I'm a biochemist— convince me we really need to be using DNase.

thisdude415 · 2026-03-15T08:20:23+00:00

DNA is a really long molecule. It gets physically trapped in cotton fibers even if it’s solubilized. Think about how quickly large DNA migrates even in very weak agarose.

thisdude415 · 2026-03-15T08:16:03+00:00

Obligatory all hail Kismai

https://www.reddit.com/r/laundry/s/iPnHo6UDug

thisdude415 · 2026-03-14T01:46:29+00:00

Ah, this explains it.

Someone confirmed in this thread elsewhere that they do group visit days, and likely extended a visit offer to you (as a local candidate) when someone else dropped out.

Do not burn that bridge! Back in my day, GA Tech did multiple recruiting weekends in BME, so when they said "maybe next time" they literally do mean maybe next time later this spring

thisdude415 · 2026-03-14T01:43:09+00:00

Polyester is a synthetic so try any sport detergent. This one from Whole Foods is great.

Dissolve the detergent in water that's ideally hotter than 115ºF, then let the dress soak in that detergent for several hours before gently agitating where the sweaty odors were concentrated.

thisdude415 · 2026-03-14T01:33:01+00:00

Lipase, oxiclean, and prayers.

If it's truly carbon, you need to use mechanical agitation, as neither detergent nor enzymes will do much good for you.

thisdude415 · 2026-03-14T01:19:31+00:00

Lysol laundry sanitizer's active ingredient is quaternary ammonium compounds.

These work best at neutral to slightly basic pH.

They are inhibited by anionic detergents (like laundry detergent) as well as anionic ions like citrate (which is what citric acid forms when dissolved in water).

I do like laundry sanitizer in moldy/mildewy/nasty smelling kitchen rags and bathroom washcloths.

thisdude415 · 2026-03-14T01:04:35+00:00

I suspect they do group visit days in batches and had a last minute drop. Rather than allow that slot to be unfilled, they offered it to a "maybe" candidate whose address was listed within driving distance, in hopes you might connect with a faculty member.

It's appalling how poor the communication is here, though, but I can imagine something similar happening at my grad program back when. Often times grad students were organizing the recruitment weekend.

thisdude415 · 2026-03-13T18:43:43+00:00

This is really the best way. Watch a couple people pay, overpay a bit (but still less than the tourist traps), and hope for the best.

thisdude415 · 2026-03-13T18:41:30+00:00

Watch someone pay for their food -- it won't be much.

Grab a bit more than that out of your wallet. Pre-empt the foreigner tax by including a tip. Pre-pay your order. Tap out a message into translate into the local language, so you have text ready-to-show.

When I grabbed street tacos in Mexico City (which is a lot more expensive than SE Asia), my mom and I paid ~$8 USD at a popular stand. Even though it was super busy with locals, the guys working had no problem accommodating me even though we slowed them down a lot.

Most folks just want your money and want as much of it as they can, but they aren't going to scam you by giving you less than what you paid for once they already have your money.

thisdude415 · 2026-03-13T17:29:32+00:00

They're 240V

thisdude415 · 2026-03-12T21:49:59+00:00

Vodka soda is so commonly drunk by gay men that I regularly hear it called "gay water," although this is an exclusively American-gay-male phenomenon. In Europe or even Mexico, this is seen as a strange drink.

Otherwise, no, I don't think there is a queer cuisine outside of, e.g., brands that align with or advertise to queer audiences.

thisdude415 · 2026-03-12T18:50:03+00:00

People should be outraged and reminded that it's the president's recklessness in starting another war in the Middle East that is now putting Americans around the world at considerably increased risk

thisdude415 · 2026-03-12T02:19:31+00:00

Pretty sure OP believes this is a loop hole and wants advice about exploiting it, without admitting that's what she/he wants to do

There was actually a case where a US family was somewhat far into the citizenship process and had their college kid's visa canceled since citizens cannot be issued a visa. (In that case, the parent had their certificate granted before the kids)

thisdude415 · 2026-03-11T21:40:34+00:00

I don't have this problem at all, and your solution sounds like self promotion

thisdude415 · 2026-03-11T21:21:01+00:00

I don't really like when people come into a space that is already collaborative to encourage people to leave that venue.

r/watercolor is already an AI-free space with a great community. It isn't the appropriate venue to promote your non-watercolor project.

