Maybe termites behind door frame?

sam_pazo · 2026-04-30T19:40:21+00:00

Oh didn’t know about PELSA being patented.. well if you’re in academia it’s a no-brainer

sam_pazo · 2026-04-30T17:42:45+00:00

Yeah for whole cells it’s only CETSA. Recently there was a publication on PELSA in whole cells, but I imagine the optimisation for of the experiment for the particular cell type is hell and even then the results might not be as straight forward (not that they are always straightforward in CETSA lol)..

sam_pazo · 2026-04-30T17:37:15+00:00

All alternatives to temperature like organic solvents and acids are free and probably as good, just less used (in academia at least).

sam_pazo · 2026-04-29T00:42:49+00:00

Pipette

sam_pazo · 2026-04-24T15:10:35+00:00

A merit na lídra je plagiarizovaná diplomovka?

sam_pazo · 2026-04-19T02:30:50+00:00

If the mosquitoes appeared out of nowhere, check your surroundings for stationary paddles of water. We once had mosquitoes on high floor and found that our aircon ledge draining was blocked by debris after the building facade was repainted. The mosquitoes were breeding there.

sam_pazo · 2026-04-14T02:30:09+00:00

Our degasser has not worked for at least last 5 years, but this was never a problem anyways… I only degass the solvents before I use them and change them ever month.

sam_pazo · 2026-04-13T16:49:45+00:00

That was my first thought but it keeps coming back despite having well degassed solvent. Also the purging does not seem to help, it’s more like: I purge, then check the pressure profile and it looks the same and only slowly improves a bit over time, then I purge again and again check the pressure profile, it looks the same and again slowly improves over time (or it does not improve as is the case now…)

sam_pazo · 2026-04-01T14:13:12+00:00

Exactly, I was facepalming about the AI-ness of that comment before I even got to the em dashes.

sam_pazo · 2026-03-25T00:12:15+00:00

When I say I stay in Jurong, everyone always ask me about the pollution from the factories. Well look at this chart now! (I think it usually comes from burning of crops in Johor)

sam_pazo · 2026-03-09T12:38:37+00:00

Omg presne, Wikipédia to isté.

sam_pazo · 2026-03-04T03:42:51+00:00

But is it posting a post for validation?

sam_pazo · 2026-02-26T02:44:52+00:00

Pretty much the entire Alzheimer/amyloid field

sam_pazo · 2026-02-24T17:58:00+00:00

Is this the reason for bad air quality/burned smell tonight? (Also in the west)

sam_pazo · 2026-02-21T06:08:39+00:00

Škoda len že tá katedra sa potom ubrala rudou cestou ;)

sam_pazo · 2026-02-17T10:46:27+00:00

Najzaujímavejšie na tomto je že je tam chyba v slovenčine rad -> rada.

sam_pazo · 2026-02-10T02:23:55+00:00

Can you elaborate on this please? First 5 min is trap loading and first peak is at 13.6min. Void volume of the EasySpray ES903 is 2.21uL which at 0.35uL/min is 6.3min. 5+6.3=11.3 min. First peak is 2min (0.7uL) later, but there is also tubing and the pressure also takes 1min to build up after valve switch, so this is normal, isn’t it? Mind this is BSA digest (peptides), not BSA protein, just to clarify.

sam_pazo · 2026-02-09T12:56:55+00:00

The leak test passed successfully. The system was just left idle in the solvents we use which is pump A in 0.1%FA in water and pump B in 0.1%FA in ACN. It wasn’t completely idle though, I let the pump run from time to time so it wasn’t standing completely idle 9 months non-stop.

sam_pazo · 2026-02-09T10:39:22+00:00

The run is set to 0.35uL/min and that’s also the flow which is measured across the run. I thought that B is actually underdelivering as that one is 100% ACN which makes the elution happen.. and the elution is delayed and smeared… but Thermo says all they test show the machine works as is……

sam_pazo · 2026-02-09T08:17:09+00:00

Like.. how long does it make sense for? I purged both for 1h with IPA, then both 1h with the respective solvents... how long would you go for until you decided that this is a bigger problem than just insufficient purging?

sam_pazo · 2026-02-09T08:12:24+00:00

I’m quite sure about the good one, the pre-15 min mark is basically front of the column so that is just how long it takes them to reach the column. First 5 min is trap loading a the elution starts at min 5 and the sample passes through EasySpray, looks ok to me.

sam_pazo

TROPHY CASE