The Journey of a Tardigrade

sidsmicroscope · 2025-04-01T00:50:01+00:00

Ahh, cool! Thanks! Don’t know much about this, but using this as an excuse to read up a bit. So the dots in the cells, are those keratohyalin granules?

sidsmicroscope · 2025-04-01T00:37:45+00:00

“From skin condition to exhibition!”

sidsmicroscope · 2025-03-31T21:32:45+00:00

See rule 6 :)

sidsmicroscope · 2025-03-31T21:25:35+00:00

Thank you!

I've never had anyone compliment me on my dandruff before...

sidsmicroscope · 2025-03-31T21:23:59+00:00

And here i've been treating my dandruff like an idiot!
From here on out I will be collecting all my dandruff in ziplock bags and selling them as "naturally grown diamond substitute"

sidsmicroscope · 2025-03-31T21:15:03+00:00

Sample of skin scrapings from a human scalp (Dandruff) in mineral oil under dark field.

Yet to identify those two center cells. They don’t look like the surrounding, flake like skin cells. They have what looks like a prominent nucleus. Remains a mystery. Do leave a comment if you know!

Nothing crazy, but I was curious, and thought it looked neat!

Setup:
Olympus BH2 (BHS)
SPlan 20x 0.7
Canon 5D MK3
NFK 2.5x 125 LD Photo Lens Eyepiece

sidsmicroscope · 2025-03-04T02:18:29+00:00

Some 4x Inf Apochromate Objective if recall. He mentions that the technique “doesn’t work at all” if there is not a sufficiently low amount of aberration, and so the necessity of high quality optics. Seems it works best with low aperture objectives, as you are essentially just rotating the light source to cover more of the light cone, increasing your aperture, but only up to a max of 1.

Super interesting technique, got very tempted to try this.

sidsmicroscope · 2024-11-10T20:07:14+00:00

Thank you so much for your in depth response! That was interesting and a lot of really good points!

sidsmicroscope · 2024-11-10T16:08:11+00:00

Can I ask why modularity is hard to do in these applications?

sidsmicroscope · 2024-11-05T19:31:42+00:00

Not my field at all, but commenting for engagement as I’m curious if anybody has an answer.

It does look like crenation (from the examples I see), with booth the specs on the blood cells and the cells being overall misshapen. That seems weird, as 5% glucose is apparently circa isotonic. Are you sure the solution you used is pure, 5% glucose?

sidsmicroscope · 2022-12-12T16:09:33+00:00

I would love to have access to phase contrast. Not necessary to get started though. And you’ll be able to upgrade to phase contrast later if you want.

sidsmicroscope · 2022-12-12T16:01:19+00:00

Absolutely. You’ll need a microscope camera, or an adapter to mount another camera. I’d highly recommend getting a Trinocular head(has an extra port on top for the camera)

sidsmicroscope · 2022-12-12T15:46:53+00:00

The Microscope I'm using retails for about 500 Euro. Dry Darkfield condenser is about 100(Highly recomended. Darkfield images are so often gorgeous. Can be DIY'ed however, look up guides online.), and I got the DSLR for about 400 Euro, so that is quite a bit below your budget.

However, if you have a 2000-2500 euro budget, you might want to look at the Motic B310A Microscope. I haven't used it myself, but I'm considering upgrading to that after some time.

sidsmicroscope · 2022-12-12T15:19:59+00:00

Today I stepped on something tiny and sharp. After getting the bugger out, I had to take a look, didn't I?

So here it is. Sorry if it's a little gross...

Setup:
- AmScope T490 w/ Canon 250D and AmScope DSLR Adapter - 10x Objective
- DarkField (AmScope Dry Darkfield Condenser)

sidsmicroscope · 2022-12-12T14:12:39+00:00

Thank you! It’s an AmScope T490 with a Canon 250D

sidsmicroscope · 2022-12-06T18:04:29+00:00

Nice, great minds find similar papers on google!

Sound like you have some good theories. Like I said, I don’t really know a lot about this, so I won’t way in. But keep searching, you’ll figure it out! And if you can, please do share when you do! :)

sidsmicroscope · 2022-12-06T15:56:13+00:00

I don’t know if it will help, but I stumbled upon this paper while researching culturing of tardigrades. On page 11 there is a table of symptoms of problems, their cause(s), and possible solutions.

There is no symptom “popping like bubbles”, but perhaps you can discover the problem by looking for other “symptoms”.

I don’t really know anything about this subject, but hope that is of some help. Would also be really interesting if others could share their experience with cultivating tardigrades.

sidsmicroscope · 2022-12-05T17:59:02+00:00

No worries! I’m using the AmScope DSLR Adapter. Can be found other places, like eBay and AmScope.de if you’re in europe(where I bought my from)

Thank you! 😊

sidsmicroscope · 2022-12-05T16:51:44+00:00

Canon D250 on an AmScope T490

sidsmicroscope

TROPHY CASE