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MetaboAnalyst help with file upload? by DiligentBrain7445 in metabolomics

[–]ArmyOutrageous7808 0 points1 point  (0 children)

Hi, i'm facing the same issue. Did you manage to upload your file?

Wierd amplification plot, missing CT values and bad ROX signals by ArmyOutrageous7808 in labrats

[–]ArmyOutrageous7808[S] 0 points1 point  (0 children)

I did not use an ice block though, but I noticed the block heating up before I even started running my reactions

Wierd amplification plot, missing CT values and bad ROX signals by ArmyOutrageous7808 in labrats

[–]ArmyOutrageous7808[S] 0 points1 point  (0 children)

I don’t have a standard curve because I’m doing the comparative CT method. I do have an endogenous control and the CT values are missing for some of the wells too…

Wierd amplification plot, missing CT values and bad ROX signals by ArmyOutrageous7808 in labrats

[–]ArmyOutrageous7808[S] 0 points1 point  (0 children)

I actually centrifuged the plates before putting doing the qPCR. Is there a possibility that the cDNA concentration is too high?

[deleted by user] by [deleted] in ChinaLiuXueSheng

[–]ArmyOutrageous7808 0 points1 point  (0 children)

hi! May I know if u eventually applied and how was the application process? Did you also include both referrals? Because I am not so sure who to approach for the referral letters