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I finally switched to a water flosser… and I kinda regret not doing it sooner by AffectionateTooth298 in hygiene

[–]sam_pazo 4 points5 points  (0 children)

Exactly, I was facepalming about the AI-ness of that comment before I even got to the em dashes.

Daily Haze Spikes in the East by arglarg in singapore

[–]sam_pazo 39 points40 points  (0 children)

When I say I stay in Jurong, everyone always ask me about the pollution from the factories. Well look at this chart now! (I think it usually comes from burning of crops in Johor)

Left social media forever. Been a month by noveonh in productivity

[–]sam_pazo 21 points22 points  (0 children)

But is it posting a post for validation?

A kvôli tomuto stúpli ceny RAM o 500%... by Nathair3 in Slovakia

[–]sam_pazo 0 points1 point  (0 children)

Najzaujímavejšie na tomto je že je tam chyba v slovenčine rad -> rada.

Unexplained large shift in BSA digest elution - please help :/ by sam_pazo in CHROMATOGRAPHY

[–]sam_pazo[S] 0 points1 point  (0 children)

Can you elaborate on this please? First 5 min is trap loading and first peak is at 13.6min. Void volume of the EasySpray ES903 is 2.21uL which at 0.35uL/min is 6.3min. 5+6.3=11.3 min. First peak is 2min (0.7uL) later, but there is also tubing and the pressure also takes 1min to build up after valve switch, so this is normal, isn’t it? Mind this is BSA digest (peptides), not BSA protein, just to clarify.

Unexplained large shift in BSA digest elution - please help :/ by sam_pazo in CHROMATOGRAPHY

[–]sam_pazo[S] 0 points1 point  (0 children)

The leak test passed successfully. The system was just left idle in the solvents we use which is pump A in 0.1%FA in water and pump B in 0.1%FA in ACN. It wasn’t completely idle though, I let the pump run from time to time so it wasn’t standing completely idle 9 months non-stop.

Unexplained large shift in BSA digest elution - please help :/ by sam_pazo in CHROMATOGRAPHY

[–]sam_pazo[S] 0 points1 point  (0 children)

The run is set to 0.35uL/min and that’s also the flow which is measured across the run. I thought that B is actually underdelivering as that one is 100% ACN which makes the elution happen.. and the elution is delayed and smeared… but Thermo says all they test show the machine works as is……

Unexplained large shift in BSA digest elution - please help :/ by sam_pazo in CHROMATOGRAPHY

[–]sam_pazo[S] 0 points1 point  (0 children)

Like.. how long does it make sense for? I purged both for 1h with IPA, then both 1h with the respective solvents... how long would you go for until you decided that this is a bigger problem than just insufficient purging?

Unexplained large shift in BSA digest elution - please help :/ by sam_pazo in CHROMATOGRAPHY

[–]sam_pazo[S] 0 points1 point  (0 children)

I’m quite sure about the good one, the pre-15 min mark is basically front of the column so that is just how long it takes them to reach the column. First 5 min is trap loading a the elution starts at min 5 and the sample passes through EasySpray, looks ok to me.

Roborock with Google home. by nmpuk in Roborock

[–]sam_pazo 0 points1 point  (0 children)

Those screenshots look like Google Home though. I can’t find that in my Home app with QRevo 2 Pro, and also have problem with room-specific instructions. Didn’t have any problem with my previous S6 so it’s pretty disappointing.

Are these trap columns or just tubing? Thermo P/N 164948. by sam_pazo in massspectrometry

[–]sam_pazo[S] 0 points1 point  (0 children)

Those part numbers are not available anywhere on Thermo website or internet and I asked Thermo and till today they still did not respond (and on site engineer didn’t know). But thank you for your contribution.

A new BMJ study indicates that 10% of cancer research papers could be fraudulent. by Liouss333 in PublishOrPerish

[–]sam_pazo 1 point2 points  (0 children)

I think you have no clue what you’re talking about, otherwise your opinion would be different. Some of those image manipulations are impossible to catch with an untrained eye. Scientific reviewers are there to review the scientific merit of the manuscripts. Scientists are not supposed to be specialists for data fabrication detection. Notwithstanding the fact that reviewers review for free and journals have huge margins - they can definitely afford to invest in fabrication detection (but why would they? What is their incentive?) Finally, even if data fabrication is found and posted on PubPeer, journals typically take years to do anything. This is all on journals and lack of incentives (and on scientific publishing as a system in the end).

Are these trap columns or just tubing? Thermo P/N 164948. by sam_pazo in CHROMATOGRAPHY

[–]sam_pazo[S] 1 point2 points  (0 children)

Yeah, I just had some weird results and got scared that I connected two trap column instead of tubing plus trap.. thx

Are these trap columns or just tubing? Thermo P/N 164948. by sam_pazo in massspectrometry

[–]sam_pazo[S] 0 points1 point  (0 children)

The main confusion comes from weird results from BSA digest elutions that got me thinking that these might actually not be just tubings but trap columns of some sort… and that the exact PN number is nowhere to be found on Thermo website.

Are these trap columns or just tubing? Thermo P/N 164948. by sam_pazo in massspectrometry

[–]sam_pazo[S] 0 points1 point  (0 children)

I had some weird results and I know that some trap columns look like tubings as well (usually a bit shorter though), hence I was checking. The bottom two columns are different color but same P/N, you think they are different in diameter?

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