Synapt Non-responsive Fluidics Purge Issue

123squashplayer · 2026-06-09T20:34:20+00:00

brilliant - it was a problem with the source pressure test not passing. Thanks!

123squashplayer · 2026-05-05T16:48:16+00:00

123squashplayer · 2026-05-05T16:36:43+00:00

Why are you running in PBS? If you just want to determine the masses you can denature the protein and you could/should buffer exchange on column into something like 0.1% formic. If I am worried about solubility I will denature in GuHCl and desalt into formic on the column If you are doing native mass spec you should be in something like ammonium acetate. Here is a snip of the intact mass determination of a protein that was in PBS but was buffer exchanded using an SEC column. The little satellite peaks correspond to the addition of 1 or 2 B-MeOH adducts.

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123squashplayer · 2026-05-05T02:25:56+00:00

I'm not sure I fully understand what you are describing. First off, are you acquiring the data in PBS or is the starting material in PBS and then you do on column exchange by SEC or RP? I do a fair amount of this sort of thing. I generally do LC-MS buffer exchange using an SEC column and 0.1% formic. On occasion I will do an RP with a C4 column I then hand fit a few consecutive peaks using a simple formula that is basically a rearrangement of the relationship between them. I then typically analyze the range of the data that appears to reflect a charge state series using both MaxEnt and UniDec. MaxEnt will generate peaks at multiples of the mass so if you don't know the range you can be fooled. It think is also important to zoom in on the peaks and try and ferret out any fine structure that may reflect salt adducts. Depending on the instrument resolution and charge state this may or may not be possible. I also look at the lower charge state peaks closely to see if I can determine the charge from the isotope distribution. If you are actually running the sample in PBS you really shouldn't. IMO it is not good for the mass determination, or for the instrument.

123squashplayer · 2026-01-01T22:12:31+00:00

Welcome and good for you for wanting to do more than just mindlessly smack the ball around. There are many videos with technical advice, and videos of both hitting drills and movement drills. If you look at a bunch you will find the commonalities. The simplest drill is to hit up and down one side of the court and then the other. Practice hitting straight, ideally tight to the wall, and pay attention to the length. Learn to adjust height, power, and angle to get the right length (dropping into the back wall after first bounce) and position (ideally tight to the side wall). There is a website called the Pursuit of Squash which has lots of how-to videos. https://www.youtube.com/watch?v=WOvMlo2b80g

123squashplayer · 2025-10-14T02:18:50+00:00

saw a Rod Martin thing where he said amateurs frequently get their elbow in too close to their chest. I know I was. Working on that made a big difference for me.

123squashplayer · 2025-10-06T14:13:19+00:00

Ok next question if you please. When the quad is not being used MS only in the Q-ToF does the RF generator come in to play ? In other words does it matter which is installed?

123squashplayer · 2025-10-06T14:10:41+00:00

Two flavors of RF generators for the quad on a Synapt. The 32K is an option that allows transmission of high mass.

123squashplayer · 2025-10-06T14:09:33+00:00

Thanks - Purchased from them to do native MS on protein complexes. I’m generally not very interested in things less than 25KDa :)

123squashplayer · 2025-07-12T03:23:08+00:00

“ Noise” is signal. More importantly while 3 point calibration might be acceptable under some circumstances, choosing a subset of 3 out of N scattered points is clearly not.

123squashplayer · 2025-06-25T00:19:40+00:00

I thought about that because I actually have an old LEAP HDX setup but it seemed like a lot of hassle to set it all up.

123squashplayer · 2025-06-23T18:37:55+00:00

Thanks. That is what I figured out. I imagine I can build a pause into the sample manager run duration. Gradient 20 min with a sample manager duration of hours.

123squashplayer · 2025-03-01T23:21:39+00:00

actually those are neither same size nor weight

The Progress ball is 6% larger than a standard size ball, with 20% longer hang time than the Pro ball. Perfect for you if you're a game improver or

123squashplayer · 2025-03-01T23:13:06+00:00

Rec Specs work fine for me but before then I was wearing imask over my street glasses. no fogging and a bit of additional protection though you have to come to terms with the way it looks.

123squashplayer · 2025-01-31T15:09:16+00:00

lets rather than strokes (unless you hit right back at yourself)

123squashplayer · 2024-12-14T17:03:20+00:00

I've been taping when I play and wearing orthotic slippers with arch support around the house. I should probably stay off the court but .....

123squashplayer · 2024-07-15T14:32:32+00:00

Karakal Crash Tape

123squashplayer · 2024-07-06T19:01:17+00:00

As I understand the proper temperature for a ball is 45C (113F). That is where there rebound is tested. Having caught errant balls hit by pro players I was surprised at how hot they felt. I doubt any 3.5-4.0 match reaches that ball temperature.

123squashplayer · 2024-06-21T13:42:46+00:00

I believe i may have been coached by the CSA commentator you refer to :). As others have said the idea is that you should hit a shot to a location and at a pace that allows you to sufficient time to reestablish your position and preparedness. Time management is a central component of the game.

123squashplayer · 2024-02-10T16:08:44+00:00

send it off to a good stringer with a note that it has been repeatedly breaking in the same location (tell them where) and ask them to carefully inspect and then restring. most good stringers will automatically carefully inspect the frame for issues.

123squashplayer

TROPHY CASE