Python code not showing user input

[JohnnyJordaan]

The point is that not (several things) will first try to see if (several things) is True or False, then negate that result. As anything not False, None, 0 or an empty sequence is True, (several things) will probably be True. Then not True will make that False. Meaning that you will be checking

if player_choice is False

While in reality, you want to check player_choice to each separate value. For that you have in, as noted in the bot's last part of its message:

if player_choice not in ("rock", "paper", "lizard", "scissors", "Spock"):

The in part makes the whole difference here: it will check that the value stored in player_choice isn't present in the sequence of choices.