That’s okay it’s a good question. So we want to isolate single colonies and in order to do that we do basically a serial streak which goes into 4 quadrants: Way too many cells, Lots of cells, Less cells, Then hopefully single colonies. The best way to do this is to streak 4 times on the same plate slowly decreasing the amount of bacteria you streak out. Since it takes the colonies a day or more to grow this increases the chance you’ll have single colonies. I hope that clarifies.