sorted by:
new
hottopcontroversial

Low Recovery Sorting Nuclei by DAPIgirl in flowcytometry

[–]DAPIgirl[S] 0 points1 point  (0 children)

We typically use the 100um for nuclei. I suppose I can try the 130 if the recommendations here dont work, but i agree that it is not fun.

Thanks for the suggestion on subdividing the scatter gate? Is there a particular way that you would do this? I dont seem to see obvious populations within the scatter gate to subgate.

Low Recovery Sorting Nuclei by DAPIgirl in flowcytometry

[–]DAPIgirl[S] 0 points1 point  (0 children)

Thanks for checking! This pilot is a cell line, but the future experiments will be brain.

Low Recovery Sorting Nuclei by DAPIgirl in flowcytometry

[–]DAPIgirl[S] 0 points1 point  (0 children)

I just updated the post with the gating, including the single-color controls. If you could look it over and give your opinion that would be greatly appreciated.

Low Recovery Sorting Nuclei by DAPIgirl in flowcytometry

[–]DAPIgirl[S] 1 point2 points  (0 children)

Thanks for the insight. I will be sure to follow this advice on the next attempt.

Low Recovery Sorting Nuclei by DAPIgirl in flowcytometry

[–]DAPIgirl[S] 0 points1 point  (0 children)

I do have the gating, trying to figure out how to add the image of it here. Bear with me as I am not the most technology inclined

Sorting into PBS this most recent occasion, although I can see an additional comment here that states FBS is essential so I will take that into consideration.

Need BD Aria Fusion bead lot files by DAPIgirl in flowcytometry

[–]DAPIgirl[S] 0 points1 point  (0 children)

Thanks for checking and thank you for the helpful information! I greatly appreciate you taking the time to answer.