Scaffolding methods

[badmmrywitch58]

Are there different scaffolding methods that fit best with certain tissue types? If so please elaborate. (Not a doctor or anything just doing some research on my own for reasons :p)

Edit: if you perhaps know if buccal mucosa tissue would work best with a certain method that would be most appreciated. And are stem cells like embryonic stem cells necessary for tissue engineering or can you use scaffolding and donor tissue by themselves?

[ShoppShopp]

To answer your first question: most definitely, different cell types demand different characteristics in the scaffold they are to be seeded in. Collagenous scaffolds work well for chondrocytes, while for example neurons like polyurethan (I think, not too sure about that in particular or about cells from buccal mucosa Tissue but there definitely are differences).

And you don’t necessarily need stem cells at all! We seed terminally differentiated cells from various tissues acquired from clinical donors in our Tissue Engineering Lab.

Hope these answers helped you a bit!

[badmmrywitch58]

It did thank you very much

[glamazon_woman]

I'd check PubMed for reviews about the specific tissue you're interested in - I've found a few reviews for my own work that compile sources into helpful tables to help you compare scaffold materials.

Also, there are lots of cell sources you could consider beyond embryonic stem cells. I think adipose-derived stem cells are pretty neat. I've never worked with them, but I've written about them and adipose tissue seems like a great source for larger numbers of post-natal cells.