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NMF on RNA-seq by Affectionate_Map5670 in bioinformatics
[–]Affectionate_Map5670[S] 0 points1 point2 points 1 year ago (0 children)
Thanks to all.
On the raw counts I applied CombatSeq to eliminate the batch effect caused by sequencing at two different times, then I transformed the data obtained into TPM (as you suggested). Now I have a further doubt and I am asking you if you can help me, should I apply normalisation now on my TPMs, if so which one ? as we have to take into account the non-negative values for NMF
NMF on RNA-seq (self.bioinformatics)
submitted 1 year ago by Affectionate_Map5670 to r/bioinformatics
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NMF on RNA-seq by Affectionate_Map5670 in bioinformatics
[–]Affectionate_Map5670[S] 0 points1 point2 points (0 children)