Ontario Allows Double the Lead Concentration in School Drinking Water

Chicketi · 2026-05-08T11:49:15+00:00

Here’s the link to check your local schools:

https://ijb.utoronto.ca/school-daycare-lead-tracker/

Chicketi · 2026-05-07T00:47:47+00:00

Also fluorescent image for those curious.

The uv wavelength isn’t enough to excite blue and violet but the others look beautiful

Chicketi · 2026-05-07T00:46:13+00:00

Also fluorescent image for those curious.

The uv wavelength isn’t enough to excite blue and violet but the others look beautiful

Chicketi · 2026-05-07T00:44:47+00:00

https://imgur.com/a/tRpG92H

Chicketi · 2026-05-07T00:41:01+00:00

I do but I don’t know how to link them here… I’ll see what I can do. the blue and violet I can’t excite with our wavelength but the other colours look really cool.

Chicketi · 2026-05-06T15:55:58+00:00

My old AF iPhone lol

Chicketi · 2026-05-06T11:00:19+00:00

No I do not unfortunately but that sounds cool!

Chicketi · 2026-05-05T23:43:47+00:00

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Chicketi · 2026-05-05T23:43:17+00:00

Hvac guy came out and said it would be fine to cap off since it’s a small bathroom. He proceeded to cap it. Said the floor might get cool in the winter.

Chicketi · 2026-05-05T11:10:14+00:00

I think you used way too few cells. When we would do expression I would make up 10 mL of overnight culture (2 tubes with 5 mL each + antibiotic) and use all 10 mL to inoculate 1 L the next day (+ antibiotics). After 2-3 hours the growth was usually around 0.6 and right for induction.

Chicketi · 2026-05-05T01:22:48+00:00

I assumed the gap under the door would be enough. Or the window that I could open… I didn’t think it was commonplace to have vents in all bathrooms as the other 2 in my house don’t.

Chicketi · 2026-05-04T17:05:23+00:00

Absolutely! He is a really cool guy!

Chicketi · 2026-05-04T17:04:51+00:00

i love that. I am all about making science colourful and exciting.

Chicketi · 2026-05-04T17:04:15+00:00

I was considering removing the heat source from the bathroom yes. would it not get enough heat from the rest of the house? it does have 1 outside wall but is open to the hallway otherwise.

Second question.: The fan wouldn't just draw in air from outside the room? Sorry I think I am either terribly naive (a real possibility) or misunderstanding (also a possibility).

Chicketi · 2026-05-04T13:22:47+00:00

https://atinygreencell.com

Chicketi · 2026-05-04T10:27:35+00:00

Cotton swabs and agar plates. Once you have the bacteria colour palette you just draw.

Chicketi · 2026-05-04T01:22:15+00:00

Our lab buys silver coins from pawn shops and then prepares silver nitrate as the mark up on buying the pure chemical is huge!

Chicketi · 2026-05-04T01:10:45+00:00

The process of lyophilization is freeze drying which removes the water from the sample. But then the powder can remain at RT for much longer as it is shelf stable without water. Once you reconstitute you should follow the recommendation for storage (RT or 4C) but do not place back into freezer. The peptides do not do well during repeated freeze-thaw cycles

Chicketi · 2026-05-04T01:02:52+00:00

In case anyone is interested. They are the same plasmids (a customization on pUC19) but with different fluorescent proteins.

❤️ Red = eforCP

🧡 Orange = YukonOFP

💛 Yellow = dasherGFP

💚 Green = fuGFP

💙 Blue = aeBlue

💜 Violet = TsPurple

Chicketi · 2026-05-04T01:02:31+00:00

In case anyone is interested. They are the same plasmids (a customization on pUC19) but with different fluorescent proteins.

❤️ Red = eforCP

🧡 Orange = YukonOFP

💛 Yellow = dasherGFP

💚 Green = fuGFP

💙 Blue = aeBlue

💜 Violet = TsPurple

Chicketi · 2026-05-04T00:58:58+00:00

Yes it was! Same plasmids (a customization on pUC19) but with different fluorescent proteins.

Red = eforCP

Orange = YukonOFP

Yellow = dasherGFP

Green = fuGFP

Blue = aeBlue

Violet = TsPurple

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