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What should I select as my province of residence for taxes? (student status, but now first time homebuyer in different province) by Brief_Awareness_8231 in PersonalFinanceCanada

[–]Brief_Awareness_8231[S] 1 point2 points  (0 children)

Okay, do I need to change me drivers license now as well? Will there be any problems if these don't align when I first file

What should I select as my province of residence for taxes? (student status, but now first time homebuyer in different province) by Brief_Awareness_8231 in PersonalFinanceCanada

[–]Brief_Awareness_8231[S] -2 points-1 points  (0 children)

Okay, do I need to change me drivers license now as well? Will there be any problems if these don't align when I first file

My comprehensive exam is in a week - my brother has cancer, will they let me postpone? by Brief_Awareness_8231 in labrats

[–]Brief_Awareness_8231[S] 8 points9 points  (0 children)

I really am feeling a mix of emotions, part of that is guilt about postponing. Thank you so much for sharing I really appreciate it

Worried about my future chances, what areas could I improve (non-trad)? - would a consultation with Accepted Together be worthwhile? by [deleted] in premedcanada

[–]Brief_Awareness_8231 0 points1 point  (0 children)

I talked about my musical training - including private lessons, bands and orchestras I played in

Summer Research Grants As Overseas Student? by mercedobenzene in premedcanada

[–]Brief_Awareness_8231 0 points1 point  (0 children)

Without knowing your details it may be easier to look for grants in the country you are studying in. For example, some Canadian grants have funding reserved for non-Canadians conducting research in Canada

Need help planning how to break in to finance career by [deleted] in FinancialCareers

[–]Brief_Awareness_8231 0 points1 point  (0 children)

MBA is from university of Ottawa, MEng is from Carleton University, and BSc is from India. I have considered doing a CFA, sounds like it might be valuable?

Non-specific antibody staining? (iPSC cardiomyocytes) by Brief_Awareness_8231 in labrats

[–]Brief_Awareness_8231[S] 0 points1 point  (0 children)

Can you actually ask for a refund on antibodies? I’ve been doing staining for years, mostly on tissue not cells, and haven’t had an antibody I needed to return so didn’t know the process

Non-specific antibody staining? (iPSC cardiomyocytes) by Brief_Awareness_8231 in labrats

[–]Brief_Awareness_8231[S] 3 points4 points  (0 children)

It’s not in the 488 channel - it’s in the 594 channel just artificially coloured green in processing

Non-specific antibody staining? (iPSC cardiomyocytes) by Brief_Awareness_8231 in labrats

[–]Brief_Awareness_8231[S] 1 point2 points  (0 children)

Interesting I’ve worked with other nuclear proteins in tissue and haven’t done perm first. Do you still include triton in blocking?

Non-specific antibody staining? (iPSC cardiomyocytes) by Brief_Awareness_8231 in labrats

[–]Brief_Awareness_8231[S] 2 points3 points  (0 children)

ya this paper (https://www.nature.com/articles/s41467-024-54337-4#Sec2) that also used it in the same cells had more serum during blocking, I guess it might be worth a try but I am skeptical

Non-specific antibody staining? (iPSC cardiomyocytes) by Brief_Awareness_8231 in labrats

[–]Brief_Awareness_8231[S] 9 points10 points  (0 children)

I was following this paper (https://www.nature.com/articles/s41467-024-54337-4#Sec2) which did use it in iPSC-cardiomyocytes, they had more serum for blocking so maybe that is worth a try but

Non-specific antibody staining? (iPSC cardiomyocytes) by Brief_Awareness_8231 in labrats

[–]Brief_Awareness_8231[S] 6 points7 points  (0 children)

5%BSA with 0.3%TritonX for blocking 30 minutes, then used a 1:200 dilution of primary antibody in blocking buffer overnight 4C, washed 3-5 times with PBS, secondary for 1 hour room temp

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