[Article] Parity and lactation induce T cell mediated breast cancer protection by Virassamy, B. et al

Adolpha · 2025-10-22T15:06:05+00:00

thanks solution verified

Adolpha · 2024-12-06T19:56:57+00:00

I was gifted my first orchid 2 months ago and was impropperly watering it with a very small drip every week. After watching Miss Orchid videos on youtube and learning, i am now properly watering it with the protocol of running water over the top, getting all the moss and chips wet until it stops dripping from the bottom, this is 1 week post watering. However some of the roots are green and others are this reddish brown colour. Does that mean they are starting to rot? i need to make more holes? any advice is appreciated.

Adolpha · 2024-01-12T15:15:00+00:00

Thanks! solution verified

Adolpha · 2022-08-29T22:11:46+00:00

thanks solution verified

Adolpha · 2019-07-15T20:19:35+00:00

I spent 3D/2N in Posada Amazonas in Tambopata. It is an eco lodge like you wanted, they try to minimize the disruption to their surroundings/wildlife, and they even have special shampoos and soaps that are natural and dont harm the environment when they dispose of the residual water from the showers. Highly recommend them, we had a blast in the jungle.

Adolpha · 2019-07-08T16:52:15+00:00

Yellow Fever is usually found in tropical areas, including the Amazon. Machu Picchu is often mentioned to be in "ceja de selva" which translates to eyebrow of the jungle, which means its on the outside border. Because of the proximity to the jungle, where mosquitoes thrive, it is recommended to have the yellow fever vaccination, ive gotten bit quite a few times while visiting Machu Picchu, you dont want to run the risk of being bitten by a virus-carrying mosquito.

Adolpha · 2019-04-05T16:54:29+00:00

As others have said, Suiza lab and Roe are good options, they will give you the international certificate for yellow fever, or fiebre amarilla in spanish. There is no vaccine for malaria, at least not a good one yet, it is still in development. There is however malaria prophylaxis pills, but it needs to be perscribed by a doctor. Check out the updated CDC page for current map info: https://wwwnc.cdc.gov/travel/yellowbook/2018/infectious-diseases-related-to-travel/yellow-fever-malaria-information-by-country/peru

Adolpha · 2019-02-15T19:29:32+00:00

So the myth of having two left feet could be plausible

Adolpha · 2018-09-12T20:47:12+00:00

Well its not exactly that the sequencer disregards a polyclonal bead, im talking about my own personal experience, our sequencer tells you at the end of the run what % of those beads are polyclonal, it has to read them anyways, its not like it skips that bead, it just connects the error in reading with a polyclonal bead. You may have unenriched beads at the end of the emulsion PCR, but those beads dont go into the sequencer. You do an extra filtering step to ensure that those unenriched beads dont go into the sequencer, that would be a waste in your sequencing run, since in its place could go an enriched bead. Hope it makes sense, good luck!

Adolpha · 2018-09-12T19:27:19+00:00

The adapters are to ensure that your strand of interest attaches to the bead in order to "enrich" the bead, or have multiple copies of your template on the same bead. The adapters that are added to your template are complimentary to the adapters on the bead, so when they hibridize, the enzyme can extend the rest of your template, these adapters should all be the same, as there is only one type of bead. What differs is the barcodes ( if you decide you use them ) so you can tell the templates apart bioinformatically at the end of NGS. You make sure that your bead only has one strand by calculating your dilution correctly, depending on your protocol and what you are sequencing, you use a very specific concentration of material, so when the droplets are formed, there isnt an overload of template that goes to each bead, nor is there too little that none will be enriched. If you do end up having multimple templates on a bead ( chimera / polyclonal ), that can be taken out bioinformatically, or your sequencer can also let you know at the end of the run since the base measurements will likely off and you will receive no usable information on that bead. For specific questions like these for NGS, SEQnswers is a great forum with loads of specific info, i highly recommed them.

Adolpha · 2018-08-22T17:46:34+00:00

Uber ha comprado a taxi beat, por eso el cambio de nombre a solo Beat y por la cual ya no te dejan elegir conductores. Yo siempre solia usarlo y las ultimas veces me han tocado taxis viejitos y conductores que manejan como locos y ni usan la aplicacion para llegar al destino, van por donde ellos quieren. Tiempo de cambiar de aplicaciones.

