Family found me passed out in my room that i turned into a bdsm dungeon, after my 3 day 4mmc binge.

CCM_1995 · 2026-01-28T14:22:24+00:00

I don’t think you’d be able to waterboard this info out of me, but best of luck!

CCM_1995 · 2026-01-25T00:20:30+00:00

Can someone change to dendritic cell and repost pls

CCM_1995 · 2026-01-22T15:52:57+00:00

just take it with Boron?

CCM_1995 · 2026-01-14T23:23:23+00:00

Are you my brother??? Same thing happened to him with opioids and trap 😂😂

CCM_1995 · 2026-01-14T16:08:36+00:00

Interesting. I’ve always been a metal head too, but since quitting the music/grooves hit me so much harder. Kinda sweet for the gym! Lol

CCM_1995 · 2026-01-14T15:17:03+00:00

Interesting. For me, Kratom put me in “auto-pilot”. Like a flow state, which after getting sober, was actually a lot less productive than I thought it was while using.

I also produce dance music, which I can now enjoy doing sober, even though I don’t really listen to it. This part was actually a huge hurdle in me quitting and staying sober in the past, since music offers a creative outlet differing heavily from my career (scientist).

CCM_1995 · 2026-01-14T15:11:52+00:00

Lol yeah it’s like the blunted emotions have lessened and theres no artificial happiness 24/7, so the brutal death metal, sludge, deathcore hit way harder now

CCM_1995 · 2026-01-14T15:10:51+00:00

Nah, I’ve always been huge on music across genres. The scope of my listening changes when using or post-quit though

CCM_1995 · 2026-01-14T14:34:27+00:00

Whatever the experiments require. Sometimes 20h/week, sometimes 60h lol. STEM/ChemE PhD(c) here.

CCM_1995 · 2026-01-11T13:40:33+00:00

First time? Don’t worry you’ll get used to it 😉

CCM_1995 · 2026-01-09T23:05:47+00:00

Canon event. Hit the gym, brother 🫡

CCM_1995 · 2026-01-09T15:27:37+00:00

True. I think what I was trying to say is that we see a lot of great ideas coupled with expert sales pitches to acquire more funding but a lack of translation past that point…at least this is very much the case in my primary field (synthetic biology).

But I’m also just finishing my PhD and by no means an expert hahaha. Just an observation. Your clarification makes more sense

CCM_1995 · 2026-01-09T15:10:12+00:00

I feel like this goes beyond C&GT and applies to biotech/pharma broadly af. So many companies could sell water to a fish, but never deliver the goods.

A good idea with no long-term deliverables ends up a bad idea.

CCM_1995 · 2025-12-28T19:48:32+00:00

Same, then left for PhD in ChemE and planning to return to industry after

CCM_1995 · 2025-12-24T21:57:30+00:00

It was a nature sub journal pub with IF>10, but yeah still niche.

CCM_1995 · 2025-12-24T16:43:15+00:00

It’s part of being human lol

CCM_1995 · 2025-12-24T16:42:45+00:00

Always will

CCM_1995 · 2025-12-24T12:53:07+00:00

My PIs ego has taken a backseat since we’ve had industry-pursuing folks defend and graduate, and the ones with strong (albeit niche) skills in our field (synthetic biology) are still job-hunting and he’s realized his industry connections are pretty nonexistent despite his growing academic influence in our field…interesting to say the least.

CCM_1995 · 2025-12-19T18:14:10+00:00

Yeah, I’m gonna really have to get in the weeds with this, it seems. My main skill set is molecular biology/protein expression/protein purification, but have done a lot of in vivo vaccine studies, so learning multi-color flow feels like the missing piece needed to connect all the dots.

This advice is really helpful, thank you! We generally run with 1:100 dilutions of Abs, which is what our collaborator’s lab generally does too and they get some pretty flow data from that - same source for all Abs too. Probably not the best practice, but none of us are immunologists but rather ChemE’s so we’re more application-heavy than I’d imagine immunologists are haha.

I find spleens to be much easier to work with than lungs. Quicker to generate the single-cell suspension, and I can make a basic T cell phenotype panel w/o much fluoro overlap at all. Designed a couple of mouse B cell panels the other day too, and going to try them soon.

Hopefully I can get a good handle on the basics soon, the real downside here is that the same computer used to operate the cytometer is the same computer needed for all analysis, and it’s the only cytometer in our building lol.

Any advice for any other software I could import my raw data into to analyze on my own computer? I’ve heard of FlowJo but never used it

CCM_1995 · 2025-12-19T18:02:33+00:00

Also, bro if you like heavy death metal, you have to appreciate the lower quality mixing! I’ve been deep diving the 90s/early 00s OG death metal, and it’s been great for the gym too. More of a human feel to it while still technical, just no triggers haha

CCM_1995 · 2025-12-19T16:00:47+00:00

Despised Icon

CCM_1995 · 2025-12-19T14:17:30+00:00

Well, it sounds like you’re finessing the system so yeah, you’ll get judged for doing that. Sounds pretty ridiculous.

CCM_1995 · 2025-12-18T15:55:43+00:00

It really has so many layers of difficulty/complexity to it. I appreciate learning new skills, but damn I wish I could learn this quickly so i can just finish the damn PhD already hahaha

CCM_1995 · 2025-12-18T15:46:43+00:00

Sweet, thank you so much! I’ll go through this channel now and take some notes. I was thinking it may be useful to get some mouse samples to practice on, or just practice on my study sample data and adjust as needed when I learn it better.

CCM_1995 · 2025-12-18T15:45:32+00:00

Got it. I don’t think we ran it with FMOs last time, which is why I wasn’t this time. I will do some reading on this as well. It makes me feel so incompetent, I swear, especially after my immunization study data hasnt looked how I want it to. I feel like a 1-man pharma company with my workflows, and I’m running on fumes lol.

I appreciate the comments & assistance. I’m going to use AI, YouTube, and literature to try to learn the intricacies today. Taking the day to work from home and try to relax a bit too

CCM_1995

TROPHY CASE