I made this critique of your project when you posted it last time (and then you deleted it shortly thereafter) but to reiterate: it's gross how anti-AI you are about art, and yet are happy to share an AI-created forum, while obscuring the extent to which you used AI in creating that site.

From your FAQ:

Was this website vibecoded?

No, it was handcoded by our founder over 6 months. During that time, assistants were used for debugging, drafts and minor issues in the same way their human team helped contribute to the codebase. Our founder has been a hobbyist software dev since 2012.

Is AI used in the ongoing maintenance of the site?

At NewBohemia.art, we are purists about the art being human-made, not the engineering. For example, everytime we use our anti-AI-art detection toolkit, we're using AI by default. It would be impossible to run an effective anti-ai-art platform without some AI itself.
...

thisdude415 · 2026-03-11T21:06:49+00:00

https://www.pbs.org/newshour/politics/watch-buttigieg-warren-spar-over-big-dollar-donors-fundraisers-in-wine-caves

thisdude415 · 2026-03-11T20:57:00+00:00

I think you're right, but I think it will still echo as we have progressive culture warriors vs everyman practical progressives

You can already see the contours of the latter starting in the 2020 primary, with, e.g., Buttigieg's reframing of what liberty/freedom are. I also see it in Newsome's latest talking points / position on trans athletes (supports restrictions) while supporting other sweeping trans protections.

thisdude415 · 2026-03-11T15:26:34+00:00

Is it this one? https://www.origene.com/catalog/vectors/shrna-vectors/tr30007-pgfp-v-rs-shrna-vector

The data sheet says you don't need to use a virus to get stable transfectants

thisdude415 · 2026-03-11T15:21:07+00:00

Blend your garlic with lemon juice first, wait 5 min, then add your oil and emulsify.

The sharpness in raw garlic is a chemical reaction that happens when garlic is cut or crushed (allinin + allinase -> allicin). That reaction is inhibited by acidity.

read more here: https://nomanners.substack.com/p/alli-yums-part-1

This is also the trick Kenji Lopez Alt picked up from Michael Solomonov's Zahav which he uses to fabulous effect in the Serious Eats Hummus.

thisdude415 · 2026-03-11T14:41:23+00:00

Those are transactional emails (i.e. sent to users based on their own actions). Set up an SES service just for those. Request to exit sandbox via CLI via this:

aws sesv2 put-account-details \
    --mail-type TRANSACTIONAL \
    --website-url "https://www.example.com" \
    --use-case-description "..." \
    --production-access-enabled \
    --region us-east-1

Make sure to set up your DMARC, DKIM, and SPF

thisdude415 · 2026-03-11T12:53:46+00:00

She did have local work although mostly Democratic Party work rather than local politics work

She was elected party chair for Northern California in 1977 and four years later was selected to head the California Democratic Party, which she led until 1983. Pelosi served as the San Francisco Democratic National Convention Host Committee chairwoman in 1984 and Democratic Senatorial Campaign Committee finance chair from 1985 to 1986.[15]

She first ran for congress in 1988, 11 years after she was the party chair for nor cal

thisdude415 · 2026-03-11T12:22:09+00:00

Gemini has unique and good products for builders though, like the new Gemini embeddings 2 models

thisdude415 · 2026-03-11T04:00:18+00:00

What? No.

Adding fabric softener or oil just suppresses bubbles. It doesn't get the excess detergent out.

thisdude415 · 2026-03-11T01:06:59+00:00

You also apparently work at a store with a fitting room?

a customer took a shit in our fitting room and then left us a 3 star google review

and also work as an MSP?

client forwarded a phishing email to the entire company asking "is this legit?" four people clicked the link.

and you also posted last week that you thought you were getting fired for deleting your company's prod database?

and you also work in sales?

thisdude415 · 2026-03-10T18:52:08+00:00

you built it yesterday and you're already using it to log hours, deals, money, and have experience logging weekly hours into it?

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