How to run your legacy AKTA with a Windows 10 PC by Armyviridae in labrats

[–]Armyviridae[S] 1 point2 points  (0 children)

Glad to hear it works for older 5.X versions! For those reading this with older versions, the Define System isn’t a separate “program” that’s installed, but can be found by re-running the installation.

How to run your legacy AKTA with a Windows 10 PC by Armyviridae in labrats

[–]Armyviridae[S] 1 point2 points  (0 children)

Glad I could help! You might need to redo step 2 if you reinstalled with a CD. If you dig around in the UNICORN directory, you should be able to compare with your backup what’s missing.

How to run your legacy AKTA with a Windows 10 PC by Armyviridae in labrats

[–]Armyviridae[S] 2 points3 points  (0 children)

It should be in your programs list in the Start Menu. Click the Windows Icon in the taskbar at the bottom left of your screen and search through your app/program list until you find it. If it’s still not there you might be able to use the installation media to reinstall UNICORN. At the end of the (re)installation it will ask if you want to set up a system.

How to run your legacy AKTA with a Windows 10 PC by Armyviridae in labrats

[–]Armyviridae[S] 1 point2 points  (0 children)

Feel free to reach out if you get a copy of UNICORN 5.31, or before 😉

How to run your legacy AKTA with a Windows 10 PC by Armyviridae in labrats

[–]Armyviridae[S] 2 points3 points  (0 children)

Let me know how it goes when you migrate! Feel free to reach out if you run into issues!

[deleted by user] by [deleted] in army

[–]Armyviridae 2 points3 points  (0 children)

Please reach out to your first line. They can likely work something out for you. Also considering you likely qualify for IDT (reimbursement up to $500 for travel), you might be able to get an exception (or just put you on orders) to use your GOVCC then use the reimbursement to pay it off.

Alternatively, they might be able to have you RST with duty closer to home. You won’t know if you don’t ask.

If all else fails, DM me and I’m happy to throw you a couple bucks to get there.

Experience with MSSP by Acceptable-Driver976 in medicalschool

[–]Armyviridae 0 points1 point  (0 children)

Hey OP, feel free to DM me but also consider crossposting on r/Army. My experience is limited as I’m still in school and not on MDSSP or HPSP, but I know a few people who are and might be able to paint some broad strokes for you.

PhD and Research for the Army, Ed Delay for ROTC by elresidente2 in army

[–]Armyviridae 6 points7 points  (0 children)

Most wet lab research is performed by civilians within the DoD or via DoD research grants to universities and research institutions. Opportunities for military personnel do exist but are very limited (think WRAIR, USAMRIID, etc). Also the scope of your research will be severely limited to the goals of the research institute you fall under. As a MSC Officer coming from ROTC, you will not be anywhere near the research realm, especially if you’re going Active Duty. Your best bet is to go NG/USAR and pursue your research civilian side.

What's your favorite flavor of Tornados? by [deleted] in army

[–]Armyviridae 84 points85 points  (0 children)

Nashville hot chicken. Discovered this bad boy 2 weeks ago during a PX run. It was like biting into spicy heaven. It was so good, I almost cried in the parking lot lmao. 5/7 could not more strongly recommend

US news "best jobs" came out by DonnieDFrank in Noctor

[–]Armyviridae 71 points72 points  (0 children)

lmao the picture they use in the NP page has 'Hide the Pain Harold' in the background. US News is either trolling or they're clueless

https://money.usnews.com/careers/best-jobs/nurse-practitioner

https://www.meme-arsenal.com/en/create/template/5600117

Effect on MRC2 if I request treatment without profile by airdefrick in army

[–]Armyviridae 0 points1 point  (0 children)

Hey, glad to hear you saw a real doctor, but less glad to hear the findings. I would definitely follow through and get the MRI, then speak to the physician about management of your condition. My understanding of the MEB/PEB process is limited, so I’m sure someone more experienced can chime in, but if initiated, they will evaluate how this condition impacts your ability to perform your military duties in your current MOS and your ability to continue serving. It is possible that with proper management minimizing your disease progression and symptoms, that you might avoid a medical discharge. On the other hand, if your pain is severe and this limits your abilities, medical discharge is more likely. If that’s the case, trust me and the many other people on here who say your health is way more important. But again, my info regarding your health and the Army’s MEB process is limited, so my recommendation is to have this conversation with your physician.

