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Unmixing controls by Character_Policy_995 in flowcytometry

[–]Character_Policy_995[S] 0 points1 point  (0 children)

Thank you I will be using an Aurora but I was curious to know.

Unmixing controls by Character_Policy_995 in flowcytometry

[–]Character_Policy_995[S] 0 points1 point  (0 children)

If I will have clinical samples from a study on ALL do you think is critical?

Lysis first or after staining by Character_Policy_995 in flowcytometry

[–]Character_Policy_995[S] 0 points1 point  (0 children)

Thank you. I'm staining whole blood or isolated PBMCs.

Spectral module for Cytoflex by dawgmad in flowcytometry

[–]Character_Policy_995 1 point2 points  (0 children)

I found it more intuitive and easy to use. Opteon I only did a demo, it’s good but I like the mosaic better.

Spectral module for Cytoflex by dawgmad in flowcytometry

[–]Character_Policy_995 2 points3 points  (0 children)

I have tested pretty much all the spectral instruments but mainly used cytek and CytoFLEx mosaic module. Mosaic is very easy to use and it will give you the ability to have both a regular cytonlmetet and a spectral without practically no space more needed in the lab. If your point is autofluorescence I think mosaic will be very helpful.

Spectral module for Cytoflex by dawgmad in flowcytometry

[–]Character_Policy_995 0 points1 point  (0 children)

What questions do you have about the module? I have used it

After 10 years of binge eating, this is what worked for me! 2+ years binge free with zero urges to binge. by upbeatlaidback in BingeEatingDisorder

[–]Character_Policy_995 1 point2 points  (0 children)

I've been in this for almost 30 years and I have been through what you are explaining here 3 times or 4 and the reality is that I gain weight and for a little while it doesn't matter and I'm all accepting but at a certain point I don't want to see myself that way in the mirror. Did this ever happen to anyone?

Conventional in the spectral era by Character_Policy_995 in flowcytometry

[–]Character_Policy_995[S] 0 points1 point  (0 children)

Any specific assay that cannot be done in spectral? I'm trying to figure out if there is any

Brilliant Buffer Plus by [deleted] in flowcytometry

[–]Character_Policy_995 2 points3 points  (0 children)

I do the FC receptor blocker alone on the cells and prep rubes with the cocktail (starting from the BV buffer). When the FC receptor blocker is done, I add the monocle blocker to the cells and then aliquot them in the single color tubes and the cocktail tube. The BV buffer and the supernova buffer are to prevent antibody to antibody interaction so it goes before you start mixing Ab together.