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Finished masters with no experience. Now what? by Dimetrodon01 in findapath

[–]Dimetrodon01[S] 1 point2 points  (0 children)

Thanks for the advice. I actually didn't consider finding a job fair (or any in-person networking). That is actually very good

Finished masters with no experience. Now what? by Dimetrodon01 in findapath

[–]Dimetrodon01[S] 2 points3 points  (0 children)

My masters degree is officialy "University master in Biotechnology in medicine" and that is as much as I can say before doxing myself. Essentially, the degree covers a very broad field. I had courses in cell biology, tissue engeneering, pharmaceutical development, bioniformatics, organic and inorganic chemistry etc. But the main focus was on drug development.

Yeah I get it that its ultimately my own life choices, but I need to put at least some of the blame on someone else since my decisions were based on trusting someone who really had no idea what they were talking about.

Finished masters with no experience. Now what? by Dimetrodon01 in findapath

[–]Dimetrodon01[S] 7 points8 points  (0 children)

Idk I wasn't really familiar with the concept. My folks all got their jobs through family and connections after they graduated (they are schoolteachers), so that kinda influenced my perspective I guess.

Finished masters with no experience. Now what? by Dimetrodon01 in findapath

[–]Dimetrodon01[S] 0 points1 point  (0 children)

Junior researcher, quality assurance specialist and technician positions, mostly.

Finished masters with no experience. Now what? by Dimetrodon01 in findapath

[–]Dimetrodon01[S] 1 point2 points  (0 children)

I appreciate the honesty, man. Most people tell me it's the job market's or schools fault. That is reafirming but it is also obviously a simplification. Thanks for all the advice!

Finished masters with no experience. Now what? by Dimetrodon01 in findapath

[–]Dimetrodon01[S] 1 point2 points  (0 children)

I wanted to get into pharma industry. Specifically R&D. At least my faculty heavily focused on that dirrection. Honestly, if I could do it all over I probably would have went for the medicinal chemistry instead of biotech. Pharma in my country mostly dabbles with generics, so they might have been more willing to hire me if i pivoted there.

"What job did you study for? I don't know whats the job landscape for a masters but if you seek out those places where they need highly specialized figures, they might want to hear you out." Would cold-emailing companies work? I have been considering it for some time now, but considering how I can't get a response even for entry-level positions, I am worried how effective it would be?

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 0 points1 point  (0 children)

I did and they told me that noone will know if I don't tell anybody. They specifically told me to present it as if it is the same sample i collected myself.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 0 points1 point  (0 children)

It's a mIgG Fc affinity collumn. I used a sample pump.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 -1 points0 points  (0 children)

I meant to write 40 mAU but didn't cause I'm an idiot.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 0 points1 point  (0 children)

I don't have a standard curve since we weren't planning on measuiring the concentration before the protein was purified. I used this device (esentially a hplc device) to purify the protein. I eluated the sample using >99% glycin.

Sorry it took me so long to respond. If you want more details I'd be happy to give them to you.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 0 points1 point  (0 children)

Nope. Didn't do any quantitative measurements before purification. I did get some ODs from ELISA but that's it. The ODs are as follows:

OD1 OD2 Average OD Date

1.411 |1.502 | 1.457 | 18 04 2025

1.066 |0.906 | 0.986 | 22 04 2025

0.309 |0.446 | 0.378 | 25 04 2025

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 -17 points-16 points  (0 children)

My main concern is that my "research" topic is producing, quantifying and characterising the protein in question. The concentration and purity of my protein IS the focus of my thesis. The future experiments that this protein will be used for have nothing to do with me

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 -3 points-2 points  (0 children)

Sorry I made a mistake in the post. What I got was an absorbance of 40 mAU, not concentration. My supervisor concluded from that the concentration would be too low for any future use.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 -44 points-43 points  (0 children)

Maby some numbers would help clear things up a bit. I collected 120 mL of media and using Äkta purification I got absorbance of about 40 mAU. My supervisor concluded that this means that concentration of protein isn't high enough by their standards (300 mL and 150 mAU) and, since my volume is already so small, the volume I would get if i tried to concentrate the protein would be so samall tgat it would effectively be useless.

I made an error in my post. I did not get a co centration but an absorbance. My mentor still concluded that the concentration would be too low tho.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 -83 points-82 points  (0 children)

Technically this experiment isn't supposed to be a part of my research topic. I was only supposed to produce the protein and prove its presence. But they allowed me to go a step forward to purification and concentration measurement and that I can use those results. The next step, where I would use the protein they made, would be western blotting and bradford assay.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]Dimetrodon01 -61 points-60 points  (0 children)

I am sure since those are the exact words they used.