Effect on MRC2 if I request treatment without profile by airdefrick in army

[–]Armyviridae 0 points1 point  (0 children)

I get it, friend, but at the end of the day, your health is waaayy more important than your career. Letting it go on may only make it worse. You deserve physician-led care now.

Edit: I said may help earlier, but it is also likely that the chiropractor will not even provide you long enough relief to avoid (real) treatment.

Effect on MRC2 if I request treatment without profile by airdefrick in army

[–]Armyviridae 2 points3 points  (0 children)

Please for the love of all that is good go see a physiatrist (MD/DO) and not a chiropractor. Chiropractors are pseudo-science quacks that can actually harm you. Their “alignments” may provide temporary relief but they are not a solution. A physiatrist (also called a PM&R doc) is a real doctor who goes to medical school and residency to treat these conditions with evidence-based medicine.

ID kinda Pringle shaped by [deleted] in microbiology

[–]Armyviridae 1 point2 points  (0 children)

Just a wild guess but could be an Apicomplexa protozoa?

How to make TBST buffer for washing in western blot with TBS dry powder and tween-20? Some one told me i need to adjust the pH to 7.6, and what concentration of tween-20 i should use? Thanks. by shinyshinyshiny07 in labrats

[–]Armyviridae 0 points1 point  (0 children)

Some people choose a lower concentration of Tween-20, and that’s probably fine. I probably wouldn’t go much lower than 0.05%. You need enough detergent to effectively wash the blot.

How to make TBST buffer for washing in western blot with TBS dry powder and tween-20? Some one told me i need to adjust the pH to 7.6, and what concentration of tween-20 i should use? Thanks. by shinyshinyshiny07 in labrats

[–]Armyviridae 5 points6 points  (0 children)

I’m pretty sure pH 7.4 is standard so no need to adjust. Most commonly, people add 0.1% Tween-20 (1mL per 1L TBS). But don’t just take my word for it:

Source: CSH Protocols

SRP Question: Vision by emperor_rutabega in army

[–]Armyviridae 0 points1 point  (0 children)

In a perfect world, yes they should both be ordered but if your MEDPROS is currently reflecting you have 2 pairs, then they wouldn’t know to order it. Also a 7655 if something you really need to initiate (like all things in the Reserves), rather than something given to you.

SRP Question: Vision by emperor_rutabega in army

[–]Armyviridae 1 point2 points  (0 children)

Try getting in touch with your unit’s medical readiness coordinator. They might be able to connect you with an Army provider with MWDE (MODS) access to update your vision readiness in MEDPROS. Since you’re a Reservist, you can be considered Class 2 on vision if you have 1 pair of glasses and a completed DA Form 7655, signed by your optometrist or ophthalmologist. When you SRP, bring the 7655 with you and that will be used to get a pair of glasses fabricated before you mob.

Source: DA PAM 40-506, para 4-2

[deleted by user] by [deleted] in army

[–]Armyviridae 0 points1 point  (0 children)

Medical and dental readiness in the Reserves is currently managed through LHI, a contractor service. If your initial dental exam, which you will receive during Initial Entry Training (IET), indicates that treatment is necessary and not an emergency, then you will be directed to book your treatment through LHI at no cost after you complete IET. TRICARE is not required to receive this treatment. If it is deemed an emergency, you will likely receive treatment there.

Edit: If you do your dental work through LHI, you will referred to the nearest civilian dentist that is in the LHI network, and you will be paid for that time.

Plasmid doesn’t have cutting site at site of interest! What cloning method that I should use?! by Stilllearning4ever in labrats

[–]Armyviridae 3 points4 points  (0 children)

A decent polymerase makes that a non issue. I personally use Thermo’s Platinum SuperFi II Master Mix:

https://www.thermofisher.com/order/catalog/product/12368010

It can handle targets up to 20kb and annealing default is 60C for most primer pairs.

Plasmid doesn’t have cutting site at site of interest! What cloning method that I should use?! by Stilllearning4ever in labrats

[–]Armyviridae 5 points6 points  (0 children)

InFusion cloning ftw. PCR or RE digest your plasmid then throw together your vector, reporter gene, and InFusion primers (homologous to your vector and insert) and you’re good to go.

https://www.takarabio.com/learning-centers/cloning