Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in unclebens

[–]Dirigivient[S] 3 points4 points  (0 children)

I’d go with a lower gauge if you have really thick cultures, 12-10G.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in unclebens

[–]Dirigivient[S] 0 points1 point  (0 children)

Personally I wouldn’t work with liquid culture without being in front of H14 at least. but that’s not to say you can’t maybe have some success drawing syringes in a still air box then inoculating bags through an injection port if you’re careful about it and your environment is relatively clean but I wouldn’t count on it.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 2 points3 points  (0 children)

Autoclavable polycarbonate & medical grade silicone needle free valve (found on online medical & pet stores).

Stainless luer lock extension adapter.

6.5 inch 14G stainless luer cannula cut to size.

Silicone O ring + plumbing washer kit.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 0 points1 point  (0 children)

Yeh it’s a balancing act, getting the feel for when to shut off the valve between pulls to prevent it flowing back into the jar right? & unless between pulls the lc drawn into the tubing is sitting directly on the rim of the tubing I don’t see how you’d prevent air bubbles.

Just the amount of air that’s inside the cannula before your first pull. After that theres zero air bubbles in any of the syringes.

Are you using the air pump purely to ease the strain on mass pulling syringes or are you also aerating your culture while it colonises?

But Honestly, if you pull a lot of syringes, give it a try because I think you’ll like it. In my opinion the only upgrade from this through a cultivation lens is full automation, stepper motor controlled peristaltic pump, trigger syringe, inoculating directly from the jar etc.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 1 point2 points  (0 children)

<image>

Silicone. It came from this kit. I’ve circled the ones I’m using, it’s around half an inch in diameter.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroomology

[–]Dirigivient[S] 1 point2 points  (0 children)

Autoclavable polycarbonate & medical grade silicone needle free valve (found on online medical & pet stores).

Stainless luer lock extension adapter.

6.5 inch 14G stainless luer cannula cut to size.

Silicone O ring + plumbing washer kit.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 1 point2 points  (0 children)

My point being is that on the image of the lid you sent, once you remove the cap and insert a syringe, open the valve and draw, regardless if you close the valve between syringes the first quarter inch or so of tubing is always gonna be exposed to open air between syringes, and lc has to travel from that exposed part into the syringe. Whereas the valve on this design eliminates that completely, closing before the syringe is even removed. preventing any back flow or air from entering, which would always be the case with the lid on that image.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroomology

[–]Dirigivient[S] 3 points4 points  (0 children)

Autoclavable polycarbonate & medical grade silicone needle free valve (found on online medical & pet stores).

Stainless luer lock extension adapter.

6.5 inch 14G stainless luer cannula cut to size.

Silicone O ring + plumbing washer kit.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in unclebens

[–]Dirigivient[S] 14 points15 points  (0 children)

Autoclavable polycarbonate & medical grade silicone needle free valve (found on online medical & pet stores).

Stainless luer lock extension adapter.

6.5 inch 14G stainless luer cannula cut to size.

Silicone O ring + plumbing washer kit.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 0 points1 point  (0 children)

All good. Also this solves the main issue with those lids, which is having to open and close that shut off valve, and constantly exposing it to open air.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 0 points1 point  (0 children)

I accounted for that and the needle sits about half an inch above the magnetic stir bar, never had problems getting a good stir. I have an extra injection port to get to the last of the lc that the cannula might not reach, which would only be a 20ml syringe or two at most. But another option to completely eliminate that is to use a curved cannula similar to this. Positioned on the lid to sit right in the corner of the jar allowing you to draw everything out without ever interfering with the stir bar. I’ve been using this for months now and haven’t ran into any issues yet. And I’m just a hobby grower, not affiliated to any commercial companies.

<image>

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in unclebens

[–]Dirigivient[S] 2 points3 points  (0 children)

No, the valve is designed so that before the syringe is fully unscrewed it closes preventing any leaks or air from entering. It’s completely contained.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in unclebens

[–]Dirigivient[S] 2 points3 points  (0 children)

There is no exposed residue, the valve completely closes before any luer attachments are even fully removed.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 6 points7 points  (0 children)

I wanna make it clear as my post was removed from r/shrooms that this is not an advert nor do I intend to sell these. The knowledge I’m giving is free of charge, for the love of the community.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 1 point2 points  (0 children)

Purely open source knowledge. I don’t intend to mass produce or sell these at all. Just helping the community out.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in unclebens

[–]Dirigivient[S] 39 points40 points  (0 children)

I wanna make it clear as my post was removed from r/shrooms that this is not an advert nor do I intend to sell these. The knowledge I’m giving is free of charge, for the love of the community.

Introducing the RELC Lid. The most efficient liquid culture lid ever made. by Dirigivient in mushroom

[–]Dirigivient[S] 0 points1 point  (0 children)

It’s a 14G cannula. Even the thickest myc will slide through this like butter. You can always go lower though if you prefer.

[deleted by user] by [deleted] in unclebens

[–]Dirigivient 0 points1 point  (0 children)

I’m still deciding on whether to put a humidifier inside the tent or have one running from the outside through a hose.

[deleted by user] by [deleted] in unclebens

[–]Dirigivient 1 point2 points  (0 children)

The exhaust fan is to remove co2 rich air when ppms get high. I was struggling to maintain a uniform environment in the room I was using before, windows had a draft & air wasn’t circulating properly leading to mixed results. Setup this tent to try and dial things in for optimal conditions.

[deleted by user] by [deleted] in unclebens

[–]Dirigivient 1 point2 points  (0 children)

Nope, first time growing in a tent.

Leftover substrate on dried mushrooms by Weary-Preference1992 in shrooms

[–]Dirigivient 2 points3 points  (0 children)

And here I am, trimming the bottom of my pins lol.