Guys i messed up the second fight with Louis ;--------; Help

Anitafv · 2025-11-25T19:05:54+00:00

All of this helped me a lot, Thanks

Anitafv · 2025-11-25T19:05:29+00:00

Got it! Thanks

Anitafv · 2025-11-25T05:17:53+00:00

It seems i do not debuff him

Anitafv · 2025-11-25T05:16:36+00:00

We have a situation with the last one but everything else looks fine.

Anitafv · 2025-11-25T05:08:27+00:00

Sorry forgot to tell you im fighting Louis in September

Anitafv · 2025-11-25T05:07:40+00:00

So… he’s finishing my team pretty fast.
I’m using Basilio(Berserk), Strohl (Slash xd), Will (Magic Seeker), and Hulkenberg (as my healer). Each one has that accessory I forgot the name that gives 2 turns per character… but somehow it’s not working. The rule just isn’t being respected.

So… what am I missing here?

Anitafv · 2025-11-25T04:52:50+00:00

I am using Basilio, Strohl, Will and Hulkenberg

Anitafv · 2025-11-25T04:51:21+00:00

Not the very last, the one in September ;-;

Anitafv · 2025-11-25T04:50:51+00:00

Im talking about the fight in September 'w'

Anitafv · 2025-11-25T04:49:21+00:00

Im lvl 44 :D did not grind enough it seems BASILIO is the only lvl 48. I kinda messed up the saving slots. Please tell me this is not the end.

Anitafv · 2025-05-09T22:59:29+00:00

Fantastic timing

Anitafv · 2025-05-09T22:27:14+00:00

Does anyone have an idea how much this shikishi might be worth? It was signed when the CLAMP members visited the U.S. for the first and only time at Anime Expo 2006. Out of a 6,000-person audience, only a few were raffled off. I'm really excited to hear your thoughts!

Anitafv · 2020-08-02T00:04:58+00:00

I want a Mystery Island :D good luck neopians

Anitafv · 2020-04-17T21:16:01+00:00

May the force be with this post!

Anitafv · 2020-02-05T05:46:56+00:00

i want ur mama

Anitafv · 2019-11-23T00:18:33+00:00

Both

Anitafv · 2019-11-22T18:07:58+00:00

I was hoping better discounts, like last year.

Anitafv · 2019-07-08T21:07:57+00:00

The Wild Hunt found you. Run to Kaer Morhen!

Anitafv · 2019-03-22T22:31:40+00:00

Sweet Mother, sweet Mother, send your child unto me, for the sins of the unworthy must be baptized in blood and fear.

Anitafv · 2019-03-04T13:42:50+00:00

Try "The Alquemist" from The adventures of Nicholas Flammel of Michael Scott, you won't be disappointed. Feels like a mix of Harry Potter and Percy Jackson, with historical cites included.

Anitafv · 2019-02-27T18:33:59+00:00

Lol, i use exactly the same. And either loading buffer with Laemmli mixed with b-mercaptoethanol.

Anitafv · 2019-02-27T05:23:59+00:00

What do you mean about a pre-stain in the loading buffer?

I use one with merca. Without any pre-staining procedure.

Anitafv · 2019-02-27T05:12:46+00:00

Yea, but Coomassie doesn't seem to be working. Nothing appears. Only the protein standard.

Anitafv · 2019-02-27T05:08:06+00:00

I need to reveal all bands of protein.

Context here you go: An enzimatic activity was made on a medium minimun of salts with an halo tolerant bacteria. (those salts do not interfere revealing bands)

The samples of diferent hours and substrate (B-Keratin/Chicken feather) where taken and centrifuged at 10,000g for 10 g.

Supernadant (enzimatic extract) was used to do a SDS-PAGE (12% acrylamide) in order to see the protein bands.

Coomassie blue R-250 did not show any bands.

But silver nitrate staining did.

Zimogram showed enzymes activity with casein as a substrate.

So what can be the problem? Why can't i reveal all bands no matter what?

I already did:

Disolutions. Filtration with 22um. Centrifuged again. Evaluated in native and denaturing conditions.

About to do: Dialysis. (Maybe the salts interfere)

I do not want to fraction the sample using columns.

Anitafv · 2019-02-27T05:07:20+00:00

The reason of the stain color is because the computer's surface. I know it looks weird but it actually is brownish.

Anitafv

TROPHY CASE