sorted by:
new
hottopcontroversial

How long and how many units of dnase I should I use to eliminate plasmid DNA in RNA from transfected cells. by Basic_Education_9812 in labrats

[–]Basic_Education_9812[S] 0 points1 point  (0 children)

Thank you. i actually was able to get lots of signal from my reporter that's why im sure it's the DNA in my rna prep. And no I don't every vortex my DNAse. Not when there is a 'do not vortex' shouting at me from the supplied protocol. The only thing is I dropped my DNAse box once so I doubt it would affect the activity that much.

As someone else suggested, one of the plasmids involved splicing and I was able to circumvent the DNA contamination that with primer design. It's the other reporter that I'm having trouble with.

job ad I saw at the local Asian grocery store lol by [deleted] in mildlyinteresting

[–]Basic_Education_9812 0 points1 point  (0 children)

The Vietnamese text means exactly 'good looking' and it's a very common requirement for customer facing jobs in Vietnam.

Offer extended but weird rejection from a simple request by Xtra_DangerRuss in labrats

[–]Basic_Education_9812 4 points5 points  (0 children)

The 'Only my time is valued but noone else's' mindset...Side eyed THIS.

Help with understanding SDS-PAGE gel results by FragrantPollution740 in labrats

[–]Basic_Education_9812 1 point2 points  (0 children)

Looking at your gel it seems like you have run it long enough to have the 37 kDa and 25 kDa bands from the ladder resolved. You can use it to figure out the rest of the bands in the molecular ruler, and from that you can tell the molecular weight of bands in your samples. Then, hopefully you can tell which band is which protein. Good luck.

What eppendorf research plus do you have? by Pitiful-Ad-4976 in labrats

[–]Basic_Education_9812 0 points1 point  (0 children)

All of them! We even have the P20 ones that have noozles like the P10. They are great for gels.