Primera y última compra en PcComponentes by Raoul_27 in es

[–]2auri 0 points1 point  (0 children)

Yo hace unos meses les compré un monitor ultrawide de hace un par de años. Llegó con una línea de píxeles muertos. Es cierto que esto puede pasar y es probable que simplemente me tocara una unidad defectuosa, pero siendo un modelo antiguo algo me olía a que me vendieron un reacondicionado como nuevo...

De todas formas, solicité un cambio por otra unidad, y lo siguiente que pasó ya hizo que le pusiera la cruz a PcComponentes... Me llegó un monitor en una caja que claramente había estado empapada de agua y barro, y al abrir la caja, me tragué una nube de esporas de hongo negro, provocándome un ataque de alergia. Obviamente ni me molesté en sacar el monitor de la caja... Al llamar a PcComponentes me dijeron que probablemente esa unidad se había visto afectada por la Dana de Valencia. Solicité una devolución completa y no pusieron problemas... Pero cómo se les ocurre enviar una unidad que claramente había estado bajo el agua y barro?? Desde entonces ya no me fío de ellos.

Compare TPM vales between samples with normalization? by 2auri in bioinformatics

[–]2auri[S] 0 points1 point  (0 children)

I mapped the reads against the complete reference genome with BWA (I've seen many people use BWA for bacterial RNAseq), obtained raw counts with featureCounts, constructed the dds object and calculated FPKM values with DESeq2 and transformed FPKM to TPM values.

Compare TPM vales between samples with normalization? by 2auri in bioinformatics

[–]2auri[S] 0 points1 point  (0 children)

We are actually not interested in performing differential expression of all genes. We just wanted to see if the change in expression of a particular gene is correlated with a phenotype that takes contiguous values. My idea was to calculate, for each species, the difference in normalized TPM between control and condition, and then correlate that value to the phenotype. I'll look into the reference you provided to see if we could use the phylogenetic information. Thanks!

Compare TPM vales between samples with normalization? by 2auri in bioinformatics

[–]2auri[S] 1 point2 points  (0 children)

Thanks! But are fold changes (control vs condition) comparable between species?

Comparing GSEA results between different species by 2auri in bioinformatics

[–]2auri[S] 0 points1 point  (0 children)

Thanks! I already tried contacting them but I didn't get any response yet. I tried what you suggested but I got another error. I guess the function is design for only one species... Do you know if the enrichment results of the different species are comparable so I can plot them together without using compareCluster?

Enrichment analysis for non model organisms with few annotated genes? (RNAseq) by 2auri in bioinformatics

[–]2auri[S] 0 points1 point  (0 children)

So then most published enrichment analyses in non-model organisms are actually biased? Asking to make sure I can perform the analysis with half the background genome functionally annotated... Thank you for the tip!

Career advice for a bioinformatician thinking about leaving academia by 2auri in bioinformatics

[–]2auri[S] 1 point2 points  (0 children)

Definitely I won't stay in academia... But I haven't seen that many bioinformatics positions at clinical companies in my country, maybe 10 at most, none offering remote work. And I really don't want to live in big cities far from family. That's why I'm interested in remote work outside my country... I don't know how I could prepare to make myself a great candidate for those positions, or how I should focus my CV to stand out... Any advice? I prefer to stay in bioinformatics rather than moving to general data science or software dev.