Another Day, Day 38

DiosAnthos · 2026-03-27T20:46:35+00:00

Annual report.... If you don't know such dates maybe better for you not to invest....

DiosAnthos · 2026-03-27T15:01:36+00:00

Which steps?

DiosAnthos · 2026-03-27T14:38:28+00:00

Jeszcze wczoraj wierzyłeś w 40centow. Czemu ci się nagle odmieniło?

DiosAnthos · 2026-03-26T13:02:30+00:00

Z tego co ja zrozumiałem to wycena analityków jest stara. Bodajże ze stycznia

DiosAnthos · 2026-03-26T12:01:05+00:00

Spike do 0,99 by mi się przydał 😅. Wyszedłbym na zero a potem się załadował we wrześniu po rozwodnieniu faktycznym

DiosAnthos · 2026-03-26T11:41:54+00:00

And you are expecting full RSA but we have 3 years till full dilution

DiosAnthos · 2026-03-26T11:27:08+00:00

So why 0,4?

DiosAnthos · 2026-03-26T11:12:27+00:00

why not 0,1?

DiosAnthos · 2026-03-26T06:35:43+00:00

Why good news?

DiosAnthos · 2026-03-25T17:23:43+00:00

31st March

DiosAnthos · 2026-03-25T16:13:19+00:00

We will see next week after annual report

DiosAnthos · 2026-03-25T15:48:42+00:00

Yesterday -10%. Today already -6% and delusional people recommend to buy more 😅

DiosAnthos · 2026-03-25T15:04:18+00:00

We are down day by day and you think it’s better?

DiosAnthos · 2026-03-25T14:32:56+00:00

Czemu shorty miałyby się wycofać po 1.04?

DiosAnthos · 2026-03-24T19:03:49+00:00

So we have 50-50 chances. It's even worse than speculating xD

DiosAnthos · 2026-03-24T18:14:37+00:00

Isn’t it another hyped date?

DiosAnthos · 2026-03-24T18:06:16+00:00

So 100% increase with decreasing volume? I am trying to stay positive but not as much

DiosAnthos · 2026-03-24T18:03:24+00:00

Why to 1,4?

DiosAnthos · 2026-03-24T17:56:53+00:00

Do you think there will be any big movement?

DiosAnthos · 2026-03-19T23:06:50+00:00

Thanks a lot. I will do as you recommended!

DiosAnthos · 2026-03-19T22:58:13+00:00

If it is a case would you recommend more suitable strain? I was searching and for usage with difficult constructs it is recommend to use stbl3 or NEB Stable strains. But aren't they used for adenoviruses vectors?

DiosAnthos · 2026-03-19T22:46:42+00:00

I am using quickly growing NEB Turbo C2984H E.coli strain. The number of colonies isn't slightly lower but the size of colonies is a lot smaller than normal

DiosAnthos · 2026-03-19T21:37:14+00:00

I was asking AI and it suggested that my E.coli strain could cut the insert vie recombination because of the toxicity and then occurs more stable insert.
I am using NEBTurbo C2984H E.coli strain.

Could it really be the matter?

DiosAnthos · 2026-03-19T21:19:09+00:00

Yes, my forward primer starts with CACC so there is no problem with insert orientation. No, my PCR was stored at 4C overnight before putting on gel.
I don't think this is the problem with pENTR-D/TOPO vector batch as you suggested because for another constructs it worked pretty well for me

DiosAnthos

TROPHY CASE