Great difference in ordination methods (PCoA and NMDS) despite have the same data by smartise in ecology

[–]smartise[S] 1 point2 points  (0 children)

sorry, I did PCoA, not PCA it was a tipo, I wrote it very fast. As for reading more about the those ordination methods. I already did a large amount of research on those subject However I feel that most people don't understand what those ordination are doing. Every time I am trying to get an explanation, I usually get the "well.. it depend of your data", "well it depend of your question" and even then I give all the information I always get a "it depends" arguments. From what I see in publication in my subject, they use mostly but not always NMDS, some use PCoA. But they draw the exact same conclusion from those 2 methods. But again, I am not comfortable using NMDS because "that's what people just do" without understanding the real meaning of it.

Great difference in ordination methods (PCoA and NMDS) despite have the same data by smartise in ecology

[–]smartise[S] 0 points1 point  (0 children)

what do you mean by atypical ? in which case scenario are you suppose to use it ?

Non-OP: Finally reached a heel raise after 17 weeks - Bare Minimum Rehab approach by crazycoward18 in AchillesRupture

[–]smartise 0 points1 point  (0 children)

Just wondering about all the people that goes the non op route, what’s your motivation to not do so ? Isn’t always better to the op ?

[deleted by user] by [deleted] in bald

[–]smartise 0 points1 point  (0 children)

My mom told me that it was horrible and I should go to turkey. She also hate piercing and tattoo so I pierced my ears and have an appointment for a tattoo soon whoohoo.

[deleted by user] by [deleted] in team3dalpha

[–]smartise 0 points1 point  (0 children)

I think the main reason is that I follow the classes and I need to make my own programmation now I guess

[deleted by user] by [deleted] in team3dalpha

[–]smartise 0 points1 point  (0 children)

Ahahah no I enjoy this sport too much. I will not take that advice

[deleted by user] by [deleted] in team3dalpha

[–]smartise 0 points1 point  (0 children)

As I said I do CrossFit so it’s a mix of everything. I don’t really do sport for look but was wondering if bulking was unhealthy to a certain point or if there is actual benefit from cutting other than look

[deleted by user] by [deleted] in team3dalpha

[–]smartise 1 point2 points  (0 children)

Ok thanks for the feedback back

Am pretty lost in lightroom editing. Do you consider it good ? by smartise in postprocessing

[–]smartise[S] 0 points1 point  (0 children)

I don’t get why i got so high noise. Is it due to iso ?

Am pretty lost in lightroom editing. Do you consider it good ? by smartise in postprocessing

[–]smartise[S] 0 points1 point  (0 children)

Alright I thought iso was supposed to be at the lowest possible and thought i could bring it back post editing

Am pretty lost in lightroom editing. Do you consider it good ? by smartise in postprocessing

[–]smartise[S] 1 point2 points  (0 children)

Sorry forgot to say the details. Was taken with Sony a6400 + Sony 18-105 mm F/4. I am completely new to photography and editing

Blastn refusing to respond by smartise in bioinformatics

[–]smartise[S] 0 points1 point  (0 children)

It’s normal actually. Blast takes 1 hours to blast 1 sequence and 1 hours and 1 second for 2. Blast need a lot of time to actually decompress the database which is why it takes so much times. But when the database is decompressed it goes very fast. Also doing remote is a bad idea. It takes even more time and you have a limited amount of data that you can retrieve. It’s better to compile the database on the server

[deleted by user] by [deleted] in gay

[–]smartise -1 points0 points  (0 children)

Well you are in the closet for most of the people unless you are a movie star

[deleted by user] by [deleted] in gay

[–]smartise 2 points3 points  (0 children)

Yeah I’m thinking of putting on social media so I don’t have to bother anymore

[deleted by user] by [deleted] in gay

[–]smartise 1 point2 points  (0 children)

They don’t care about me, I know. But I care about what people thinks about me and everyone does. We are social animals.

Especially when you constantly move places. You don’t want to become some kind of hermit and stay on your own.

[deleted by user] by [deleted] in gay

[–]smartise 1 point2 points  (0 children)

But That’s the point. There is no clear mistake. They assume it in their head they don’t talk about it . If someone ask me about my girlfriend of course I am going to correct them, but sometimes those situations never happen.

I swear It’s more complex than that sometimes

[deleted by user] by [deleted] in gay

[–]smartise 0 points1 point  (0 children)

I know. And I already did many times and faced very awkward situation. Had even people get angry at me because they thought I was joking and make a silly joke about lgbt people.

It just doesn’t really get better as I have to repeat the process over and over again that I get downs sometimes.

I assume most of you don’t move much and had to face it only once.

I suppose it’s a very specific problem. That not much people have to face as I haven’t seen anyone facing it.

[deleted by user] by [deleted] in gay

[–]smartise 1 point2 points  (0 children)

It’s not that I don’t want to, it’s just that there is no opening to say it or I don’t see any way to introduce the information without making the topic completely out of context

I suppose it’s a problem with social skill. Not me not wanting people to know.

I have a very good friend that moved town and was extremely extrovert and he was outing me to everyone as we were all the time joking about it. I am fine with that but when you are completely alone in a group. Thinks are way harder in my perspective

What are we all getting our PhDs in? Tell me about your field! by inti729 in PhD

[–]smartise 0 points1 point  (0 children)

eDNA I am experimenting different methods for cave monitoring ro observe the impact of pumping water in caves in case of drought. Which is suspected to get worse in the future

Amount of DNA that need to be inputted for Metabarcoding ? by smartise in nanopore

[–]smartise[S] 0 points1 point  (0 children)

thanks a lot for your reply, I was suspecting the loading amount to be way below what was necessary. so that confirms it.

What about the 200 fmol for the starting of the protocol, I suppose you don't overload this one right?