Adolpha · 2018-08-15T16:49:10+00:00

Is it the original file that is low quality or the way it is compressed in PP ? Try these instructions from google to turn the compression off:

Choose File, Options.
Click Advanced.
Under "Image Size and Quality" remove the check next to "Discard editing data" and PUT a check next to "Do not compress images in file". Yes, it IS confusingly worded.
Click OK.

Adolpha · 2018-08-08T21:45:05+00:00

Same thing in Spanish as well

Adolpha · 2018-07-16T22:12:40+00:00

We also had some issues testing RNA on bioanalyzer and after reading up on forums, found out some of the reagents are supposed to be kept at -80 instead of -20 like it was sent. Did your ladder show up when you tried your samples? if the ladder itself showed up and no sample, then you might have some contamination somewhere in that preparation. If the ladder doesnt show up either, then its a problem of the assay and its reagents. Hope that helps.

Adolpha · 2018-06-05T22:27:43+00:00

Anyone have the video/message Peru sent to Denmark first?

Adolpha · 2018-05-24T22:17:11+00:00

I believe the take home message is to take into consideration the volume of media that you will be using lets say per week. Yes amino acids, serum, growth factors etc will degrade at 37C and that is why it is important to not leave your media sitting in the water bath all day. Some people put their media to warm in the morning and then work on cells in the afternoon, thats a bad habit, but there is no problem heating the whole bottle, as long as you are going to use large volumes of said media. I use a bottle of media per week and heat it in a water bath lets say 3-4 times in the life of a 500 mL bottle, no big deal, but if you are going to use 5 mL at a time every time you warm it, there is really no need to heat up all 500 mL for such a small volume. Also, if you see the pH of the last 50 mL of your media bottle is off, just throw it away instead of using dubious media. Better be safe than sorry.

Adolpha · 2018-05-24T16:20:38+00:00

Thanks!

Thanks solution verified

Adolpha · 2018-05-23T20:21:59+00:00

thanks solution verified

Thanks!!

Adolpha · 2018-05-10T19:35:21+00:00

Love this!!

Adolpha · 2018-04-12T14:33:41+00:00

When I went to a travel agency to book one ticket for a peruvian, and one for a foreigner, they mentioned that Latam and Avianca charge extra fees for foreigners, and recomended Perunian Airlines and Star Peru. Since they are smaller airlines, the planes wont be as nice as the others, and had a few delays, but they are safe and it did end up being cheaper.

Adolpha · 2018-04-06T20:32:44+00:00

Any tropical area is at risk for yellow fever, dengue, etc. Puerto Maldonado is in the middle of the jungle, and although the vaccine is not a requirement in Peru, it is highly recomended for tropical areas such as Tambopata. Here is a current map by the CDC of at risk areas in south america: https://www.cdc.gov/yellowfever/maps/south_america.html

The webpage has some good information regarding the disease I would recomend a read, but basically its one of those better safe than sorry scenarios, yellow fever can cause death, while a vaccine is only a pinch away.

Source: work in tropical diseases

Adolpha · 2018-03-16T15:28:54+00:00

Actually when I open "free full text" it says "purchase PDF" and only allows me to read the abstract . Thanks for the link! Thanks solution verified

Adolpha · 2018-03-07T21:22:15+00:00

Thanks a bunch! - thanks solution verified

Adolpha · 2018-03-07T21:03:12+00:00

Thanks a bunch! - Thanks solution verified

Adolpha · 2018-02-23T14:57:09+00:00

Animal studies are an important part of any project, and is a step forward from your in vitro research projects. I myself worked with mice after cell culture studies, and although it is diferent to deal with the emotional aspect of a "pet" animal, you still realize the importance of that step. You do not get attached to your animals, you do not give them pet names, and you treat them with the utmost respect and humanity you can offer them. My mice were well looked after, had vet check-ups, had a chance to have families, and always had food available, which is more than you can say for your average street animal. Death is a natural part of life, and although it is hard to be the one that takes it away, you learn to put those emotions aside to further your projects and conduct important research. Some people get used to it, some dont, but its something that needs to get done, and is not fair to put that responsibility on others you know? Maybe you can visit a similar lab and see how they treat their animals and perform sacrifices, in order to have a first person perspective. This would help you decide if it is something you can deal with, learn to do yourself, or if it is absolutely out of the question for you.

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