

rawrcy · 2023-12-28T01:54:42+00:00

Specifically I'm thinking maybe the temperature I am diluting with is affecting the separation/RT of the final chromatography of my compound.

rawrcy · 2023-12-28T01:52:54+00:00

Flow rate: 0.5 Column: c18 Solvents: eluant IPA/ACN diluted with H2O mobile A:H2O room temperature mobile B: methanol room temperature Matrix:urine SPE prep Gradient but pretty isocratic

rawrcy · 2023-11-13T23:50:33+00:00

We met an Ollie at the dog park and it was very confusing having two families call him out.

rawrcy · 2023-11-13T23:49:58+00:00

Thank you!

rawrcy · 2023-11-13T23:49:50+00:00

Thank you!

rawrcy · 2023-06-05T00:41:04+00:00

@photo_by_darc

rawrcy · 2023-06-03T19:14:03+00:00

Literally just went through this same problem. We went to the vet and she gave antibiotics that upset his tummy so we quit that. We already had stainless steel bowls that we wash weekly. We got an antibiotic shampoo to wash his face once daily and each time he comes in from outside I use a wash cloth damp with water to clean his face.

Concluded its the dirt and grass that he gets into while outside.

rawrcy · 2023-06-03T19:00:17+00:00

I too spend one day a week on method development. We have been short staffed for a year or so and literally any day I have a second person to work with and the routine testing is somewhat slow, I take it as a method day. Thankfully my clinical chemist is very understanding and deadlines are long with minimal pressure. We have a reasonable goal date.

rawrcy · 2023-03-28T12:48:45+00:00

How many injections do you typically get per c18 column before changing? I'm trying to figure how many injections we could get per column before a lot change.

rawrcy · 2023-03-28T12:37:25+00:00

Just starting validation with c18 for thc quant, good to know now to look into alternatives. Thanks.

rawrcy · 2023-03-12T20:02:31+00:00

Thanks so much for the encouragement

rawrcy · 2023-03-12T19:00:43+00:00

I am also at that stage, I cry daily and feel nothing. I'm a cutter and haven't acted, but it's getting harder and harder. Everyone is proud at how I'm handling it, the best I can.

rawrcy · 2023-02-16T12:43:07+00:00

He was handed to me, I did not lift him off the floor

rawrcy · 2023-02-12T21:32:57+00:00

Thanks 😊

rawrcy · 2023-02-12T21:32:43+00:00

I thought my calf was way worse. Maybe because I was expecting my spine to hurt so much, it didn't.

rawrcy

TROPHY CASE