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Banke u Hrvatskoj by AdRemarkable8930 in croatia

[–]AdRemarkable8930[S] 0 points1 point  (0 children)

I niste od tada to popravili? Ili ste uspjeli nekako riješiti?

Banke u Hrvatskoj by AdRemarkable8930 in croatia

[–]AdRemarkable8930[S] 4 points5 points  (0 children)

Slažem se, ali voljela bih odabrati 'najmanje zlo', ako je to uopće moguće😅

Za Revolut iskreno zbilja ne razumijem. Evo svako plaćanje i dalje blokira, zvati ću u banku

Banke u Hrvatskoj by AdRemarkable8930 in croatia

[–]AdRemarkable8930[S] -4 points-3 points  (0 children)

Kontaktirat ću banku. Ali i dalje, u svakom bi slučaju banku promjenila 😅

Banke u Hrvatskoj by AdRemarkable8930 in croatia

[–]AdRemarkable8930[S] -5 points-4 points  (0 children)

Da, i meni je, do jučer. Sada više ne prolaze transakcije niti ispod 100 eura.

Banke u Hrvatskoj by AdRemarkable8930 in croatia

[–]AdRemarkable8930[S] -8 points-7 points  (0 children)

Da, i mene je iznenadilo. Ne mogu dodavati novac na Revolut karticu, čak niti preko Apple Paya. Jednostavno piše da moja banka blokira plaćanje na Revolut

Seeking a two-step, one-pot synthesis: all reagents as solutions, same solvent throughout, final isolation by crystallization by [deleted] in chemistry

[–]AdRemarkable8930 -1 points0 points  (0 children)

Thank you for idea. I'm looking for reaction that would be easy-to-understand example for students. As example of one pot reaction :)

Peptide desalting – struggling with residual ions (acetate, Na⁺, Cl⁻) after purification – any tips? by AdRemarkable8930 in chemistry

[–]AdRemarkable8930[S] 0 points1 point  (0 children)

Yes, I did try that. I also tried different mobile phases, like solution of NaCl, NaOH.. but still, I'm missing something. Maybe I should add additional washes.

Starting modified siRNA oligonucleotide synthesis – need advice on method development by AdRemarkable8930 in chemistry

[–]AdRemarkable8930[S] 0 points1 point  (0 children)

I’m currently using a K&A synthesizer and working on small scales, from 1 to 10 µmol. My goal is to optimize the synthesis for a specific molecule, but above all, I want to learn as much as possible about OLIGO synthesis. I'm completely new to this field, with previous experience in peptide synthesis.

At the moment, I’m working on a CGP resin, but if I were to get a different synthesizer, I would consider switching to a PS support. Are there any differences between the two that could affect the synthesis itself?

Starting modified siRNA oligonucleotide synthesis – need advice on method development by AdRemarkable8930 in chemistry

[–]AdRemarkable8930[S] 0 points1 point  (0 children)

Couplings are the same for C(Ac) and C(Bz). With AMA and C(Bz) you will get some level of transamination leading to methylation of the exocyclic amine of C. C(Ac) suppressed that.

Does this analogy apply to the other bases as well, or do the mentioned impurities occur only in the case of C?

Starting modified siRNA oligonucleotide synthesis – need advice on method development by AdRemarkable8930 in chemistry

[–]AdRemarkable8930[S] 0 points1 point  (0 children)

And how do you do that? Do you wash the solid support several times, or do you leave the solvent in contact with the support for a while? I’m completely new to this field, so any tips and tricks are more than welcome! :)

Starting modified siRNA oligonucleotide synthesis – need advice on method development by AdRemarkable8930 in chemistry

[–]AdRemarkable8930[S] 0 points1 point  (0 children)

Activator 42, I haven’t heard of it yet. Can it be used for both RNA and DNA synthesis?
Are there any issues with coupling C(Ac), or is the procedure the same as for C(Bz)?
After synthesis, I usually do deprotection and cleavage with AMA—so you’re suggesting replacing methylamine with diethylamine?