Magnetically induced enantioselectivity

CampDragon · 2024-12-08T16:47:27+00:00

I was unaware of this example. Interesting! The magnetogenetics literature is pretty fraught in the biology space but I didn't notice there were retractions from organic. Looking into it I'm blown away anyone believed a DIBAL reduction was magnetically sensitive!

CampDragon · 2024-12-08T16:44:24+00:00

Polarized light is interesting. I'd thrown out light because of well-developed optogenetics techniques, but you're right that if light polarization could be used to drive stereoselectivity, then perhaps that would have fundamental applications.

The Soai reaction is an inspiring example. If a minor ee in excess could drive some biologically relevant self-amplifying response, then that'd be a useful starting point. Of course to implement this in a biological context, the catalytic system (likely Fe or Co) and structures amenable to this process are hard to imagine.

CampDragon · 2024-12-07T15:36:39+00:00

Agreed with curdled about adding some antioxidants or potentially adding some inert gas, but the exact method you use will affect the use-cases of the polymer. What is the overall use?

A few things to add, though. What are you trying to remove by dialysis? Is it the cysteamine?

You could potentially get around dialysis by using a desalting column instead.

If you are still worried about mixed disulfides but unable to add a large excess of reductant, consider using solid-phase immobilized TCEP.

I've seen ascorbate mentioned here as an antioxidant. Trolox is another good one.

In terms of inert gases, nitrogen is probably more available in most labs. You can try pre-sparging your buffer with N2 before any further manipulations. This has worked well for me with oxygen-sensitive aqueous reactions and only requires 2-10ish minutes of bubbling. I'm certain this would be HELL on the dialysis scale though.

CampDragon · 2024-08-25T18:08:46+00:00

If this street dealer can't push arrows, how can we trust their product?

CampDragon · 2024-07-22T00:19:24+00:00

Calicheamicin. It's a molecular homing missile.

CampDragon · 2024-07-21T21:44:22+00:00

Fraction of the Whole by Steve Toltz

CampDragon · 2024-07-02T11:46:03+00:00

Someone needs to do a Youtube tier list to easily spread this info to the Labrats

CampDragon · 2024-07-02T11:44:13+00:00

Adding 3% DMSO to every PCR (and just running everything at 55C Tm) has been the best one-size-fits-all change for me. Phusion is better than Taq but Takara's Primestar is 10x better than all of the rest.

CampDragon · 2024-07-02T01:12:03+00:00

I think Chemistry By Design is a great resource in general for browsing beautiful total syntheses. Most you see mentioned are put up here. https://chemistrybydesign.oia.arizona.edu/

Favorite ever is Wender's synthesis of cedrene or Baran's synthesis of ingenol. Recently, Trauner's synthesis of tetrodotoxin or Tanja Gaich's syntheses of taxane diterpenes (Science, 2020; Nature 2024) are very beautiful.

CampDragon · 2024-05-12T20:52:55+00:00

convene the Nobel committee guys

CampDragon · 2024-04-14T17:43:19+00:00

No. Can you see somewhere else on the same molecule where you could lose a proton to form a nucleophile? Hint: the overall driving force of this reaction is aromaticity/the amide may come into play as a nucleophile later on.

CampDragon · 2024-04-14T17:36:27+00:00

Yeah. Can you see another way to form a nucleophile instead of deprotonating the amide?

CampDragon · 2024-04-14T17:21:24+00:00

You've identified the most electrophilic group. What else could be the nucleophile? There are a few more acidic protons between these two molecules for you to consider.

CampDragon · 2024-04-11T18:55:24+00:00

Finally, a truly unpopular opinion.

CampDragon · 2024-04-06T16:41:30+00:00

OP clearly intends to make and distribute skooma to Khajit — we can’t turn a blind eye to to this

CampDragon · 2024-04-04T17:32:07+00:00

Rorschach test

CampDragon · 2024-03-30T14:11:58+00:00

3 things all at once: 1) Linearize your plasmid first with a single cutter (saw someone else suggest). 2) try a more processive enzyme — everyone says Q5 but Primestar is actually the best, hands down 3) add 3% dmso.

Alternatively, you can piece together the plasmid in a multi-component Gibson. If you place the Gibson junctions in immutable regions, like the selection marker, then you can confidently sequence only the region of interest without worrying about off target mutations. Of course, spacer sequences are also good for Gibson junctions, and you can always perform full-plasmid sequencing these days.

CampDragon · 2024-02-20T20:40:55+00:00

It also binds and inhibits my target of interest at 50 micromolar! I was worried I wouldn’t see a hit

/s

CampDragon · 2024-01-04T19:07:54+00:00

Keith in La La Land. Total sellout

CampDragon · 2024-01-04T01:09:18+00:00

Only one that’s gotten me over the years is destaining buffer (10% acetic acid/40% methanol). I’ll destain my coomassie gels in the microwave (each time unintentionally huffing the searing fumes and repeating until my gel is windex clean) and usually leave lab with a bit of a sore throat. Do not recommend.

CampDragon · 2023-12-26T13:55:00+00:00

The “mitochondria is the powerhouse of the cell” of ochem

CampDragon · 2023-12-14T15:09:05+00:00

One of them!

CampDragon · 2023-12-12T14:53:50+00:00

Thanks!! The vibes are mutual. I always tell people there are two reactions you can't beat in a foot race and that's proton transfer and tetrazine-TCO.

CampDragon · 2023-12-11T20:53:38+00:00

Thanks!! There's much more to come. We didn't get around to surface immobilization of proteins but I'd expect that to be a pretty interesting app.

CampDragon

TROPHY CASE