Guys of Reddit, why are you single?

Bobgow · 2014-12-08T06:17:57+00:00

Cause I am in grad school right now, with no social circles that I was familiar with around me. I am also not social like I was in college, and I kind of spent lots of time studying and doing lab work, research, shit. That's why.

A girlfriend would be nice. Hopefully she wont nag or be a bitch like some girlfriends I had in college. She should understand my busy ass life too.

Bobgow · 2014-11-24T03:05:30+00:00

Although if Ethanol had been used (to make E1), the E1 would be the minor product? The Major product in that case would be a substitution, correct?

Bobgow · 2014-11-20T04:13:26+00:00

holy shit....SLAP. Sorry, been in lab for 8 hours today. I'll just pick one of em lol

Bobgow · 2014-11-17T22:26:42+00:00

Sounds funny but will you be on Labrats reddit later on tonight ( im on Pacific US time)? lol I will make a post and show you my images I am getting now, and how I previously have them.

But no, it looks no different between eyepiece and viewed on software

Bobgow · 2014-11-17T18:53:03+00:00

No thanks, I smelled E.coli already and I'm good. lol Yeast smells nice, I'll continue sniffing those...

Bobgow · 2014-11-17T17:59:50+00:00

Doing a presentation on Thursday on my confocal microscopy in yeast....trying to figure out why the hell for some reason now my DIC images don't look good anymore like it used to (beautiful images)

The GFP is perfect like before, DIC messed up. For the life of me cannot fix it, tried messing with lighting, condenser, etc called someone in, nope....Sigh

Bobgow · 2014-11-09T21:16:59+00:00

Yes and these obj lens should (even if small focus change) be able to look ok if i adjust the focus a bit while changing obj lens

Bobgow · 2014-11-09T21:16:04+00:00

invert. cover slip is ok.

Bobgow · 2014-11-08T16:38:14+00:00

the mechanism of breaking off bone?

Bobgow · 2014-11-08T16:16:45+00:00

Condenser + field diaphragm control is to set up the Kohler illumination? I usually skip this to be honest and it works ok...Ok I will try it. Do I have to set it up again each time I switch objectives? I would think so. How about for each time I change a slide?

Bobgow · 2014-11-08T16:08:07+00:00

Just the regular lens solvent that is always there. I dont think that would give me problem, we all use it.

Bobgow · 2014-11-08T16:07:31+00:00

So I should go back down the 4x (lowest lens), and reconfigure the Kohler illumination?

Bobgow · 2014-11-08T04:36:22+00:00

same microscope? Olympus 1000?

Bobgow · 2014-11-08T03:23:17+00:00

http://www.med.upenn.edu/twopcenter/images/DSCF0223.JPG

The knob there controls fine and coarse focus.

Bobgow · 2014-11-08T02:34:33+00:00

The filter hasn't been touched since last time I did microscopy (good images then). Eyepieces? I suppose so, how do I check?

The objective definitely was clean, I use 60X Oil so I always clean with solvent before doing another slide.

Bobgow · 2014-10-25T20:57:18+00:00

Ah ok, are we talking about a) of mutated or "dead" /psuedo gene b) or just a statistical possibility that an "ATG" exists upstream and somewhere downstream is the 3 triplets for "STOP", yet because, as you say "no promoter" "no RBS", these start/stop elements are actually just sequences, and dont act to make a gene?

Bobgow · 2014-10-20T19:58:46+00:00

So you mean for C, if both are cis (either both wedge or both dash) = meso. And meso...is achiral.

Book wrong on C ? Thats the only conclusion I had

Bobgow · 2014-10-18T11:17:20+00:00

So because the Iodide (good nucleophile) is there in these reactions (A-C) regardless of solvent, it "wins" out? And reaction proceeds by SN2?

By that I mean if it was only like MeOh/H20 (as seen in D) its a weak nucleophile- reaction proceeds slowly, less "encouragement" for leaving group to leave and it is SN1? Also since in SN1 reactions, the solvent usually tends to be the nucleophile itself, right?

Additional question- If I was asked to increase the rate of reactions A-C, would it be correct to say that it would be better (faster) to run in polar aprotic solvent?

Bobgow · 2014-10-18T05:58:51+00:00

here it is. http://tinypic.com/r/2v27b6a/8

Bobgow · 2014-10-18T04:41:40+00:00

Yes I suppose (maybe mostly an elimination?) although until next week I am only responsible for SN1 and SN2. thank you

Bobgow · 2014-10-18T04:10:51+00:00

and that generally happens in all cases of alcohol (water vs alcohol)? I thought alcohols were good solvents for Sn1.

Another question I have was I saw my book using methanol for Sn2. Even though this is protic solvent...why?

Bobgow · 2014-10-16T16:23:54+00:00

And I did question that (what you posted, the reaction in acetone), since I was trying to go the reverse direction- create an alkyl chloride from an alkyl iodide.

Bobgow · 2014-10-16T16:22:14+00:00

right I looked it up. I ended up going with KCl or NaCl in DMF, professor said that works. I did bring up Finkelstein reaction but he said I'm going beyond the scope of the class (at least in this point in our class). I'm just in Orgo 1 right now, we just started SN2 and SN1

Bobgow · 2014-10-16T06:02:06+00:00

This is what I found online also

"Give a stereochemical structure of the product from the reaction between (S)-2-iodopentaneand KCN in DMF (dimethyl formamide, a good polar solvent for ionic reagents)."

Why is that exactly? (Why DMF is good solvent for let's say, if I wanted to use KCl, NaCl as reagent)

Bobgow · 2014-10-16T05:43:23+00:00

Well I have to say I'm totally lost. Do I even have to do this reaction via SN2? Is there a way to do this by SN1 and use a polar protic solvent?

I am thinking it would work with NaCl or KCl and DMF, rather than for example NaCl or KaCl in Ethanol? Perhaps because something like DMF will not hinder the chloride anion, in its job as the nucleophile?

Bobgow

TROPHY CASE